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Specificity yelk immune globulin composition for preventing pathogenic bacteria and preparation thereof

A technology of egg yolk immunoglobulin and pathogenic bacteria, which is applied in the direction of antifungal agents, antibacterial drugs, antibodies, etc., can solve the problems of large application limitations and small sterilization range, and achieve high safety, good bactericidal effect, The effect of specific yolk immunoglobulin content stabilization

Active Publication Date: 2014-09-03
GUANGZHOU PHGO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of small sterilization range and large application limitations in the prior art, the present invention provides a specific egg yolk immunoglobulin composition and its preparation that are safe and widely used against six kinds of pathogenic bacteria

Method used

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  • Specificity yelk immune globulin composition for preventing pathogenic bacteria and preparation thereof
  • Specificity yelk immune globulin composition for preventing pathogenic bacteria and preparation thereof
  • Specificity yelk immune globulin composition for preventing pathogenic bacteria and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A specific yolk immunoglobulin composition against six kinds of pathogenic bacteria, made by the following method:

[0047] Composite antigen preparation: select group A group B hemolytic streptococci, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Streptococcus mutans and Candida albicans, use Luria-Bertani medium (including 1% Tryptone, 0.5% yeast extract, 1% sodium chloride and 1.5% agar) were cultivated in a three-gas incubator for 18 hours; Beef extract, 1% tryptone, 0.5% sodium chloride, 1.5% agar), cultivated in a constant temperature double-layer shaking incubator for 24 hours at 37°C, and the rotating speed of the constant temperature shaking incubator was 200rpm , and then inactivated for later use; the bacteria that have been cultivated and inactivated are divided into A group B-type hemolytic streptococcus 25%, Staphylococcus aureus 15%, Pseudomonas aeruginosa 15%, Escherichia coli 15%, Streptococcus mutans 15% %, the ratio of Candida albi...

Embodiment 2

[0052] A specific yolk immunoglobulin composition against six kinds of pathogenic bacteria, made by the following method:

[0053] Compound antigen preparation: select cluster A group B hemolytic streptococci, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Streptococcus mutans and Candida albicans, and use Luria-Bertani medium in a trigas incubator at 37°C Then, the above strains were separated and inoculated into the broth medium, and cultured in a constant temperature double-layer shaking incubator at 37°C for 24 hours. The speed of the constant temperature shaking incubator was 200rpm, and then inactivated Standby; the bacteria that have been cultured and inactivated are divided into cluster A, type B hemolytic streptococcus 40%, Staphylococcus aureus 15%, Pseudomonas aeruginosa 10%, Escherichia coli 10%, Streptococcus mutans 15%, Candida albicans The ratio of 10% of the bacteria was mixed evenly to obtain a strain mixture; then the mixed strain mixture wa...

Embodiment 3

[0058] A specific yolk immunoglobulin composition against six kinds of pathogenic bacteria, made by the following method:

[0059]The chickens for enhanced immunization are selected by the following method: use cluster A hemolytic streptobacter to make antigens, immunize hens respectively, inject 1ml antigen each time after the first injection, and then boost injection once every two weeks, a total of three times; On the 7th month after the first injection, mark the eggs produced by these chickens and extract the IgY respectively, and detect the potency of the prepared IgY by ELISA method; Chickens with IgY titer ≥ 1:128 are hatched from eggs produced by these chickens, and they grow up to 2-3 months as chickens for enhanced immunization. All the other step parameters are the same as in Example 1.

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Abstract

The invention relates to a specificity yelk immune globulin composition for preventing pathogenic bacteria. The composition is prepared by preparing compound antigen, performing supplementary immunization on chicken, preparing crude extract and purifying. The invention further relates to a preparation prepared from the composition. The preparation comprises the following components in percentage by mass: 0.15-0.3% of specificity yelk immune globulin composition for preventing pathogenic bacteria or crude extract of the composition, 0.3-0.6% of povidone K30, 1.0-2.0% of glycerol, 0.3-0.5% of polysorbate, 0.10-0.30% of fresher essence, 0.05-0.1% of edible essence and the balance of distilled water of 100%. The specificity yelk immune globulin composition for preventing pathogenic bacteria and the preparation of the composition are high in antibody activity, high in specificity, good in specificity sterilization effect, free of microecological balance interference, free of adverse irritation on human bodies and high in safety performance.

Description

technical field [0001] The invention relates to a specific yolk immunoglobulin and its preparation, in particular to a specific yolk immunoglobulin composition against six pathogenic bacteria and its preparation. Background technique [0002] The application of antibiotics has brought good news to people's health to a certain extent, but due to the large-scale application of antibiotics, it has also brought a series of problems: such as the generation of drug-resistant bacteria, the generation of side effects, and the impact of broad-spectrum antibiotics on beneficial bacteria. killing effect, etc. In order to solve this problem and seek a targeted and safe bactericidal drug, people discovered Immunoglobulin of yolk (IgY for short), which can specifically bind to the corresponding antigen, thereby inhibiting or Change the state and activity of the antigen, and promote the phagocytosis of bacteria by segmented nuclear cells or macrophages through opsonization. [0003] The ...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61P31/04A61P31/10
Inventor 段朝晖蒙晓赵擎宇陈伟良谢民强魏理蒙永祥
Owner GUANGZHOU PHGO BIOTECH
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