Magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules

A technology of biomolecules and recognition methods, applied in the field of biomolecular recognition, can solve problems such as large volume, high price, cross-activity interference, etc.

Active Publication Date: 2014-09-10
NANJING YIDEGUAN ELECTRONICS TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Similarly, the excitation of fluorescent molecules by light may produce a wide spectrum due to the presence of impurities and background, causing cross-activity interference
Finally, laser scanning systems for fluorescence detection are usually bulky, slow and expensive, and cannot meet the needs of community medical care, rural primary medical care and families for rapid early diagnosis of major diseases and large-scale screening of food safety

Method used

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  • Magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules
  • Magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules
  • Magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Medical application: detection of breast cancer marker molecule CEA

[0050] The schematic diagram of the whole identification method can be as follows figure 1 , which shows the connection modification methods before each device, antibody, and antigen; the whole step process, such as figure 2 shown;

[0051] Step 1 Synthesis and surface modification of magnetic nanoparticles:

[0052] In order to improve the chemical stability and biocompatibility of magnetic particles, considering the three-dimensional space structure, hydrogen bond, electrostatic force and hydrophobic effect, the present invention adopts wrapping inert metal, high molecular polymer and silica shell on the surface of magnetic nanoparticles Direct surface modification technology enables the surface of magnetic nanoparticles to have chemical stability and excellent biocompatibility. In this example, Fe 3 o 4 Taking magnetic particles as an example, the specific method is to weigh 1.2 g...

Embodiment 2

[0073] Example 2: Food safety application: detection of aflatoxin (AFB1) content in rice

[0074] Synthesis and surface modification of the first step of magnetic nanoparticles: the same as the first step of Example 1.

[0075] Step 2, surface biofunctionalization of magnetic particles: same as Step 2 of Example 1.

[0076] Step 3, fabrication of the magnetic quantum sensor array: the same as the third step of Embodiment 1, but the size of the sensor array is 50x50 microns (μm).

[0077] Step 4, surface modification of the magnetic quantum sensor: same as Step 4 of Example 1.

[0078] Step 5, the chemical method of binding the capture antibody to the sensor surface: use a pipette to drop 50nL of the antibody to the AFB1 antigen on the sensor surface (purchased from Sigma–Aldrich, St. Louis, MO, the United States), and place on the control sensor 50mL of bovine serum albumin (bovine serum albumin, BSA) was dripped into part with a pipette. The sensor chip was then left at 4°...

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Abstract

The invention relates to a magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules, and belongs to the technical field of biomolecule identification. The invention mainly aims at an immunolabelling biomolecule detection, monitoring and identification method based on systems such as antigens-antibodies, cell factors-cell factor acceptors, bioactive peptides-acceptors, biotin-avidin and chiral molecules. A biomolecule probe and detection system consists of superparamagnetic particles and high-performance magnetic sensors. The magnetic sensing identification method can be applied to the application fields of biology, medicines, food safety and the like, and can be used for biomolecule identification, detection and monitoring, clinical disease diagnose, food safety detection and virus and germ detection.

Description

technical field [0001] The invention relates to a high-throughput, multi-channel and low-abundance biomolecular magnetic sensing recognition method, which belongs to the technical field of biomolecular recognition. It can be used in the fields of biology, medicine and food safety, and can be used for biomolecular identification, detection and monitoring; clinical disease diagnosis; food safety detection; virus and bacteria detection. Background technique [0002] Biomolecular recognition has a wide range of applications in RNA strand recognition, gene detection, bacterial diagnosis, new drug discovery, DNA defects and lethal agents in biological warfare, food safety, and early diagnosis and detection of major diseases represented by malignant tumors. With the completion of the human gene map, it has become possible to understand a large number of unknown gene chain information in the past. Biomolecular recognition and detection has expanded from the field of scientific re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N27/72
CPCG01N27/72G01N33/53G01N33/54346
Inventor 章伟胡雪峰
Owner NANJING YIDEGUAN ELECTRONICS TECH
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