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Application of Gastric Cancer Gas Marker in Exhalation in Preparation of Gastric Cancer Diagnostic Reagent

A diagnostic marker, gastric cancer technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of inability to distinguish cancer, etc., achieve the effect of convenient detection method and improved cure rate

Active Publication Date: 2016-06-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the current gastric cancer markers in exhaled breath can distinguish between the two stages of cancer

Method used

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  • Application of Gastric Cancer Gas Marker in Exhalation in Preparation of Gastric Cancer Diagnostic Reagent
  • Application of Gastric Cancer Gas Marker in Exhalation in Preparation of Gastric Cancer Diagnostic Reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, collection of breath sample

[0024] The invention screens out the gastric cancer markers in the exhaled breath by analyzing the exhaled air of early gastric cancer patients, advanced gastric cancer patients and normal people. Breath samples were collected from 50 volunteers, including 12 patients with early gastric cancer, 18 patients with advanced gastric cancer, and 20 normal subjects as the control group. Gastric cancer patients were clinically diagnosed using a variety of diagnostic methods, including biopsies, computed tomography (CT) scans, and punctures, while none of the gastric cancer patients had received chemotherapy or other treatments before breath samples were collected. At least 2 analyzes were performed on each subject's exhaled breath. A total of 100 breath samples were used. Before the sample collection, each volunteer should maintain a natural and peaceful mood within 48 hours, and refrain from smoking and drinking within 24 hours, ...

Embodiment 2

[0025] Embodiment 2, the detection of breath sample

[0026] Breath samples were stored at 4°C in a Tedlar airbag, and the next step was performed within 1 hour. The obtained samples were pre-concentrated for the markers in the samples through a 75-micron CAR / PDMS-coated solid-phase microextraction head. The VOCs in the sample were pre-concentrated, and the concentration time was 30 minutes. GC-MS analysis: Put the extraction head in the GC-MS inlet, heat at 280 degrees, desorb for 1 minute instantly, and inject samples in a splitless manner. After 2 minutes, the diverter valve was opened. CD-1MS chromatographic column for chromatographic separation, 1 micron x 60 m x 0.25 mm. Heating program: the initial temperature is 40 degrees, the retention time is 5 minutes, the temperature is raised to 260 degrees at 10 degrees per minute, and the retention time is 10 minutes. The mass spectrometer scans 42-400amu in the full range, the electron bombardment energy is 70eV, the ion...

Embodiment 3

[0035] In this example, the obtained gastric cancer markers in human breath are used for rapid detection of gastric cancer, and the effect is verified:

[0036] By testing breath samples collected from 6 confirmed early gastric cancer patients, 6 confirmed advanced gastric cancer patients and 6 normal persons. Acetone, 2-methylpentane, 3-methylpentane, n-hexane, 3,3-dimethylpentane, 2-methylhexane, 3-methylhexane, n-hexanol, phenyl acetate , hexadecane, tetradecane, and dodecane exist in exhalation, and when menthol is not detected, it indicates that the subject has gastric cancer. At the same time, the concentration of 2,2-dimethylpropionic acid obviously When the concentration of isoprene decreased significantly, the auxiliary diagnosis was gastric cancer. With 2-methylpentane, 3-methylpentane, n-hexane, 3,3-dimethylpentane, 2-methylhexane, 3-methylhexane, n-hexanol, hexadecane When there are more than 6 kinds in the air, it indicates that the gastric cancer of the subject...

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Abstract

The invention discloses application of stomach cancer gas markers in breath to preparation of a stomach cancer diagnostic reagent. The markers are selected from multiple or all of compositions in the following combinations: isoprene, 2,2-neopentanoic acid, hexyl alcohol, 2-methylpentane, 3-methylpentane, normal hexane, 3, 3-dimethyl pentane, 2-methylhexane, 3-methylhexane, menthol, phenylacetate, cetane, tetradecane, dodecane and acetone. The stomach cancer diagnostic reagent is used for diagnosing stomach cancer by detecting the content of the stomach cancer gas markers in breath of a testee, and also can be used for judging that stomach cancer is in an early stage or a development stage. According to the invention, the multiple stomach markers in breath of people are provided for diagnosing stomach cancer, also can be used for distinguishing stomach cancer in an early stage or a development stage, and are beneficial to guidance on clinical treatment.

Description

technical field [0001] The invention relates to the technical field of detection of gastric cancer; in particular, it relates to the use of gas markers of gastric cancer in exhaled breath in the preparation of diagnostic reagents for gastric cancer. Background technique [0002] Survival of cancer patients is greatly improved when cancer is detected and treated early. For gastric cancer, patients diagnosed with advanced disease have an approximately 10% 5-year survival rate, while patients diagnosed with early-stage disease have a 90% 5-year survival rate. However, with the current state of the art, most gastric cancer patients are usually diagnosed with advanced disease. [0003] The earlier cancer is detected, the better the treatment of cancer, and early diagnosis of cancer by detecting specific cancer-related markers in biological samples is the most valuable method. The biological samples include breath and various body fluids. For many major cancers, markers in body...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 崔大祥陈云生
Owner SHANGHAI JIAO TONG UNIV
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