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Chrysanthemum morifolium tissue culturing and breeding method

A technology of tissue culture and chrysanthemum is applied in the field of plant tissue culture, which can solve the problems of affecting the excellent genetic traits of seedlings, easily mutating in proliferation, and unable to effectively remove viruses, etc., so as to improve tissue culture procedures, improve differentiation and proliferation. rate, the effect of less virus

Inactive Publication Date: 2014-08-13
CHINA JILIANG UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0006] At present, the research reports on the tissue culture of Chrysanthemum chrysanthemum both at home and abroad all use leaves, petioles, hypocotyls, stems and petals as explants for tissue culture of Chrysanthemum chrysanthemum (Luo Zijuan, 1987; Zhang Yuan, 2008; Zhi Hui, 2004 ; Niu Yanbing, 2007; Zhang Na, 2009), but none of these methods can effectively remove the virus in the body of Chrysanthemum chrysanthemum. The tissues differentiate into adventitious buds, and the adventitious buds obtained through this process are prone to variation during the multiplication process, which affects the original excellent genetic traits of the seedlings

Method used

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  • Chrysanthemum morifolium tissue culturing and breeding method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] (1) Field selection: Select the Hangbaiju base in Tongxiang, Zhejiang with good growth and no pests and diseases.

[0037] (2) Obtaining and inducing culture of sterile materials: Cut the young and tender axillary buds of Chrysanthemum chrysanthemum with good growth and no pests and diseases, rinse with tap water for 0.5h, and then shake for 2mins in an aqueous solution with a volume concentration of 1% dishwashing liquid. Rinse with sterile water; soak in 70% ethanol aqueous solution by volume for 30s on the ultra-clean workbench, then soak in mixed disinfectant for 10mins, and then rinse with sterile water for 3 times; absorb the water on the sterilized filter paper, use Peel off the 0.3mm axillary bud tips with a scalpel, then inoculate the axillary bud tips into a culture bottle containing a cluster bud induction medium, cover the bottle cap and seal the bottle mouth with a parafilm, and place it in an incubator at 24°C in the presence of light. Cultivated under 150...

Embodiment 2

[0041] The effect of embodiment 2 disinfectant on the growth of chrysanthemum chrysanthemum

[0042] 10% mass concentration of hydrogen peroxide (H 2 O 2 ) aqueous solution and 0.1% mercuric chloride (HgCl) 2) two kinds of disinfectants in the aqueous solution replace the mixed disinfectant in step (2) of Example 1 to disinfect the explant material (i.e., axillary buds) of Chrysanthemum Chrysanthemum, and other operations are the same as in Example 1. The effects of induction and growth (that is, observing the growth of axillary bud tips on the clump bud induction medium), the results are shown in Table 1. The results showed that mixed disinfectant and mass concentration of 0.1% HgCl 2 The disinfection effect is basically the same, and the disinfection effect of hydrogen peroxide with a mass concentration of 10% is slightly worse, the pollution rate reaches 10%, and the growth is slow. But the mass concentration is 0.1%HgCl 2 Disinfection has an impact on the induction of...

Embodiment 3

[0045] Example 3 Effects of different hormone combinations on the induction of Chrysanthemum buds

[0046] The axillary buds of A. chrysanthemum were respectively inoculated in MS basic medium (ie, cluster bud induction medium) supplemented with different concentrations of 6-BA and NAA. Other operations were the same as those in Example 1. The results showed that the media with different hormone combinations could induce the formation of shoots to different degrees. Among them, the medium MS+6-BA1.5mg / L+NAA0.2mg / L+100mg / L glutamine is the best, which induces the most regenerated buds and grows quickly. The average bud length is 2.05cm, and the bud induction rate reaches 100%, the seedlings were large and robust, and there was no vitrified seedling phenomenon, and the incision parts of the medium without glutamine showed different degrees of browning.

[0047] Table 2 Induction effects of different hormone concentrations on the buds of Chrysanthemum

[0048]

[0049]

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Abstract

The invention discloses a Chrysanthemum morifolium tissue culturing and breeding method. The method enables a large amount of high-quality Chrysanthemum morifolium seedlings to be obtained within a short period to be obtained in order to meet the needs of the market. The method comprises the steps of aseptic material obtaining, inductive culturing bud propagation and rooting, strong seedling culturing, and rooted seedling transplanting. Axillalry buds are used as an explants, and the tissue culturing method is used to rapidly breed, so the disadvantages of long period, low breeding coefficient, easy infection and propagation of viruses, and the like of routine cutting and division breeding of Chrysanthemum morifolium are overcome; and tissue culture seedlings produced by using the method have the advantages of few viruses, stable heredity and the like.

Description

(1) Technical field [0001] The invention relates to a plant tissue culture technology, in particular to a method for tissue culture and rapid seedling propagation of Chrysanthemum Chrysanthemum, through which large-scale production of fine seedlings can be carried out. (2) Background technology [0002] Chrysanthemum morifolium Ramat (Chrysanthemum morifolium Ramat) is a medicinal and edible Compositae herb produced in Zhejiang, my country. It is one of the eight famous medicinal materials in Zhejiang Province. Hangbai chrysanthemum can not only be used as a medicinal material, but also can be made into a drink for drinking, and can also be used for garden viewing. Hangbai chrysanthemum contains volatile oil, flavonoids, polyphenols, chrysanthemum glycosides, purines, choline, stachydine, vitamin A, vitamin B and amino acids. The research of Chinese and Western medicine shows that Hang Baiju has the functions of dispelling wind and clearing heat, calming the liver and impro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 邹克琴李素芳王为民张兴涛
Owner CHINA JILIANG UNIV
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