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Antithrombotic fusion protein and gene for coding same

A fusion protein and antithrombotic technology, applied in the field of fusion protein and gene encoding the fusion protein, can solve the problems of complex drug components, easy to produce antagonistic effect, inconvenience, etc., achieve good antithrombotic, do not produce antagonistic response, and use contraindication less effect

Inactive Publication Date: 2014-07-23
哈尔滨今生康健生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, what follows is that the more and more complex the ingredients of the drug, the easier it is to produce antagonism between them, and the more precautions patients should pay attention to, causing a lot of inconvenience and adverse consequences

Method used

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  • Antithrombotic fusion protein and gene for coding same
  • Antithrombotic fusion protein and gene for coding same
  • Antithrombotic fusion protein and gene for coding same

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0010] Specific embodiment 1: The amino acid sequence of the antithrombotic fusion protein of this embodiment is shown in SEQ ID NO:2.

[0011] The gene sequence of the antithrombotic fusion protein in this embodiment is shown in the nucleotide sequence of SEQ ID NO: 1, which was synthesized by a bioengineering company to make a T vector (pMD18-T or pMD19-T) containing the fusion protein gene. T). Digest the T vector with BamH I and Not I double enzymes (the enzyme digestion reaction system is shown in Table 1, the enzyme digestion reaction was reacted at 37°C for 3h, then 1.5% agarose gel electrophoresis, the target fragment was extracted with DNA GEL KIT purified and recovered), and double-digested pcDNA3.1 / V5-His C with BamHI and Not I (the enzyme digestion reaction system is shown in Table 2, and the enzyme digestion reaction was reacted at 37°C for 3h, and then 1.5% agarose gel Electrophoresis, the target fragment is purified and recovered by DNA GEL EXTRACTION KIT); the...

specific Embodiment approach 2

[0045] Embodiment 2: The gene sequence of the antithrombotic fusion protein in this embodiment is shown in the nucleotide sequence of SEQ ID NO:1.

[0046] The gene sequence of the anti-thrombotic fusion protein in this embodiment is based on the characteristics of the expression system to select CHO-dhft-biased codons, and the base sequence encoding the fusion protein gene is redesigned and synthesized by a bioengineering company. In this embodiment, the gene sequence of the antithrombotic fusion protein has a high CG content.

[0047] This embodiment lays a material foundation for further in-depth research on anti-thrombotic fusion proteins.

[0048]

[0049]

[0050]

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PUM

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Abstract

The invention relates to an antithrombotic fusion protein and a gene for coding the same. The antithrombotic fusion protein can achieve favorable antithrombotic effect, can not easily generate antagonistic actions, has fewer limits for use, and is beneficial to recovery of patients. The amino acid sequence of the antithrombotic fusion protein is disclosed as SEQ ID NO:2. The nucleotide sequence of the gene is disclosed as SEQ ID NO:1. The antithrombotic fusion protein can be used for antithrombotic research and treatment.

Description

technical field [0001] The invention relates to a fusion protein and a gene encoding the fusion protein. Background technique [0002] Cardio-vascular disease (CVD), referred to as cardiovascular disease, is a collective term for cardiovascular disease and cerebrovascular disease. A general term for ischemic or hemorrhagic diseases in the heart, brain and systemic tissues caused by hyperlipidemia, blood viscosity, atherosclerosis, and high blood pressure. Among them, the most serious hazards to human health are hypertension, stroke, and coronary heart disease. According to the World Health Organization report in 2000, one out of every three deaths in the world died of cardiovascular and cerebrovascular diseases. About 3 million people die from cardiovascular disease every year in my country, accounting for 45% of the total death toll. [0003] Cardiovascular and cerebrovascular diseases have the characteristics of "high morbidity, high disability rate, high mortality rate...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61P7/02
Inventor 郭玲
Owner 哈尔滨今生康健生物科技有限责任公司