Kit and method for quickly extracting DNA (deoxyribonucleic acid) from colla corii asini

Abstract

The invention discloses a kit and a method for quickly extracting DNA (deoxyribonucleic acid) from colla corii asini. A combined technology of an SDS (sodium dodecyl sulfate)-protease K and Chelex-100 combined process DNA extraction technology and a Glassmilk DNA purification technology is applied to DNA extraction of the colla corii asini. The dosage of needed colla corii asini paste is controlled to be 0.3ml to 0.5ml, the weight of colla corii asini blocks can be controlled to be 0.2mg to 0.6mg, and extracted DNA is high in concentration, excellent in purity, stable and difficult to degrade; due to extracted DNA, sequence fragments of 100bp-500bp can be amplified, and subsequent molecular biology experiments of gene cloning, sequencing and the like can be carried out. According to the method, the operation is simple, the dosage is low, and the consumed time is short; the method is especially suitable for DNA extraction of various types of colla corii asini products with low nucleic acid content and high degradation degree caused by deep processing; a foundation is provided for realizing colla corii asini source identification by adopting a DNA molecular biology identification technology.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for rapidly and efficiently extracting DNA from donkey-hide gelatin and a kit thereof. Background technique [0002] Donkey-hide gelatin is a jelly made from the dehaired skin of an equine animal donkey. It has a wide range of medical and health care values. Therefore donkey-hide gelatin occupies an important position in the market as a kind of health food, and is deeply favored by consumers. However, with the continuous development of the gelatin market, the shortage of raw material supply and the rise in raw material prices, in order to reduce costs, lawbreakers, in the process of preparing donkey-hide gelatin, partly or completely mix horsehide, cowhide, pigskin, mule The "fake donkey-hide gelatin" made in this way is very similar to the real donkey-hide gelatin in appearance and texture, and the price is low. The existence of such counterfeit products not...

AI Technical Summary

Problems solved by technology

[0003] The traditional method of identifying donkey-hide gelatin is mainly realized by observing the product appearance, color, smell, etc. However, it is difficult to distinguish the authenticity of donkey-hide gelatin boiled from mule and horse hide, which are closely homologous to donkey hide

In recent years, with the development of molecular biology technology, some biological identification methods based on DNA have been gradually enriched. The patent publication number CN1605868A uses PCR-RFLP to identify animal skins such as donkey skins, but this method is only applicable to animal skins and not It is suitable for the identification of finished donkey-hide gelatin. After being exposed to high temperature and high pressure for a long time, the DNA content of donkey-hide gelatin is very small and has been degraded into small fragments. It is difficult to amplify long-segment PCR products.

The patent with the publication number CN101270357A provides a method for extracting DNA from deep-processed traditional Chinese medicine or traditional Chinese medicinal materials, but this method requires a large amount of raw materials in a 50ml centrifuge tube, which is time-consuming and laborious, and must be extracted using imported commercial kits, resulting in high experimental costs

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