Construction and application of chemiluminiscence-colloidal gold immunochromatography test paper
An immunochromatographic test paper and chemiluminescent technology, which is applied in scientific instruments, analytical materials, biological testing, etc., can solve the problems of low detection sensitivity, long time-consuming, complicated detection steps, etc., and achieve large specific surface area, high sensitivity, and optical properties. big effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0058] figure 1 and figure 2 They are the schematic diagram of the immunochromatography test strip and the construction principle diagram of the chemiluminescence-colloidal gold immunochromatography test strip, respectively. The specific steps for the construction of the chemiluminescent-colloidal gold immunochromatographic test strip are as follows:
[0059] 1) Preparation of colloidal gold-enzyme-antibody complex:
[0060] Adjust the pH value of 10mL of colloidal gold solution to 8.5, then add 0.01mg of detection antibody and 0.2mg of horseradish peroxidase (HRP), the coupling ratio of the enzyme and antibody is 10:1, at room temperature Gently shake and react for 1-2 hours, then add 3mL of 3% BSA solution to block the excess sites, react for 0.5-1 hour, then centrifuge for 10-20 minutes at a centrifugal force of 10000-12000r / min, remove the upper Add 2 mL of PBS solution containing 0.1% BSA with a concentration of 0.01M and a pH value of 7.4, resuspend, and then centrif...
Embodiment 2
[0066] Detection of alpha-fetoprotein (AFP).
[0067] The experimental steps are as follows:
[0068] (1) Use PBS buffer solution to construct AFP solutions with concentrations of 200ng / mL, 100ng / mL, 50ng / mL, 20ng / mL, 10ng / mL, 5ng / mL, 1ng / mL and 0ng / mL, and then add them to Chemiluminescence-colloidal gold immunochromatography test strips, immunochromatography reaction 5-10min;
[0069] (2) Observe the color depth of the colloidal gold on the test strip with the naked eye, and take pictures with a camera to obtain the result of visual detection of AFP;
[0070] (3) Add a chemiluminescent substrate to the original test strip, and quantitatively detect the result with a chemiluminescent instrument.
[0071] image 3 It is the result of visual detection of alpha-fetoprotein (AFP) by chemiluminescence-colloidal gold test strips, from image 3 It can be seen from the color of the colloidal gold that the minimum detection limit of the test strip for AFP is 10ng / mL.
[0072] F...
Embodiment 3
[0075] Detection of fetal cancer protein (CEA) in serum.
[0076] The experimental steps are as follows:
[0077] (1) Use PBS buffer solution to construct CEA solutions with concentrations of 200ng / mL, 100ng / mL, 50ng / mL, 20ng / mL, 10ng / mL, 5ng / mL, 1ng / mL and 0ng / mL, and then add them to Chemiluminescence-colloidal gold test strips, immunochromatographic reaction for 5-10min;
[0078] (2) Observe the color depth of the colloidal gold on the test strip with the naked eye, and take pictures with a camera to obtain the result of visual detection of CEA;
[0079] (3) Add a chemiluminescent substrate to the original test strip, and quantitatively detect the result with a chemiluminescent instrument.
[0080] Figure 5 It is the result of visual detection of Fetal Oncoprotein (CEA) by chemiluminescence-colloidal gold test strips, from Figure 5 It can be seen from the color of the colloidal gold that the minimum detection limit of the test strip for CEA is 10 ng / mL.
[0081] Fi...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com