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A method for preparing fuel ethanol by carbon-rich microalgae solid acid saccharification and fermentation

A technology of saccharification and fermentation, fuel ethanol, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of occupying large arable land and rising food prices, and achieve the effect of rapid growth

Active Publication Date: 2016-08-17
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the bioethanol production around the world uses food crops as raw materials, which requires a large amount of arable land, and the consumption of food will stimulate food prices, bringing many environmental and economic problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Put 200g (dry weight: 40g) of culture solution of chlorella algae mud that has been concentrated by centrifugation into a high-temperature liquid water treatment device, add water to a volume of 400ml, and add 2g of solid acid; the temperature of the reaction kettle is 120°C for 80 minutes of hydrolysis.

[0021] (2) Filtrate and reclaim the solid acid, process the filtered microalgae liquid with resin adsorption to remove furfural and other substances in the microalgae liquid; add inorganic salt substances to the microalgae liquid after adsorption treatment, Add 0.5g (NH4) 2 HPO 4 , 0.025g MgSO 4 ·7H 2 O, 1.0g yeast extract, then add Na 2 HPO 4 - citric acid buffer, adjusting the pH of the reaction system to 4.5-5.5.

[0022] (3) Add 40 FPU / g of amylase and cellulase to the reaction system, and pre-hydrolyze at 50° C. for 24 hours.

[0023] (4) Saccharomyces cerevisiae NRRL Y-2034 was purchased from the Northern Regional Research Laboratory of the United Stat...

Embodiment 2

[0026] (1) Put 30 g of chlorella dry powder culture solution in a high-temperature liquid water treatment device, add water to 600 ml, and add 3 g of solid acid. The reaction kettle temperature is 140°C for 60 minutes of hydrolysis.

[0027] (2) Filtrate and reclaim the solid acid, process the filtered microalgae liquid with resin adsorption to remove furfural and other substances in the microalgae liquid; add inorganic salt substances to the microalgae liquid after adsorption treatment, Add 0.5g (NH4) 2 HPO 4 , 0.025g MgSO 4 ·7H 2 O, 1.0g yeast extract, then add Na 2 HPO 4 - citric acid buffer, adjusting the pH of the reaction system to 4.5-5.5.

[0028] (3) Add amylase and cellulase 30 FPU / g substrate enzyme amount respectively to the reaction system, and pre-hydrolyze at 50° C. for 18 hours.

[0029] (4) Saccharomyces cerevisiae (Saccharomyces cerevisiae) NRRL Y-2034 was purchased from the Northern Regional Research Laboratory of the United States Department of Agric...

Embodiment 3

[0032] (1) Place 50 g of Nannochloropsis dry powder culture solution concentrated by centrifugation into a high-temperature liquid water treatment device, add 500 ml of water, and add 5 g of solid acid. Reactor temperature is 100°C for 120 minutes of hydrolysis;

[0033] (2) Filtrate and reclaim the solid acid, process the filtered microalgae liquid with resin adsorption to remove furfural and other substances in the microalgae liquid; add inorganic salt substances to the microalgae liquid after adsorption treatment, Add 0.5g (NH4) 2 HPO 4 , 0.025g MgSO 4 ·7H2 O, 1.0g yeast extract, then add Na 2 HPO 4 - citric acid buffer, adjusting the pH of the reaction system to 4.5-5.5.

[0034] (3) Add amylase and cellulase 20 FPU / g substrate enzyme respectively to the reaction system, and pre-hydrolyze at 50° C. for 24 hours.

[0035] (4) Saccharomyces cerevisiae NRRL Y-2034 was purchased from the Northern Regional Research Laboratory of the United States Department of Agriculture...

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Abstract

The invention provides a method for preparing fuel ethanol by saccharifying and fermenting carbon-rich microalgae solid acid. The method comprises the following steps: by taking microalgae rich in carbohydrate as a raw material, processing a centrifugally-concentrated microalgae culture solution by using high-temperature liquid water in the presence of a catalyst which is solid acid, and subsequently fermenting to prepare ethanol. The method is simple and environment-friendly; the microalgae is available and quick in growth; moreover, cellulose hydrogen bonds in the microalgae cells are relatively weak and more likely to be degraded into monosaccharide; and due to the preparation of the ethanol by using the microalgae, the problem of high cost due to the preparation of the ethanol by using maize and wheat and the like as the raw materials in the past is solved.

Description

technical field [0001] The invention relates to the technical field of bioenergy preparation, in particular to a process method for preparing ethanol fuel through saccharification and fermentation of microalgae. Background technique [0002] Renewable bioenergy is becoming more and more important in the current energy field, and bioethanol occupies a pivotal position. Nowadays, the price of fossil liquid fuels continues to rise. All countries in the world need bioethanol to supplement the energy gap. Researchers are also committed to Development of a large-scale production process for bioethanol. At present, the United States and Brazil, the two largest producers of bioethanol in the world, both use food crops as raw materials. Most of the bioethanol production around the world uses food crops as raw materials, which requires a large amount of arable land, and the consumption of food will stimulate food prices and bring many environmental and economic problems. The second ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14C12P19/02C12R1/865
CPCY02E50/10
Inventor 袁振宏周卫征王忠铭许敬亮张宇郭颖梁翠谊
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI