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Method for detecting novel testosterone stimulant

A technology for stimulants and testosterone, applied in the field of medical testing, can solve problems such as the inability to detect new testosterone stimulants

Inactive Publication Date: 2014-08-27
王静竹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, previous detection methods have been unable to detect this new testosterone stimulant, and no method is currently available for the detection of this stimulant

Method used

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  • Method for detecting novel testosterone stimulant
  • Method for detecting novel testosterone stimulant
  • Method for detecting novel testosterone stimulant

Examples

Experimental program
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Effect test

Embodiment 1

[0024] 1. Instruments and equipment

[0025] 1 / 100,000 electronic balance: Satorious company, Switzerland;

[0026] Centrifuge: LD5-2A, Beijing Medical Centrifuge Factory;

[0027] Nitrogen blowing head: Wali Medical Instrument Factory, Chaoyang District, Beijing;

[0028] Rapid vortex mixer: Shenzhen Guohua Company;

[0029] Ultrapure water preparation instrument: Milli-Q, MILLIPORE Company;

[0030] Gas chromatography / mass spectrometry (GC / MS): HP6890 / 5973, Agilent Technologies;

[0031] Gas chromatography / combustion furnace / isotope ratio mass spectrometer (GC / C / IRMS): DELTA-V, ThermoFisher Scientific;

[0032] High-performance liquid chromatography (HPLC): Waters2796; automatic collector: Waters Fraction Collector III, Waters Corporation.

[0033]2. Reagents

[0034] Phosphate buffer: gradually add 0.2M dipotassium hydrogen phosphate aqueous solution to 0.2M potassium dihydrogen phosphate aqueous solution, and check the pH value with a pH meter until pH = 7.0.

[003...

experiment example 1

[0047] mixed with 13 Preparation of testosterone materials for C-labeled testosterone reference substance: testosterone for medicinal use was purchased from Shanghai Pharmaceutical Factory, and the raw materials used for the preparation of new testosterone A were testosterone for medicinal use and reference substance T-3,4- 13 C 2 . Gradually add reference substance T-3,4- 13 C 2 , measure the δ value after mixing until its δ value is -18.9‰; the raw materials used to prepare the new testosterone B are medicinal testosterone and the reference substance T-3- 13 c. Gradually add the reference substance T-3- 13 C, measure the δ value after mixing until the δ value is -20.6‰. The new testosterone material is filled into capsules with 80mg in each capsule.

[0048] The 14 subjects in this experiment are male healthy volunteers. It was determined that all subjects had normal hormone metabolism and steroid hormone levels. All subjects were tested and collected urine samples ...

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Abstract

The invention relates to a method for detecting a novel testosterone stimulant. The method comprises the following steps: getting a urine sample, carrying out enzymolysis in constant-temperature water bath, extracting androsterone, removing 13C which is labeled on C3 in an androsterone molecule to obtain ANAD; and dissolving ANAD by tert-Butyl methyl ethe, and analyzing in a isotope ratio mass spectrometer, thereby obtaining the delta value of ANAD, wherein if the absolute value of the difference between the delta value of androsterone and the delta value of ANAD is greater than 1.2 times delta unit value, the sample is positive. The method can be used for detecting a stimulant in competitive sports, is simple and feasible, can be used for rapidly detecting a great quantity of samples, and is flexible in detection and high in accuracy.

Description

technical field [0001] The invention relates to a method for detecting a novel testosterone stimulant, which belongs to the field of medical detection. Background technique [0002] Testosterone (Testosterone), alias: testosterone; testosterone; testosterone; 17β-Hydroxy-3-oxo-4-androstene-17β-ol-3-one, molecular formula: C 19 h 28 o 2 , the average value of the testosterone reference substance δ value is -28‰, and the structure is as follows: [0003] [0004] In competitive sports, the use of doping has become a cancer that betrays the Olympic spirit and seriously affects the healthy development of sports. Among the prohibited substances announced by the World Anti-Doping Agency (WADA), testosterone is an endogenous steroid stimulant with high frequency of use. Since the active ingredients contained in endogenous steroid preparations have the same chemical structure as the corresponding hormones secreted by the human body, the difference between the two lies in thei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 王静竹
Owner 王静竹
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