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An immobilized fiber bed reactor for fermentative production of 1,3-propanediol

A reactor and fiber bed technology, which is applied in the field of 1,3-propanediol production equipment, achieves the effects of low cell immobilization cost, improved utilization rate, and improved mass transfer efficiency

Active Publication Date: 2016-10-12
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the production of 1,3-propanediol by fiber bed reactor

Method used

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  • An immobilized fiber bed reactor for fermentative production of 1,3-propanediol
  • An immobilized fiber bed reactor for fermentative production of 1,3-propanediol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Clostridium butyricum Gen 160 was used to produce 1,3-propanediol in an immobilized fiber bed reactor.

[0054] Seed medium (g·L -1 ): Glycerin, 20; K 2 HPO 4 ·3H 2 O, 4.45; KH 2 PO 4 , 1.3; (NH 4 ) 2 SO 4 , 2; MgSO 4 ·7H 2 O, 0.2; CaCl 2 ·2H 2 O, 0.02; Yeast powder, 1. Trace element solution 1mL, Fe solution 1mL.

[0055] Fermentation medium (g·L -1 ): Glycerin, 50; K 2 HPO 4 ·3H 2 O, 1; KH 2 PO 4 , 0.5; (NH 4 ) 2 SO 4 , 2; MgSO 4 ·7H 2 O, 0.2; CaCl 2 ·2H 2 O, 0.02; Yeast powder, 1. 1 mL of trace element solution; 1 mL of Fe solution; pH 6.5.

[0056] Fe solution: FeSO 4 ·7H 2 O, 5g·L -1 ; HCl(37%), 4ml·L -1 .

[0057] Trace element solution (g·L -1 ): ZnCl 2 , 0.07; MnCl 2 ·4H 2 O, 0.1; H 3 BO 3 , 0.06; CoCl 2 ·6H 2 O, 0.2; CuCl 2 ·2H 2 O, 0.02; NiCl 2 ·6H 2 O, 0.025; Na 2 MoO 4 ·2H 2 O, 0.035; HCl (37%), 0.9 mL·L -1 .

[0058] Connect the Clostridium butyricum Gen 160 seed liquid to a fermentor equipped with 2L fermentation medium at an inoculum of 10% (v / v), at a constant t...

Embodiment 2

[0060] Clostridium butyricum Gen 160 was used to produce 1,3-propanediol in an immobilized fiber bed reactor.

[0061] The medium is the same as in Example 1.

[0062] Connect the Clostridium butyricum Gen 160 seed liquid to a fermentor equipped with 2L fermentation medium at an inoculum of 10% (v / v), at a constant temperature of 37°C, a rotation speed of 150 rpm, and a pH of 7.0 controlled, and pass through the gas inlet 0.1L / min N 2 , Fermentation for 8h to logarithmic growth phase. The lye bottle is filled with 4mol / L NaOH solution. The reactor is pre-passed with N 2 After deoxygenation, 8 hours later, the fermentation broth in the fermentor was filled into the reactor at a flow rate of 40 mL / min with a peristaltic pump to fix the bacteria on the synthetic fiber material. Add 2L of fermentation medium with 800g glycerin content to the fermenter, and use a peristaltic pump to dilute the medium to 0.1h -1 Fill the reactor with fermentation medium, use a peristaltic pump to feed ...

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Abstract

The invention provides an immobilized fibrous-bed reactor for producing 1,3-propylene glycol by fermentation, and relates to 1,3-propylene glycol. The immobilized fibrous-bed reactor is provided with a reactor body, a fermentation tank and an automatic pH control device, wherein the automatic pH control device is provided with a fermentation tank pH probe, a reactor pH probe, a fermentation tank pH detector, a reactor pH detector, alkali liquor peristaltic pumps, alkali liquor bottles and air filters; an alkali liquor outlet of the first alkali liquor bottle is connected to the fermentation tank by virtue of the first alkali liquor peristaltic pump; the fermentation tank pH probe is connected with the fermentation tank, the output end of the fermentation tank pH probe is connected with the fermentation tank pH detector, and an outlet of the fermentation tank air filter is connected with the fermentation tank; an outlet of the fermentation tank is connected with the reactor by virtue of a fermentation broth peristaltic pump; an outlet of the second alkali liquor bottle is connected with the reactor by virtue of a peristaltic pump, and the air filter is arranged on the second alkali liquor bottle; the reactor pH probe is connected with the reactor, and a fiber material is filled in the reactor; the reactor pH probe is connected with the reactor pH detector; and a sieve plate is arranged at the bottom in the reactor; a tail gas outlet is formed in the reactor, and the reactor is connected with a product collection device.

Description

Technical field [0001] The invention relates to 1,3-propanediol production equipment, in particular to an immobilized fiber bed reactor for fermentative production of 1,3-propanediol. Background technique [0002] 1,3-propanediol (1,3-propanediol, abbreviation: 1,3-PD) is an important chemical raw material. Because it contains bifunctional groups, it can participate in a variety of chemical synthesis reactions. For example, it can be used as a monomer to produce polycondensates such as polyester, polyether, and polyurethane for special occasions; as a raw material for organic synthesis intermediates, it can be used in a variety of Synthesis of drugs and new antioxidants; as a solvent, it can improve the excellent performance of adhesives, resins, detergents, cosmetics and other products (Kaur G, Srivastava AK, Chand S. Advances in biotechnological production of 1,3-propanediol[J] .Biochemical Engineering Journal,2012,64:106-118). In addition, using 1,3-propanediol instead of 1,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/36C12M1/34C12M1/04
CPCC12M21/16C12M25/18C12M29/18C12M41/26
Inventor 方柏山邱隆辉张蓝蓝王世珍
Owner XIAMEN UNIV