A method of increasing the content of esters in apple brandy
A technology of apple brandy and content, which is applied in the field of wine making, can solve the problems of slow fermentation and insufficient ester substances, and achieve the effects of strong aroma, quality improvement, growth and fermentation rate
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Embodiment 1
[0031] Using Saccharomyces cerevisiae 1023 and 1620 strains (volume ratio 1:1) to ferment and produce apple brandy, the steps are as follows:
[0032] 1. Activation culture of strains: Saccharomyces cerevisiae strains preserved on a slant were streaked on PDA medium for culture at 27°C for 72 hours.
[0033] 2. Liquid culture: the yeast activated for 72 hours was inoculated into PDA liquid medium, and the culture condition was 27° C. for 72 hours.
[0034] 3. Seed solution culture: absorb the seed solution cultivated in the PDA liquid medium and add it to a 1000ml glass bottle containing 800ml apple juice for expansion and cultivation to obtain a fermented seed solution. The culture condition: 27°C, 72h. The soluble solids content of the apple juice used in the present embodiment is 12-14%.
[0035] 4. Fermentation of apple brandy: Add inorganic nitrogen source ammonium chloride 960mg / L and sulfur dioxide content 75mg / L to apple juice, add S. Concentration is 10 6 cfu / mL, ...
Embodiment 2
[0039] Using Saccharomyces cerevisiae 1023 and 1620 strains (volume ratio 1:1) to ferment and produce apple brandy, the steps are as follows:
[0040] 1. Activation culture of strains: take the strains preserved on the slant and carry out streak culture on PDA medium, the culture temperature is 27°C, and the culture time is 72h.
[0041] 2. Liquid culture: the yeast activated for 72 hours was inoculated into PDA liquid medium, and the culture condition was 27° C. for 72 hours.
[0042]3. Seed solution culture: absorb the seed solution cultivated in the PDA liquid medium and add it to a 1000ml glass bottle containing 800ml apple juice for expansion and cultivation to obtain a fermented seed solution. The culture condition: 27°C, 72h. The soluble solids content of the apple juice used in the present embodiment is 13-15%.
[0043] 4. Fermentation of apple brandy: add inorganic nitrogen source ammonium chloride 960mg / L, biotin 1μg / l, sulfur dioxide content 75mg / L to apple juice,...
Embodiment 3
[0047] Using Saccharomyces cerevisiae 1023 and 1620 strains (volume ratio 1:1) to ferment and produce apple brandy, the steps are as follows:
[0048] 1. Activation culture of strains: take the strains preserved on the slant and carry out streak culture on PDA medium, the culture temperature is 27°C, and the culture time is 72h.
[0049] 2. Liquid culture: the yeast activated for 72 hours was inoculated into PDA liquid medium, and the culture condition was 27° C. for 72 hours.
[0050] 3. Seed solution culture: absorb the seed solution cultivated in the PDA liquid medium and add it to a 1000ml glass bottle containing 800ml apple juice for expansion and cultivation to obtain a fermented seed solution. The culture condition: 27°C, 72h. The soluble solids content of the apple juice used in the present embodiment is 13-15%.
[0051] 4. Fermentation of apple brandy: Add 960 mg / L of inorganic nitrogen source ammonium chloride, 10 μg / l of biotin, and 75 mg / L of sulfur dioxide to th...
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