Preparation method of artificial esophagus with histological structure

A technology of artificial esophagus and histology, applied in medical science, prosthesis, etc., can solve problems such as unsatisfactory treatment effect, and achieve the effect of prolonging life and reducing pain.

Inactive Publication Date: 2015-06-24
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two types of commonly used substitutes: one is autologous other tissues or organs, such as stomach, jejunum, colon, etc. Among them, the stomach is the most commonly used substitute in domestic esophagus replacement surgery, but these substitutes repair the damage with damage, and at the same time The treatment effect is also not satisfactory, for example, the mortality rate of patients who replace the diseased esophagus with the stomach is 7-20%, and the postoperative syndrome is as high as more than 50%; another type of substitute is plastic, rubber, glass, polyethylene, Teflon, Travelers, etc., the disadvantages of these non-degradable, non-biocompatible alternatives are also obvious

Method used

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  • Preparation method of artificial esophagus with histological structure
  • Preparation method of artificial esophagus with histological structure
  • Preparation method of artificial esophagus with histological structure

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment one: a kind of preparation method of artificial esophagus with histological structure, specifically comprises the following steps:

[0021](1) Take the mucosa and submucosa in the esophagus of animals (such as pigs, dogs, sheep, etc.), wash, cut into pieces, and immerse the mucosa and submucosa in a mixture of NaCl, Tris-HCl, phenylmethylsulfonyl In a solution mixed with fluorine and N-methylmaleimide, the concentration of NaCl in the solution is 3.4mol / L, the concentration of Tris-HCl in the solution is 0.05mol / L, and phenylmethylsulfonate The concentration of acid fluoride in the solution is 2mmol / L, and the concentration of N-methylmaleimide in the solution is 1mmol / L. After homogenization, the homogenate is centrifuged at a low temperature of 4°C at a speed of 8000r / min. 20min, take the supernatant after centrifugation, and immerse the remaining precipitate after centrifugation in a solution mixed with NaCl, Tris-HCl, phenylmethylsulfonyl fluoride and N-m...

Embodiment 2

[0028] Embodiment two: a kind of preparation method of artificial esophagus with histological structure, specifically comprises the following steps:

[0029] (1) Take the mucosa and submucosa in the esophagus of animals (such as pigs, dogs, sheep, etc.), wash, cut into pieces, and immerse the mucosa and submucosa in a mixture of NaCl, Tris-HCl (tris-hydroxymethylamino methane), phenylmethylsulfonyl fluoride and N-methylmaleimide, wherein: the concentration of NaCl in the solution is 3.4mol / L, the concentration of Tris-HCl in the solution is 0.05 mol / L, the concentration of phenylmethylsulfonyl fluoride in the solution is 2mmol / L, and the concentration of N-methylmaleimide in the solution is 1mmol / L. Centrifuge at a speed of 12000r / min for 20min, take the supernatant after centrifugation, and then immerse the remaining sediment after centrifugation into a mixture of NaCl, Tris-HCl, phenylmethylsulfonyl fluoride and N-methylmaleic acid. imine mixed solution, wherein: the concen...

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Abstract

The invention discloses a preparation method of an artificial esophagus with histological structure. The method is characterized by comprising the steps of: extracting a basilemma membrane protein solution from animal esophageal mucosa tissue; then preparing electrospinning / acellular matrix bracket; then preparing a polyurethane sheet with a double-sided microgroove; and finally coating the polyurethane sheet on the electrospinning / acellular matrix bracket and splicing into a tube shape longitudinally, so as to obtain the artificial esophagus with histological structure. The invention has the advantage that the method can prepare the artificial esophagus simulating histological structure of normal human esophageal, and utilizes the principles of tissue engineering to construct in vitro the artificial esophagus with similar microstructure and function as human esophagus; and the artificial esophagus is seamed with residual esophagus after resection of lesion esophagus layer by layer, so that the artificial esophagus gradually grows into a real esophagus and integrates with the original esophagus in the human body. Compared with the traditional alternatives, the artificial esophagus has structure and function similar to those of natural esophagus, and can alleviate the suffering of patients and prolong the patient's life.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a method for preparing an artificial esophagus with histological structure. Background technique [0002] The normal human esophagus is a hollow tube about 20-25 cm long and 1.9 cm in diameter (varies from individual to individual), connecting the throat and stomach, and is responsible for transporting food from the throat to the stomach. From a histological point of view, the esophagus is composed of four layers: mucosa, submucosa, muscular layer, and adventitia, among which the mucosa and muscular layer are the most important functional components. For example, the mucosa is a permeable barrier layer that protects and isolates the internal tissues of the esophagus; the submucosa contains tubular glands that keep the surface of the mucosa lubricated to reduce friction when food passes through, so that food can pass without damaging the esophagus. The muscular layer contains o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/22A61L27/26A61L27/16
Inventor 竺亚斌侯雷吕静静巩长凤金嘉长
Owner NINGBO UNIV
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