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A method for detecting drug residues in meat products and its pretreatment kit

A kit and technology for meat products, applied in the method and its pretreatment kit, in the field of detection of drug residues in meat products, to achieve the effects of reducing consumption, convenient pretreatment, and ensuring adsorption effect

Active Publication Date: 2016-01-20
北京华都肉鸡公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem in the prior art that the pretreatment of amantadine, quinolones and sulfonamide drug residues in meat products can only be used for the pretreatment of a single type of drug residue, and then proposes a method for the detection of these three types of drugs The pretreatment method of the company has been integrated, and a kit for the pretreatment method that can simultaneously detect the residues of amantadine, quinolones and sulfonamides has been designed.

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  • A method for detecting drug residues in meat products and its pretreatment kit
  • A method for detecting drug residues in meat products and its pretreatment kit
  • A method for detecting drug residues in meat products and its pretreatment kit

Examples

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Effect test

Embodiment 1

[0031] Example 1 (multi-residue detection of amantadine, ofloxacin and sulfadiazine in chicken)

[0032] (1) The kit includes:

[0033] 50mL centrifuge tubes are polypropylene centrifuge tubes with 0.5ml graduated scale, 20 pieces;

[0034] 10mL centrifuge tubes are polypropylene centrifuge tubes with 0.1ml graduated scale, 20 pieces;

[0035] Solid phase extraction column: mixed cation exchange solid phase extraction column (filler quality is 60 mg, column volume is 6 ml, no activation is required, the extraction supernatant can be directly passed through the column);

[0036] 0.22 micron filter, using nylon membrane or polytetrafluoroethylene membrane needle filter;

[0037] Extract solution: 5% volume of formic acid, 45% volume of methanol, the rest volume is water, 20mL, 20 tubes;

[0038] The first eluent: 3% hydrochloric acid solution, 60mL, 1 bottle;

[0039] Second eluent: chromatographic grade methanol, 60mL, 1 bottle;

[0040] Eluent: ammonia water methanol solu...

Embodiment 2

[0045] Example 2 (multi-residue detection of amantadine, norfloxacin, and sulfamethoxazole in pork)

[0046] (1) The kit includes:

[0047] 50mL centrifuge tubes are polypropylene centrifuge tubes with 0.5ml graduated scale, 20 pieces;

[0048] 10mL centrifuge tubes are polypropylene centrifuge tubes with 0.1ml graduated scale, 20 pieces;

[0049] Solid phase extraction column: mixed cation exchange solid phase extraction column (filler quality is 60 mg, column volume is 6 ml, no activation is required, the extraction supernatant can be directly passed through the column);

[0050] 0.22 micron filter, using nylon membrane or polytetrafluoroethylene membrane needle filter;

[0051] Extract solution: 2% volume of trichloroacetic acid, 48% volume of methanol, the remaining volume is water, 20mL, 20 tubes;

[0052] The first eluent: 5% formic acid solution, 60mL, 1 bottle;

[0053] Second eluent: chromatographic grade methanol, 60mL, 1 bottle;

[0054] Eluent: ammonia water m...

Embodiment 3

[0059] Example 3 (multi-residue detection of ciprofloxacin, enrofloxacin and sulfamethazine in duck meat)

[0060] (1) The kit includes:

[0061] 50mL centrifuge tubes are polypropylene centrifuge tubes with 0.5ml graduated scale, 20 pieces;

[0062] 10mL centrifuge tubes are polypropylene centrifuge tubes with 0.1ml graduated scale, 20 pieces;

[0063] Solid phase extraction column: mixed cation exchange solid phase extraction column (filler mass is 60 mg, column volume is 6 ml, no activation is required, and the extracted supernatant can be directly passed through the column);

[0064]0.22 micron filter, using nylon membrane or polytetrafluoroethylene membrane needle filter;

[0065] Extract solution: 2% volume of trichloroacetic acid, 48% volume of methanol, the remaining volume is water, 20mL, 20 tubes;

[0066] The first eluent: 5% formic acid solution, 60mL, 1 bottle;

[0067] Second eluent: chromatographic grade methanol, 60mL, 1 bottle;

[0068] Eluent: ammonia wa...

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Abstract

The application provides a kit for detecting drug residues in meat. The kit contains a hybrid cation exchange solid-phase extraction column, an extract of adamantanamine, quinolones and sulfanilamide medicines, a first leacheate, a second leacheate, an eluate and a reconstitution fluid. The invention also provides a drug residue detection method by the use of the kit. Pretreatment methods of the above three medicines are integrated to design a kit for simultaneous pretreatment of drug residues of adamantanamine, quinolones and sulfanilamide medicines. The kit can be used for pretreating the above three medicines simultaneously. After pretreatment, chromatography or mass spectrometry of a sample can be carried out by a conventional technology.

Description

technical field [0001] The invention relates to a drug residue detection method, in particular to a method for detecting drug residues in meat products and a pretreatment kit thereof. Background technique [0002] At present, many meat companies have to carry out the residue detection of amantadine, quinolones and sulfonamides. The pretreatment of samples is an important step in residue detection, and it is also the most time-consuming and reagent-consuming step. However, the existing pretreatment for the detection of residues of amantadine, quinolones and sulfonamides in meat products can only be used for the pretreatment of a single drug residue, which is inefficient, takes a long time to process, and consumes a lot of organic reagents. , resulting in high cost for meat enterprises to apply. Contents of the invention [0003] The present invention aims to solve the problem in the prior art that the pretreatment of amantadine, quinolones and sulfonamide drug residues in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
Inventor 王宏卫郭黎明饶钦雄张宝李宗进
Owner 北京华都肉鸡公司