Functional white kidney bean polypeptide and preparation method and application thereof
A functional polypeptide, white kidney bean technology, applied in the preparation method of peptide, chemical instrument and method, peptide and other directions, can solve the problems of low thermal stability of α-amylase inhibitor and unfavorable industrial production and application, etc.
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Embodiment 1
[0018] Take 1000g of white kidney bean powder as raw material, add 10L of distilled water, adjust the pH to 5.0 with citric acid, stir and extract in a water bath at 45°C for 5h, then centrifuge at 3500rpm for 15min and collect the supernatant to be white kidney bean albumin. The albumin was ultrafiltered with a 10000Da ultrafiltration membrane and the retentate part was collected. Adjust the pH of the retentate to 3.0, add 0.1g of 537 acid protease per gram of protein, enzymolyze in a water bath at 45°C for 0.5, 1, 1.5, 2 and 2.5 hours, then inactivate the enzyme in a water bath, cool and centrifuge to get the supernatant , which is the white kidney bean polypeptide. The α-amylase inhibitory activity of white kidney bean polypeptides with different enzymatic hydrolysis time was determined by 3,5-dinitrosalicylic acid colorimetric method. High, see Table 2.
[0019] The polypeptide solution is ultrafiltered with a 6000Da ultrafiltration membrane to collect the retentate, and...
Embodiment 2
[0027] Take 1000g of white kidney bean powder as raw material, add 15L of distilled water, adjust the pH to 4.5 with citric acid, stir and extract in a water bath at 50°C for 4 hours, then centrifuge for 15 minutes and collect the supernatant to be white kidney bean albumin. The albumin was ultrafiltered with a 10000Da ultrafiltration membrane and the retentate part was collected. Adjust the pH of the retentate to 4.0, add 0.15g of 537 acid protease per gram of protein, enzymatically hydrolyze in a water bath at 40°C for 1 hour, then inactivate the enzyme at 80°C for 10 minutes, and centrifuge after cooling to obtain the supernatant, which is the white kidney bean polypeptide. The polypeptide solution is ultrafiltered with a 6000Da ultrafiltration membrane to collect the retentate, and then the retentate is concentrated to a water content of 50%, and isomaltulose is added in a mass ratio of 75:25, stirred evenly, and then spray-dried to obtain white Kidney bean peptide product...
Embodiment 3
[0034] Take 1000g of white kidney bean powder as raw material, add 18L of distilled water, adjust the pH to 5.3 with citric acid, stir and extract in a water bath at 55°C for 3h, then centrifuge at 3500rpm for 15min to collect albumin. The albumin was ultrafiltered with a 10000Da ultrafiltration membrane and the retentate part was collected. Adjust the pH of the retentate to 2.8, add 0.2g of 3.350 acid protease per gram of protein, enzymatically hydrolyze in a water bath at 50°C for 0.6h, then inactivate the enzyme at 80°C, cool and centrifuge, and the supernatant is the white kidney bean polypeptide. The polypeptide solution is ultrafiltered with a 6000Da ultrafiltration membrane to collect the retentate, and then the retentate is concentrated to a water content of 50%, and isomaltulose is added in a ratio of 84:16 by mass, stirred evenly, and vacuum-dried to obtain white Kidney bean peptide products.
[0035] Take Kunming mice and divide them into basic group, model group, ...
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