Camellia nitidissima tea active site, preparation method and application thereof
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A technology of golden flower tea leaves and active parts, which is applied in the directions of food preparation, application, pharmaceutical formulation, etc.
Inactive Publication Date: 2014-09-10
GUANGXI MEDICAL UNIVERSITY
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[0018] One embodiment of the present application provides a method for preparing the active fraction of Camellia japonica, which includes the following steps:
[0019] A. Infiltration: take the coarse powder of golden camellia tea, soak overnight with 5 times (V / W) alcohol solution of 30% or more concentration;
[0020] B. Extraction: use the alcoholic solution of the same concentration as in step A to heat and reflux the golden camellia tea coarse powder in A step to extract 2 times or more than 2 times, and the alcohol solution consumption is 10~40 times of golden camellia coarse powder (V / W), each extraction time is 1 to 4 hours, filter, combine the extracts, reclaim the solvent to obtain a crude extract, filter, and take the filtrate to obtain a medicinal solution with a specific gravity of 1.05 to 1.25;
[0021] C. macroporous adsorption resin treatment: the concentrated paste is suspended in water, separated by macroporous resin column chromatography, and eluted with 10...
Embodiment 1
[0034] The preparation of the active part of embodiment 1 golden camellia
[0035] Golden camellia tea coarse powder 1000g, soaked 5 times in 70% ethanol overnight, heated and refluxed for 3 times, 3 hours for the first time, 2.5 hours for the second and third times respectively, combined the extracts, recovered the solvent, obtained the crude extract, filtered, and took The filtrate, the specific gravity of the medicinal solution is 1.05 to 1.25, and the obtained filtrate is separated by D101 macroporous resin column chromatography, and eluted with 10%, 30%, 80%, and 95% ethanol water of 10 column volumes successively, and the eluents are collected respectively. The 80% ethanol water eluted part was used to recover the solvent, freeze-dried to obtain the 80% ethanol part.
Embodiment 2
[0036] Embodiment 2 pharmacological activity test
[0037] 1) Reagent
[0038] a-glucosidase (a-glucosidase, EC3.2.1.20), 4-nitrophenyl-a-D-glucopyranoside (4-Ntrophenyl-a-D-glucopyranoside, PNPG, 026K1516), acarbose (Acarbose, Lot16869 , positive control) and DMSO were purchased from Sigma, and bovine serum albumin (5 components) (BSA) was purchased from Solarbio Co., Ltd.
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Abstract
The invention discloses a Camellia nitidissima tea active site, a preparation method and application thereof. The preparation method includes: A. infiltration: taking coarse Camellia nitidissima tea powder, and soaking the powder overnight with a quintuple (V/W) alcoholic solution with a concentration of 30% or above; B. extraction: employing an alcoholic solution with a same concentration to that of step A to conduct heating reflux extraction on the coarse Camellia nitidissima tea powder in step A twice or more, with the dosage of the alcoholic solution being 10-40 times (V/W) that of the coarse Camellia nitidissima tea powder, controlling the extraction time at 1-4h each time, performing filtration, merging the extracted liquid, conducting pressure reduced concentration, recovering the solvent to obtain a crude extracted liquid, conducting filtering and taking the filtrate to obtain a liquid medicine with a proportion of 1.05-1.25; C. treatment by macroporous adsorption resin: taking the filtrate obtained in step B, performing macroporous resin column chromatography separation, carrying out elution with 10%, 30%, 80% and 95% ethanol solution with a decuple column volume in order, collecting all the elution parts respectively, and conducting pressure reduced distillation on the 80% ethanol water elution part, with the macroporous resin selected from AB-8, D101, D201, D140, D141A, D301, D401, HP20 and HPD-100; and D. drying: freeze drying the 80% ethanol part obtained in step C.
Description
technical field [0001] The present invention relates to active part of traditional Chinese medicine, its preparation method and application. Background technique [0002] α-glucosidase plays an important role in the absorption process of food. Food must be combined with this enzyme to be digested and absorbed to increase blood sugar. α-Glucosidase inhibitors are a new type of oral hypoglycemic drugs widely used at present. Acarbose, voglibose and miglitol are commonly used in clinical practice. Inhibit the a-glucosidase of the brush border of the mesentery, delay the decomposition of polysaccharides (such as starch, oligosaccharides, etc.) into glucose by a-glucosidase, thereby slowing down the absorption rate of glucose and reducing postprandial blood sugar. Acarbose mainly inhibits a-amylase and acts on the digestion process of macromolecular polysaccharides; voglibose and miglitol selectively inhibit disaccharide hydrolase (maltase, sucrase) and decompose disaccharides ...
Claims
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