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A method for continuously treating molasses alcohol wastewater to produce pha with halophilic mixed bacteria

A technology of molasses alcohol wastewater and halophilic mixed bacteria, applied in the direction of sustainable biological treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problem of large manpower, material and power consumption, short effective fermentation time, and fermentation efficiency Low and other problems, to achieve the effect of improving singleness, high PHA output, and high sludge output

Active Publication Date: 2016-08-17
北京中汉汇德环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the batch fermentation process has obvious defects: ① During the fermentation process, acid-base solution must be continuously added to the fermenter to control the pH, and special dosing equipment must be configured; the fermenter must be cleaned and disinfected after each cycle; It is necessary to replace the fermentation broth, etc., which consumes a lot of manpower, material resources, and power; ②The production cycle is short, because the bacteria have a certain growth law during batch fermentation, they all go through the lag phase, logarithmic growth phase, stable phase and The decline period, and each batch of fermentation must go through the stages of strain expansion fermentation, equipment washing, sterilization, etc.; ③The production efficiency is low, because the entire fermentation cycle includes the time of discharging, washing tanks, feeding, and sterilizing, it is actually effective The fermentation time is short and the fermentation efficiency is very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The specific implementation steps are as follows:

[0022] Step 1. Take the halophilic sludge from the estuary and put it into a 10L SBR reactor. Use domestic sewage to add coarse salt to prepare a culture solution with a salinity of 50g / L. The halophilic activated sludge is screened and activated. Complete aerobic aeration operation, dissolved oxygen concentration ≥ 3mg / L, aeration time 8h, precipitation 4h, then drain the supernatant, run for 40 days; wait until the sludge color changes from black brown to light yellow, and activated sludge appears When characteristics, enter the next stage of domestication and cultivation.

[0023] Step 2: Add 8L of molasses alcohol wastewater to the SBR reactor containing the halophilic activated sludge that has been domesticated in Step 1, and control the reaction conditions as room temperature, dissolved oxygen ≥3mg / L, aeration time 20h, precipitation 4h, and then Drain the supernatant; run for 25 days until the halophilic activated s...

Embodiment 2

[0028] The specific implementation steps are as follows:

[0029] Step 1. Take the halophilic sludge from the estuary and put it into a 10L SBR reactor. Use domestic sewage to add coarse salt to prepare a culture solution with a salinity of 50g / L. The halophilic activated sludge is screened and activated. Complete aerobic aeration operation, dissolved oxygen concentration ≥ 3mg / L, aeration time 8h, precipitation 4h, then drain the supernatant, run for 40 days; wait until the sludge color changes from black brown to light yellow, and activated sludge appears When characteristics, enter the next stage of domestication and cultivation.

[0030] Step 2: Add 8L of molasses alcohol wastewater to the SBR reactor containing the halophilic activated sludge that has been domesticated in Step 1, and control the reaction conditions as room temperature, dissolved oxygen ≥3mg / L, aeration time 20h, precipitation 4h, and then Drain the supernatant; run for 25 days until the halophilic activated s...

Embodiment 3

[0035] The specific implementation steps are as follows:

[0036] Step 1. Take the halophilic sludge from the estuary and put it into a 10L SBR reactor. Use domestic sewage to add coarse salt to prepare a culture solution with a salinity of 50g / L. The halophilic activated sludge is screened and activated. Complete aerobic aeration operation, dissolved oxygen concentration ≥ 3mg / L, aeration time 8h, precipitation 4h, then drain the supernatant, run for 40 days; wait until the sludge color changes from black brown to light yellow, and activated sludge appears When characteristics, enter the next stage of domestication and cultivation.

[0037] Step 2: Add 8L of molasses alcohol wastewater to the SBR reactor containing the halophilic activated sludge that has been domesticated in Step 1, and control the reaction conditions as room temperature, dissolved oxygen ≥3mg / L, aeration time 20h, precipitation 4h, and then Drain the supernatant; run for 25 days until the halophilic activated s...

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PUM

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Abstract

The invention relates to a method for producing PHA by continuous treatment of molasses alcohol wastewater via mixed halophilic bacteria and belongs to the technical field of production of PHA. The method comprises the following steps of carrying out activation culture on mixed halophilic sludge to obtain halophilic activated sludge, carrying out enrichment culture on the molasses alcohol wastewater inside an SBR reactor by virtue of mixed halophilic bacteria colony, carrying out continuous PHA production on molasses wastewater inside a continuous flow reactor and recovering PHA in the discharged residual sludge. The PHA is synthesized by virtue of the mixed bacteria colony instead of a pure bacteria colony, strict sterilization procedures is not required, the process is simplified and the cost is saved; the continuous flow process is simple, compared with the fermentation process, the repeated replacement of the fermentation broth is not required, the repeated inoculation of bacterial sludge is not required, the continuous water inlet and continuous water outlet are achieved, respective process parameters tend to a constant value and is simply implemented, the automatic control is facilitated, the method is easily operated in engineering, the yield of PHA is high, the continuous production of PHA is achieved and the resource utilization of residual sludge is also achieved.

Description

Technical field [0001] The invention belongs to the technical field of PHA production, and relates to a technical method for synthesizing polyhydroxy fatty acid ester (PHA) by treating molasses alcohol wastewater in a continuous plug flow reactor by using halophilic mixed flora. The invention is particularly suitable for the pretreatment of molasses alcohol wastewater for resource recovery, and has multiple advantages of waste utilization, resource recovery and reduction of the industrial production cost of PHA. Background technique [0002] At present, worldwide, the industrialized production of PHA generally adopts the method of pure bacterial single substrate for biological fermentation. For example, the American Metabolix company created a genetically recombined Escherichia coli K12 strain, which uses glucose as a substrate for fermentation, and can obtain 90% of the dry cell weight of PHA products in 24 hours. The German Biomer company uses Alcaligenes latus strain to ferme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62C02F3/12C02F3/34
CPCY02W10/10
Inventor 崔有为冀思远张宏宇
Owner 北京中汉汇德环境科技有限公司
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