A method for preparing gelatin from bone meal by enzymatic method

Abstract

The invention belongs to the field of gelatin preparation, in particular to a method for preparing high-grade gelatin by enzymatically using bone meal as a raw material. The method of the present invention comprises: decalcifying the degreased bone powder with a particle size of ≤500 μm with hydrochloric acid, separating it by a solid-liquid separator, adding water to the obtained decalcified bone powder and stirring to obtain a slurry, adding inorganic acid to adjust the slurry After the pH value of the material is between 1.5 and 5, add protease for enzymolysis, add alkaline aqueous solution to the slurry obtained after enzymolysis, adjust the pH of the slurry to inactivate the added protease, and heat for extraction to obtain gelatin-containing gelatin. The mixed slurry is subjected to solid-liquid separation by a solid-liquid separator to obtain a transparent gelatin solution, and then filtered, desalted, ion-exchanged, concentrated and dried to obtain high-quality gelatin products.

Description

technical field [0001] The invention belongs to the field of gelatin preparation, and in particular relates to a method for preparing high-grade gelatin by using bone powder as a raw material through enzymatic hydrolysis. Background technique [0002] Gelatin is a protein obtained through degradation and extraction from connective tissues such as animal bones and skins. It is a product of collagen degradation and is mainly used in capsules, food additives and other fields. Bone gelatin is gelatin extracted from animal bones. Its extraction method has been using the traditional alkaline process. The basic process includes: pickling, washing, liming, washing, neutralization, washing, extraction, filtration, evaporation, drying, etc. process. It takes 60-100 days from pickling, liming to extraction in this process, and the production cycle is very long. In addition, this traditional method also consumes a lot of water, which consumes about 1,000 tons of water per ton of gelati...

AI Technical Summary

Problems solved by technology

From the published academic literature, the gelatin products obtained by the enzymatic hydrolysis process using bone powder as raw material cannot meet the requirements of pharmaceutical capsule gelatin, while the production of gelatin from decalcified osteomin often has the end point of the production process that is not easy to control and there are The problem of not being able to mass-produce

[0004] In Chinese patents 94191461.5, 200710018541.7 and 201010557127.5, the production process of osteoin enzymatic preparation of gelatin has been proposed. The process still exists, so the problems of long pickling cycle and many pickling wastewater still exist, and the viscosity index of the obtained gelatin product is also lower than that of gelatin for pharmaceutical capsules, thus limiting its wide application

[0005] In Chinese patents 02149349.9, 02125447.8 and 201110225294.4, the production process of producing gelatin by enzymatic hydrolysis of bone powder is proposed. The production process is characterized in that the bone particles are ground into powder in the form of wet grinding or dry grinding and then enzymatic hydrolysis. The cycle is relatively short, and the amount of waste water is less than that of the bone enzymatic method, but the viscosity of the gelatin product described in the above patent cannot meet the requirements of the pharmaceutical capsule gelatin product.

Images