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Application of avian infectious bronchitis virus vaccine strain in preparation of inactivated vaccine

A bronchitis, chicken infectious technology, applied in the field of prevention and treatment of chicken infectious bronchitis, can solve the problems such as unsatisfactory control effect

Inactive Publication Date: 2014-11-05
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the existing chicken infectious bronchitis inactivated vaccine strain M41 (viral spike protein (S1) genetic evolution relationship is similar to Massachusetts, those skilled in the art will be summarized as Mass type chicken infectivity bronchitis strain) can not effectively prevent the attack of chicken infectious bronchitis (Avian Infectious Bronchitis, IB) epidemic strain, and the defects such as the control effect of IB are not ideal, provide a strain of new chicken infectious bronchitis vaccine strain ( Virus spike protein (S1) is similar to LX4 strain or QX strain in genetic evolution relationship, and those skilled in the art will summarize it as LX4 type (QX-like) strain), and this vaccine strain can effectively resist the main epidemic infectious bronchitis in my country at present. Yanye strain attack can provide good immune protection effect

Method used

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  • Application of avian infectious bronchitis virus vaccine strain in preparation of inactivated vaccine
  • Application of avian infectious bronchitis virus vaccine strain in preparation of inactivated vaccine
  • Application of avian infectious bronchitis virus vaccine strain in preparation of inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Isolation and Identification of Chicken Infectious Bronchial Virus Strain ck / CH / LLN101135

[0022] 1. Materials and methods

[0023] 1.1 On-site samples and virus isolation: During our continuous epidemiological monitoring of IBV, kidney samples were collected from chicken flocks suspected of IB infection in various parts of my country. The affected chickens showed early clinical symptoms of respiratory diseases, including panting, Coughing, sneezing and tracheal rales. Necropsy of the dead chickens showed different degrees of tracheitis, nephritis and glandular gastritis, and the laying hens mainly showed death, decreased egg production and deformed eggs. The samples collected in this study were from chicken flocks immunized with Mass-type commercial attenuated vaccine.

[0024]When the virus is isolated, grind and suspend the sample in 0.1% PBS buffer, centrifuge at 1500g for 10min at 4°C, filter the supernatant through a 0.22μm filter membrane, and inocula...

Embodiment 2

[0049] Example 2 The immune efficacy experiment of the infectious bronchitis inactivated vaccine strain ck / CH / LLN101135 strain of the present invention

[0050] 1. Materials and methods

[0051] 1.1 Vaccines for basic immunization

[0052] Mass-type infectious bronchitis inactivated vaccine (H120 strain) was purchased from Harbin Veken Biotechnology Development Company. The storage conditions and validity period of the vaccine were: stored below -15°C, and the validity period was 12 months. According to the label of the bottle, the feather portion is diluted with sterilized physiological saline, and the vaccine is inhaled, and each chicken is inoculated with 1 portion by eye drops and nasal drops.

[0053] 1.2 Proliferation of virus LX4 type (QX-like) infectious bronchitis virus ck / CH / LLN101135 strain

[0054] Use 10-day-old SPF chicken embryos to dilute the seed virus of ck / CH / LLN101135 strain 10,000 times. Each embryo is inoculated through the allantoic cavity, and each em...

Embodiment 3

[0078] Example 3 Safety experiment of LX4 type (QX-like) infectious bronchitis inactivated vaccine (ck / CH / LLN101135) of the present invention

[0079] 1 Experimental materials

[0080] 1.1 Experimental vaccine:

[0081] According to the method of Example 2, 4 batches of vaccines were prepared, and the batch numbers of the 4 batches were: 2012001, 2012002, 2012003, 2012004, 500 feathers / bottle. Vaccine storage conditions and validity period: 2 ~ 8 ℃ storage, the validity period is 12 months. According to the label of the bottle, draw the vaccine with a sterilized syringe, and inoculate each chicken subcutaneously or intramuscularly.

[0082] 1.2 Experimental animals:

[0083] One-week-old SPF chickens were purchased from the Experimental Animal Center of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0084] 2 Experimental methods

[0085] 2.1 SPF chicken safety test

[0086] 2.1.1 Single-dose inoculation trial

[0087] 30 1-day-old and ...

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Abstract

The invention discloses an application of avian infectious bronchitis virus vaccine strain in the preparation of inactivated vaccine. The separated wild virulent strain of infectious bronchitis virus is subjected to chick embryo adaptability domestication so as to obtain an infectious bronchitis virus vaccine strain, which can well adapt to the chick embryo, maintains good hereditary characters and immunogenicity, and is named as ck / CH / LLN101135. Then the obtained infectious bronchitis virus vaccine strain is inactivated and subjected to immune efficacy evaluation experiments. The experiment results show that the infectious bronchitis inactivated vaccine prepared from the vaccine strain can trigger the organism to generate a good immune response, can prominently improve the motive immune protective effectiveness of chicken at the same time, and effectively protects chicken from being affected by infectious bronchitis virus. The infectious bronchitis virus vaccine strain can be used to prepare single vaccine or combined vaccine of avian infectious bronchitis.

Description

technical field [0001] The invention relates to a virus vaccine strain, in particular to a virus vaccine strain of chicken infectious bronchitis and its application in preparing an inactivated vaccine. The invention belongs to the field of prevention and treatment of chicken infectious bronchitis. Background technique [0002] Chicken infectious bronchitis (Avian Infectious Bronchitis, IB) is a kind of acute, highly contagious, viral respiratory disease caused by chicken infectious bronchitis virus (Infectious Bronchitis Virus, IBV), it is characterized in that: sick chicken coughs , sneezing, tracheal rales; enlarged, pale kidneys, dilated ureters, a large amount of urate deposition, and the appearance of a typical "mottled kidney". Chicken infectious bronchitis has been prevalent in countries all over the world since 1930, causing large economic losses. With the spread and genetic evolution of the virus, there are currently multiple serotypes of IBV. [0003] IBV is curr...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/215A61P31/14C12R1/93
Inventor 刘胜旺韩宗玺邵昱昊孔宪刚
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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