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Method for coupling extraction and fermentation to separate 2,3-butanediol in fermentation broth

A technology of butanediol and fermented liquid, which is applied in the field of bioengineering, can solve the problems of difficult recycling and excessive waste water discharge, and achieve the effects of avoiding high salt concentration, reducing treatment and discharge, and low energy consumption for separation

Active Publication Date: 2015-12-30
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The purpose of the present invention is to propose an extraction and fermentation coupling separation fermentation method for the above-mentioned problems such as difficulty in recovering high-concentration salt in the lower phase and excessive waste water discharge during the separation of 2,3-butanediol in the fermentation broth by the current salting-out extraction method. A new method for 2,3-butanediol in the liquid, the method adds sugar or sugar and a small amount of inorganic salt to the fermentation broth to extract 2,3-butanediol, and the lower phase after extracting 2,3-butanediol is diluted It is directly used in the fermentation of 2,3-butanediol to realize the reuse of the lower phase, which avoids the problem of salt recovery in the lower phase of salting-out extraction, and is a promising method for separating 2, 3-butanediol method

Method used

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  • Method for coupling extraction and fermentation to separate 2,3-butanediol in fermentation broth
  • Method for coupling extraction and fermentation to separate 2,3-butanediol in fermentation broth
  • Method for coupling extraction and fermentation to separate 2,3-butanediol in fermentation broth

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preparation example Construction

[0035] 2. Preparation of 2,3-butanediol fermentation broth:

[0036] (1) Inoculate Enterobacter cloacae into the seed culture medium, and culture it on a shaker at 35-38° C. and 200-300 rpm for 12-18 hours;

[0037] (2) Insert the bacterium culture liquid of step (1) into the fermentation medium according to the inoculum amount of 10%, and culture it on a shaker at 37°C and 200rpm for 48-90h to obtain 2,3-butanediol fermentation liquid, and the fermentation ends When the residual sugar concentration is 10~15g / L.

[0038] In a specific embodiment, the bacterium culture solution in step (1) can also be fermented in a fermenter, specifically: with Biotech5L full-automatic fermenter, the bacterium culture solution in step (1) is inoculated with 10% inoculum Put it into 2L fermentation medium, control the pH to 5.8 with 5mol / L NaOH, and ferment at 37°C and 250r / min. Keep 200ml / L in the fermentation process. min compressed air, the initial concentration of glucose is 135g / L, soli...

Embodiment 1

[0053] The influence of different sugars of embodiment 1 on the separation effect of 2,3-butanediol

[0054] According to the method described in step 1)-3) in the above-mentioned 3 (from 2,3-butanediol fermentation broth or 2,3-butanediol fermentation liquid, the method for isolating 2,3-butanediol) from 2 , 2,3-butanediol was separated from 3-butanediol fermentation supernatant. Wherein, the concentration of 2,3-butanediol in the 2,3-butanediol fermentation clear liquid is 85.54g / L, and in the 2,3-butanediol fermentation clear liquid, add different kinds of sugar (the kind of sugar, such as Table 1) After dissolving, add different organic solvents (organic solvent types, such as Table 1), mix, stand at room temperature, separate phases, take samples to measure the concentration of 2,3-butanediol in the upper phase and the lower phase, and calculate 2 , the partition coefficient of 3-butanediol (K BD ) and recovery (Y); and measure compared to (R), the results are shown in ...

Embodiment 2

[0058] The influence of embodiment 2 different organic solvents on 2,3-butanediol separation effect

[0059] According to the method described in step 1)-3) in the above-mentioned 3 (from 2,3-butanediol fermentation broth or 2,3-butanediol fermentation liquid, the method for isolating 2,3-butanediol) from 2 , 2,3-butanediol was separated from 3-butanediol fermentation supernatant. Among them, the concentration of 2,3-butanediol in the 2,3-butanediol fermentation liquid is 69.70g / L, and the 2,3-butanediol fermentation liquid is added to the 2,3-butanediol fermentation liquid 15% and 30% of the mass of glucose are dissolved to obtain a sugary mixture. Add different organic solvents (types of organic solvents, as shown in Table 2) to the two kinds of sugar-containing mixed liquids respectively, and mix them. The volume ratio of the organic solvents to the 2,3-butanediol fermentation clear liquid is 1:1. Separate the phases, take samples to measure the concentration of 2,3-butan...

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Abstract

The invention discloses a method for separating 2,3-butanediol from fermentation broth by coupling of extraction and fermentation. The method comprises the steps of adding a certain amount of sugar and / or inorganic salts into 2,3-butanediol fermentation broth and dissolving to obtain a mixed solution containing sugars; adding an organic solvent to the mixed solution containing sugars, mixing by shaking, standing the mixed solution at 15-40 DEG C or centrifuging until two phases are separated out, namely, the top phase is a 2,3-butanediol-enriched organic phase and the bottom phase is a sugar-enriched aqueous phase; distilling the top phase under a reduced pressure at a temperature below 105 DEG C to remove the organic solvent and water to obtain the crude 2,3-butanediol; removing the organic solvent from the bottom phase, adding water and diluting until the sugar concentration of the solution is 80-200g / L, inoculating a thallus and carrying out 2,3-butanediol fermentation. The method disclosed by the invention has the advantages of simple operation and low separation energy consumption, the coupling of fermentation and separation is achieved and the method is a method for separating 2,3-butanediol from fermentation broth with an industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to the separation technology of microbial fermentation liquid, in particular to a method for separating 2,3-butanediol in fermentation liquid by coupling fermentation with extraction technology. Background technique [0002] 2,3-Butanediol is an important chemical raw material and liquid fuel, which is widely used in many fields such as chemical industry, medicine, food, fuel and aerospace. 2,3-butanediol is dehydrogenated to diacetyl, which is a food flavor additive and antibacterial additive. 2,3-butanediol is catalyzed by dehydration to form methyl ethyl ketone, which is a high-efficiency liquid fuel additive and a good low-boiling point solvent. To synthesize rubber, polyurethane and other materials; the esterified form of 2,3-butanediol can be used in drugs, cosmetics, detergents, etc. 2,3-Butanediol has a high combustion value and a very high octane number. It is a high-qua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C31/20C07C29/86C12P7/18
CPCC07C29/86C12P7/18C07C31/207
Inventor 戴建英刘春娇修志龙
Owner DALIAN UNIV OF TECH
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