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Method for preparing chitosan through composite fermentation

A technology of chitosan and compound fermentation, applied in the field of manufacturing, can solve the problems of low activity of chitin, achieve the effects of low energy consumption, avoid resource waste, and reduce the amount of strong acid

Inactive Publication Date: 2014-09-17
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main function of CDA derived from fungi is the synthesis of its own cell wall. The most suitable substrate is generally chitosan oligosaccharides, which have low activity on chitin and are not suitable for the production of chitosan by enzymatic deacetylation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of chitin deacetylase:

[0028] (1) Preparation of fermentation medium: yeast powder 8g, chitosan 15g, glucose 30g, potassium dihydrogen phosphate 2g, anhydrous magnesium sulfate 1g, cobalt dichloride (0.01mol / l) 10ml, water 1000ml;

[0029] (2) Fermentation conditions: sterilization of the fermentation medium, inoculation of Aspergillus nidulans, 30°C, pH 6.0, 72h.

[0030] Dry and crush crab or shrimp shells to 0.5mm particles, add 4 times the weight-to-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria accounted for The weight ratio of raw materials is 0.02%, fermented for 24 hours, then 70°C for 20 minutes; the fermentation broth is filtered, the residue is collected, and the filtrate is recycled and dried to produce bio-organic calcium. Add 8% hydrochloric acid to the residue for decalcification, after fully reacting, filter, wash...

Embodiment 2

[0032] Preparation of chitin deacetylase:

[0033] (1) Preparation of fermentation medium: yeast powder 8g, chitosan 15g, glucose 30g, potassium dihydrogen phosphate 2g, anhydrous magnesium sulfate 1g, cobalt dichloride (0.01mol / l) 10ml, water 1000ml;

[0034] (2) Fermentation conditions: sterilization of the fermentation medium, inoculation of Aspergillus nidulans, 35°C, pH 7.0, 96h.

[0035] Dry and crush crab or shrimp shells to 1.0mm particles, add 8 times the weight-to-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria account for The weight ratio of raw materials is 0.06%, fermented for 36 hours, and then 100°C for 20 minutes; the fermentation broth is filtered, the residue is collected, and the filtrate is recovered and dried to produce bio-organic calcium. Add 15% hydrochloric acid to the residue for decalcification, after fully reacting, filter,...

Embodiment 3

[0037] Preparation of chitin deacetylase:

[0038] (1) Preparation of fermentation medium: yeast powder 8g, chitosan 15g, glucose 30g, potassium dihydrogen phosphate 2g, anhydrous magnesium sulfate 1g, cobalt dichloride (0.01mol / l) 10ml, water 1000ml;

[0039] (2) Fermentation conditions: sterilization of the fermentation medium, inoculation of Aspergillus nidulans, 34°C, pH 6.5, 82h.

[0040] Dry and crush the crab or shrimp shells to 0.5mm particles, add 8 times the weight-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria accounted for The weight ratio of raw materials is 0.04%, fermented for 36 hours, then 80°C for 20 minutes; the fermentation broth is filtered, the residue is collected, and the filtrate is recycled and dried to produce bio-organic calcium. Add 10% hydrochloric acid to the residue for decalcification, after fully reacting, filter, wa...

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PUM

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Abstract

The invention discloses a method for preparing chitosan through composite fermentation. The method comprises the following steps: drying crab shell or shrimp shell, and crushing; fermenting; removing calcium; removing proteins; and carrying out enzymatic hydrolysis for acetyl removal, washing with water, drying, and crushing. Compared with the prior art, the above enzymatic hydrolysis method has the advantages of reduction of the consumption of a strong acid, low energy consumption, no pollution and environmental protection. A fermentation liquid can be recovered to prepare biological osteocalcin, so all products can be reasonably utilized to generate substantial economic values and avoid resource waste.

Description

technical field [0001] The invention relates to a preparation method of chitosan and belongs to the field of manufacturing. Background technique [0002] Chitin is a natural nitrogen-containing polysaccharide substance, which widely exists in the shell or cuticle of invertebrates, especially in the shells of shrimp and crabs. Chitin is easy to obtain and renewable. It is a natural homopolymer second only to cellulose. However, due to the strong hydrogen bond in the molecule, the solubility is low and the application range is limited. [0003] Chitosan is formed after deacetylation of chitin. At present, chitosan is usually prepared by chemical and enzymatic deacetylation. In the chemical deacetylation reaction, there are many factors affecting the deacetylation, but the main influencing factors include: lye concentration, reaction temperature and time. In general, increasing the reaction temperature and prolonging the reaction time can increase the degree of deacetylatio...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12R1/66
Inventor 杨国元白洁苗胜昆李莹李雨田许卉婷陶会会张原
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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