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Porcine reproductive and respiratory syndrome virus (PRRSV) IgM antibody colloidal gold immunochromatographic assay test paper, and preparation method and application thereof

A technology for immunochromatographic detection and respiratory syndrome, applied in immunoassays, measuring devices, analytical materials, etc., can solve the problems of no specific detection, etc., achieve intuitive and easy to distinguish results, improve specificity, and overcome low sensitivity

Inactive Publication Date: 2014-08-06
WUHAN CHOPPER BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Colloidal gold immunochromatographic test strips for porcine reproductive and respiratory syndrome antigens and antibodies are more common, but there is still no colloidal gold immunochromatographic test strip for specific detection of porcine reproductive and respiratory syndrome IgM antibodies

Method used

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  • Porcine reproductive and respiratory syndrome virus (PRRSV) IgM antibody colloidal gold immunochromatographic assay test paper, and preparation method and application thereof
  • Porcine reproductive and respiratory syndrome virus (PRRSV) IgM antibody colloidal gold immunochromatographic assay test paper, and preparation method and application thereof
  • Porcine reproductive and respiratory syndrome virus (PRRSV) IgM antibody colloidal gold immunochromatographic assay test paper, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Schematic diagram of the structure of the porcine reproductive and respiratory syndrome virus IgM antibody colloidal gold immunochromatography test card figure 1As shown, the horizontal surface of the test strip in the test paper card is sequentially divided into water absorption area 1, solidified antibody area 2, gold standard probe area 3, and sample absorption area 4 from left to right. After the test strip is loaded into the card case 6, it is the porcine reproductive and respiratory syndrome virus IgM antibody colloidal gold immunochromatographic detection test paper card; water absorption area 1, solidified antibody area 2, gold standard probe area 3, sample absorption area 4, The bottom plate 5 is made of water-absorbing filter paper, nitrocellulose membrane, glass fiber membrane, polyester fiber membrane and polyethylene plate, and the cartridge 6 is a plastic cartridge with a viewing window and a sample hole. The detection line T 7 of the solidified antibody a...

Embodiment 2

[0041] Except that the amount of anti-pig IgM monoclonal antibody coated on detection line T 7 is 5 μg / cm; the amount of goat anti-mouse IgG coated on control line C 8 is 6 μg / cm; The labeling amount of the mouse anti-porcine reproductive and respiratory syndrome virus monoclonal antibody is 8 μg / mL, and the coating volume is 20 μL of gold-labeled probe / cm, and the method for colloidal gold-labeled mouse anti-porcine reproductive and respiratory syndrome virus monoclonal antibody : Take 20 mL of colloidal gold with a radius of 40 nm and a pH value of 8.2 and a concentration of 0.01%, add 160 μg of mouse anti-porcine reproductive and respiratory syndrome virus monoclonal antibody, make it combine under stirring conditions, add BSA to a final concentration of 8%, Centrifuge to remove unbound mouse anti-porcine reproductive and respiratory syndrome virus monoclonal antibody, unstabilized colloidal gold particles and aggregates, and dissolve the deep red precipitate at the bottom o...

Embodiment 3

[0043] The method of using the above-mentioned porcine reproductive and respiratory syndrome virus IgM antibody colloidal gold immunochromatography test paper card:

[0044] Dilute 3-4 drops of the serum sample to be tested with 1mL of 0.01M PBS with a pH value of 7.4, then drop 4-5 drops into the sample hole, and judge the result within 10-15 minutes. The red color of the control line C indicates that the test paper card is valid. Both the test line T and the test line T appear red, indicating that the sample IgM antibody is positive, and only the control line C appears red, indicating that the sample IgM antibody is negative.

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Abstract

The invention discloses a porcine reproductive and respiratory syndrome virus (PRRSV) IgM antibody colloidal gold immunochromatographic assay test paper, and a preparation method and application thereof. The test paper comprises a sample absorption region, a colloidal gold labeled probe region, a curing antibody region, a water absorption region, a backboard and a shell, wherein the sample absorption region, colloidal gold labeled probe region, curing antibody region and water absorption region are sequentially attached to the backboard and arranged in the shell in a superposition mode. Purified PRRSV is coated on the sample absorption region; a colloidal gold labeled anti-PRRSV monoclonal antibody is coated on the colloidal gold labeled probe region; and the curing antibody region is sequentially provided with a detection line T coated mouse anti-pig IgM monoclonal antibody and a control line C coated goat anti-mouse IgG. The test paper is simple to operate, has the advantages of favorable repetitiveness, high sensitivity, quick and visual results, simple technique and low cost, can implement mass preparation, is suitable for mass field detection for basic level, and can be used for early diagnosis on swinery PRRSV infections.

Description

[0001] technical field [0002] The invention relates to a detection test paper card and a preparation method thereof, in particular to a porcine reproductive and respiratory syndrome virus IgM antibody colloidal gold immunochromatographic detection test paper card and a preparation method and application thereof. Background technique [0003] Porcine reproductive and respiratory syndrome, also known as blue ear disease, is a severe disease characterized by high fever, adult pig reproductive disorders, and piglet respiratory disorders caused by porcine reproductive and respiratory syndrome virus (PRRSV). Infectious diseases, due to their high pathogenicity and high mortality, the virus has been constantly mutating in recent years, seriously endangering the development of the world's pig industry. [0004] There are many detection methods for this disease, and the serological detection methods mainly include ELISA and serum neutralization test, etc., but these methods have th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531
CPCG01N33/56983G01N33/558G01N2333/135G01N2469/20
Inventor 牟林琳刘洁李建漆世华朱薇温文生谢红玲冯钊
Owner WUHAN CHOPPER BIOLOGY