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Method for extracting isovitexin from ficus microcarpa

A technology of isovitexin and leaflet, applied in the field of separation of natural products, can solve the problems of large solvent consumption and residue, many impurities, low extraction rate, etc., and achieves improved speed and yield, improved extraction efficiency, and high product yield. Effect

Inactive Publication Date: 2014-12-10
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for extracting isovitexin are mainly traditional maceration method, percolation method, reflux extraction method, etc. These extraction methods all have many deficiencies, such as many impurities, long extraction time, low extraction rate, large solvent consumption and residual , high cost etc.
Patent CN1289470C "Rapid Preparation Method of Several High-purity Medicinal Substances in Patrinia alba" adopts steps such as ethanol extraction, organic solvent extraction, high-efficiency countercurrent chromatography separation and purification, etc. This method takes a long time to extract, consumes a large amount of organic solvent, and is subject to Due to the constraints of instruments and equipment, the cost is high, and it is not suitable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Put 1000g of fresh or dried Ficus ficus leaves into a container, add 5 times of 80% ethanol, put them on a traditional Chinese medicine extractor, crush and extract Ficus small leaves for 5 minutes, take them out, adjust the pH value to 4.0 with acetic acid / sodium acetate, and add 1% cellulase, placed in an ultrasonic device, hydrolyzed at 250W, 40°C for 40min, filtered, the filtrate was neutralized to pH7.0 with sodium hydroxide, purified with AB-8 macroporous adsorption resin, 50% of 8BV The ethanol was eluted, the eluate was concentrated under reduced pressure, and recrystallized twice from methanol to obtain 5.3 g of isovitexin, which was detected by HPLC with a purity of 92.3%.

Embodiment 2

[0017] Put 1000g of fresh or dried Ficus ficus leaves into a container, add 8 times of 75% ethanol, put them on a traditional Chinese medicine extractor, crush and extract Ficus small leaves for 8 minutes, take them out, adjust the pH value to 5.0 with acetic acid / sodium acetate, and add 1.5% cellulase, placed in an ultrasonic device, hydrolyzed at 300W, 50°C for 50min, filtered, the filtrate was neutralized to pH7.0 with sodium hydroxide, purified with ADS-5 macroporous adsorption resin, 40% of 10BV The ethanol was eluted, the eluate was concentrated under reduced pressure, and recrystallized twice from methanol to obtain 6.2 g of isovitexin, which was detected by HPLC with a purity of 95.6%.

Embodiment 3

[0019] Put 1000g of fresh or dried Ficus ficus leaves into a container, add 10 times of 70% ethanol, put them on a traditional Chinese medicine extractor, crush and extract Ficus small leaves for 10 minutes, take them out, adjust the pH value to 5.5 with acetic acid / sodium acetate, and add 2% cellulase, placed in an ultrasonic device, hydrolyzed at 400W, 60°C for 60min, filtered, the filtrate was neutralized to pH7.0 with sodium hydroxide, purified with AB-8 macroporous adsorption resin, 45% of 9BV The ethanol was eluted, the eluate was concentrated under reduced pressure, and recrystallized twice from methanol to obtain 5.8 g of isovitexin, which was detected by HPLC with a purity of 93.8%.

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PUM

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Abstract

The invention relates to a method for extracting isovitexin from ficus microcarpa, and particularly relates to a method for extracting the isovitexin by combining an application flash extraction technology and enzymolysis-ultrasonic-assisted extraction. The method comprises the following treatment steps: placing fresh or dry ficus microcarpa into a container, adding 70%-80% ethanol, and carrying out smashing extraction on the ficus microcarpa on a traditional Chinese medicine extractor for 5-10 minutes; taking out, regulating a pH value to 4.0-5.5, and carrying out enzymolysis and ultrasonic-assisted extraction; filtering, alkalifying filter liquor for neutralization, purifying by adopting macroporous adsorption resin, and recrystallizing to obtain the high-purity isovitexin. The method disclosed by the invention is simple in process, has complementary advantages by combining two technologies, greatly shortens the extraction time and is low in cost, more favorable to environmental protection and suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for extracting isovitexin from ficus ficus leaves, in particular to a method for extracting isovitexin by combining flash extraction technology with enzymatic hydrolysis-ultrasonic assisted extraction, and belongs to the field of natural product separation. Background technique [0002] Small-leaf banyan leaves, also known as small banyan leaves and falling money, are derived from the leaves of the banyan tree of the genus Ficus in the Moraceae family. Ficus xiaoye is a commonly used Chinese herbal medicine among the people. It has a mild taste and cool nature, and has the effects of clearing away heat, detoxifying, reducing swelling, removing dampness and relieving pain. For the treatment of asthma and chronic bronchitis, it also has significant effects in the treatment of cardiovascular diseases, anti-inflammatory, antibacterial and other aspects. It mainly contains chemical components such as flavonoids, triterpeno...

Claims

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Application Information

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IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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