Preparation method and application of metal ion medium diosgenin imprinted polymer

A technology of imprinting polymer and diosgenin, which is applied in the fields of steroids, chemical instruments and methods, organic chemistry, etc., can solve the problems of insufficient selectivity of adsorbents, high cost of extraction and separation, complicated separation process, etc. The effect of practical value, simple method and simple separation process

Active Publication Date: 2014-08-13
河北汇正生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, diosgenin is mainly separated and purified from turmeric. The adsorbents used in conventional separation and purification technologies often have many problems such as insufficient selectivity, complicated separation process, high extraction and separation costs, low product yield and insufficient purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Add 0.1mmol of diosgenin, 0.45mmol of 4-vinylpyridine, 0.54mmol of cobalt metal ion, 200ul of dimethyl sulfoxide, and 200ul of acetonitrile into the container, and make a pre-polymerization solution by ultrasonication for 18 minutes, then let it stand After 22 minutes, add 1.1mmol EDMA and 13.6mgAIBN, pass argon for 19 minutes, mix well, seal, and react in a water bath at 60°C for 24 hours;

[0019] (2) After the polymerization is completed, grind, sieve, settle with methanol and acetone, and then use a methanol-acetic acid mixed solvent with a volume ratio of 9:1 to elute the template molecule diosgenin until the template molecule cannot be detected in the eluent, Vacuum dried for 28h.

[0020] The adsorption performance analysis of diosgenin molecularly imprinted polymers showed that it had selective adsorption performance for diosgenin, and the elution experiments showed that diosgenin in MIP polymers could be effectively eluted. The experimental results show th...

Embodiment 2

[0023] (1) Add 0.1mmol of diosgenin, 0.73mmol of 4-vinylpyridine, 0.31mmol of cobalt metal ion, 200ul of dimethyl sulfoxide, and 200ul of acetonitrile into the container, and ultrasonically prepare a pre-polymerization solution for 11 minutes, then let it stand After 39 minutes, add 1.88mmol EDMA and 19mgAIBN, pass argon for 11 minutes, mix well, seal, and react in a water bath at 60°C for 24 hours;

[0024] (2) After the polymerization is completed, grind, sieve, settle with methanol and acetone, and then use a methanol-acetic acid mixed solvent with a volume ratio of 9:1 to elute the template molecule diosgenin until the template molecule cannot be detected in the eluent, Vacuum dried for 16h.

[0025] The adsorption performance analysis of diosgenin molecularly imprinted polymers showed that it had selective adsorption performance for diosgenin, and the elution experiments showed that diosgenin in MIP polymers could be effectively eluted. The experimental results show that...

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Abstract

The invention discloses a preparation method of a metal ion medium diosgenin imprinted polymer and a method for purifying diosgenin in a yellow ginger rough extracting liquid. The preparation method of the metal ion medium diosgenin imprinted polymer comprises the following steps: adding diosgenin, 4-vinyl pyridine, metal cobalt (II) ions, dimethyl sulfoxide and acetonitrile to a container, and carrying out ultrasonography to prepare a prepolymerization liquid; then standing; then adding EDMA (Ethylene Glycol Dimethacrylate) and AIBN (Azobisisobutyronitrile), introducing argon gas, uniformly mixing, sealing, and reacting in a 60 DEG C water bath for 24 hours; after the polymerization is completed, grinding, screening, and depositing by using methanol and acetone; and then eluting template molecule diosgenin by using a solvent, and carrying out vacuum drying so as to obtain the metal ion medium diosgenin imprinted polymer. The diosgenin molecular imprinted polymer obtained through the preparation method has higher selective adsorption capacity on diosgenin molecules, can be used for the solid phase extraction of diosgenin and is suitable for the need of certain special occasions.

Description

technical field [0001] The invention relates to a preparation method and application of a metal ion-mediated diosgenin imprinted polymer. Background technique [0002] Molecular imprinting technology is a new technology to artificially prepare molecularly imprinted polymers with highly selective recognition properties for target compounds. First, template molecules and functional monomers form functional monomer-template molecule complexes through covalent or non-covalent interactions, and then add cross-linking agents to initiate polymerization reactions through initiators to generate highly cross-linked polymers. Elution removes the template molecules in the polymer, thereby leaving imprinted holes in the molecularly imprinted polymer matrix that match the size, shape and functional groups of the template molecules, that is, the imprinted sites. Molecules are specific and selective. Molecularly imprinted polymers not only have high selective recognition performance simil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08F222/14C08F226/06B01J20/26C07J71/00
Inventor 李辉许苗苗王素素李亚男李志平逯翠梅
Owner 河北汇正生物科技有限公司
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