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Strain of yeast capable of decomposing cellulose and applications thereof

A cellulose and yeast technology, applied in the field of yeast, can solve the problem of less yeast, and achieve the effects of short production cycle, good adaptability and simple production method

Active Publication Date: 2014-11-05
YIBIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, there have been few studies on the yeast that degrades cellulose, and the yeast that can directly degrade distiller's grain cellulose without any treatment has not been reported.

Method used

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  • Strain of yeast capable of decomposing cellulose and applications thereof
  • Strain of yeast capable of decomposing cellulose and applications thereof

Examples

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Effect test

Embodiment 1

[0022] Embodiment 1 Utilizes yeast of the present invention to produce high-protein feed with cellulose as the main raw material

[0023] First, inoculate 1 loop of the yeast strains preserved on the slant into YPD liquid medium with an inoculation needle, and cultivate at 32°C for 1 day, then centrifuge the obtained yeast suspension at 3000rpm for 5min at 25°C, and pour off the supernatant. After adding sterile water, mix well and centrifuge again under the same conditions, wash the bacteria, and make a yeast suspension with a concentration of 0.5OD;

[0024] According to the inoculum amount of 4%, use the sprayer to directly spray the yeast suspension into the fresh distiller's grains, mix well, pile up in the open air for 2-3 days, monitor the change of acidity, and detect the protein content of the distiller's grains until the pH rises to about 5, reaching 16- 18% can be processed into high-protein grain powder feed according to the conventional grain powder feed processin...

Embodiment 2

[0025] Example 2 Utilize the yeast of the present invention to produce distilled grains wine, improve the yield and quality of distilled grains wine

[0026] Yeast suspension was prepared according to the method in Example 1. While mixing koji powder, it was inoculated into the distiller's grains fermented with Daqu complex grains by 1% inoculation amount, and fermented in the cellar to produce distiller's grains wine. Compared with fermented distilled grains fermented with yeast, it can increase the yield of distilled grains wine by 0.8-1%, while not affecting other physical and chemical indicators of distilled grains wine.

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Abstract

The invention discloses a strain of yeast capable of decomposing cellulose and applications thereof, and relates to yeast having characteristic physiological functions. The yeast is separated from a producing area of Luzhou-flavor liquor in Yibin. The preservation number of the yeast is CGMCC No.9140. The yeast is named as Debaryomyces hansenii. The growth temperature is 27 to 34 DEG C, the growth pH value is 4.5 to 6.5, the bacterium colony is white after being cultured in a YPD culture medium for 2 days at a temperature of 32 DEG C, the diameter is around 5 mm, the surface is wet, smooth, and projected, the bacterium colony is easy to pick up, and the cell is in a round shaped, and is produced through gemmation. After the yeast is cultured in a YPD liquid culture medium with a pH value of 5.8 for 36 hours at a temperature of 32 DEG C, the activity of the cellulose produced by the yeast is 1.837 U / mL. The yeast can be used to produce high protein feed mainly composed of cellulose, and the protein content of white vinasse can be increased by 60% or more. The yeast can also be used to produce vinasse-free liquor, and the quality and output of the vinasse-free liquor are both improved.

Description

technical field [0001] The invention relates to a yeast with characteristic physiological functions, in particular to a strain of cellulose-decomposing yeast and its application. Background technique [0002] At present, in addition to physical and chemical methods such as steam explosion and acid hydrolysis, the decomposition of cellulose mainly uses the cellulase produced by mold or bacteria to decompose, especially mold such as Trichoderma viride, which produces cellulase under aerobic fermentation The capacity can reach 30U / mL fermentation broth, and it is currently the main cellulase production strain. [0003] In production, the cellulase produced by mold is generally used directly, but distiller's grains of liquor have high acidity and are rich in various alcohols, aldehydes, esters and other substances, and the enzyme activity of cellulase produced by mold is easily affected by these alcohols in distiller's grains. , aldehyde and other harmful substances, resulting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12G3/02A23K1/00A23K1/06C12R1/645A23K10/12
CPCY02P60/87
Inventor 游玲王涛王松
Owner YIBIN UNIV
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