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A kind of extracting method and application of Tibetan edge rhubarb active ingredient

A technology of Tibetan rhubarb and active ingredients, which is applied in the field of plant active ingredient extraction, can solve the problems of long extraction time, lower ratio and absorption of active ingredients, and low extraction rate, and achieve the goal of shortening extraction time, increasing yield, and lowering blood lipids Effect

Active Publication Date: 2017-05-17
XIZANG WENSHENGJI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction time of this method is long, the extraction rate is low, the proportion of active ingredients and the absorption decrease, etc.

Method used

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  • A kind of extracting method and application of Tibetan edge rhubarb active ingredient
  • A kind of extracting method and application of Tibetan edge rhubarb active ingredient
  • A kind of extracting method and application of Tibetan edge rhubarb active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Extraction Method of Active Components of Zangbian Rhubarb

[0036] The method considers efficient utilization of resources, high extraction rate, short extraction time, and easy absorption of extracted components by the human body, etc., and adopts a biological enzymatic hydrolysis method to extract active components in Tibetan rhubarb.

[0037] combine figure 1 .

[0038] 1. Cleaning: Screen the undamaged fresh rhubarb and clean it;

[0039] 2. Beating: beat the cleaned Tibetan rhubarb to make the particle size of Tibetan rhubarb uniform, which is beneficial to enzymatic extraction;

[0040] 3. Enzyme hydrolysis:

[0041] (1) Add 65IU / 100ml 20,000U / g cellulase to the raw material, control the temperature at 40-55°C, control the pH at 3.5-6.5, and enzymolysis time 1-2h;

[0042] (2) The enzymolysis solution in the above (1) is made into a starch slurry of 16-17°Bé, the pH is adjusted to 6-7, and 0.2% CaCl is added by weight of raw materials 2 , add α-amylase to th...

Embodiment 2

[0051] Comparison experiment of extraction method

[0052] The material is divided into 6 groups on average, respectively ①, ②, ③, ④, ⑤, ⑥, each group of 10kg, ①, ②, ③ groups are extracted with the method of the present invention, and the specific implementation method is carried out according to the test steps of Example 1; ④, ⑤ and ⑥ are general extraction methods, extract with the ratio of material: water = 1:8, extract for 8 hours, filter, dry, and weigh.

[0053] The general water extraction method and the enzymatic method of the present invention extract the yield of rhubarb rhubarb effective components, and the yield obtained by using the enzymatic method to extract rhubarb is higher than that obtained by the general extraction method. From the time of extraction, the enzymatic method is also greatly shortened. The extraction time is shortened, so the rhubarb rhubarb extracted by the method of the present invention is significantly improved in time and extraction yield....

Embodiment 3

[0055] Detection experiment

[0056] The extract obtained in the present invention is subjected to the determination of anthraquinones, stilbenes and tannins.

[0057] 1. Determination of anthraquinone compounds

[0058] Anthraquinone compounds mainly detect the total anthraquinone content in the compound, and the main detection methods are as follows:

[0059] 1.1 Instruments and medicines

[0060] Ultraviolet spectrophotometer, electronic analytical balance, the obtained extract M of the present invention 1 , Extract M obtained by general water extraction 2 , 1,8-dihydroxyanthraquinone, magnesium acetate, methanol, etc., all reagents used are of analytical grade.

[0061] 1.2 Experimental method

[0062] 1.2.1 Preparation of developer

[0063] Weigh 0.4g of magnesium acetate, dissolve it in methanol to prepare a 0.6% magnesium acetate methanol solution, shake well and set aside.

[0064] 1.2.2 Preparation of standard curve

[0065] Accurately weigh 10.7 mg of 1,8-dih...

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Abstract

The invention discloses a method for extracting active ingredients of rheum australe and application thereof. The method comprises the following steps: cleaning, screening, pulping, performing enzymolysis, filtering, leaching, homogenizing, drying, screening, sterilizing and the like. Multiple active ingredients in the rheum australe are extracted by adopting an enzymic method, and the biological activity is well remained, so that the effects of inhibiting fungi, astringing, checking diarrhea, reducing blood fat and slightly causing purgation for the rheum australe are effectively and fully remained. The various active ingredients in the rheum australe are extracted by adopting the enzymic method, so that more active ingredients are extracted, damage of high-temperature extraction on functional components is reduced, and beneficial effects of the rheum australe are well exerted. According to the extraction method disclosed by the invention, the active ingredients in the rheum australe are extracted by adopting the enzymic method, the extraction time is shortened, and the yield of the rheum australe extract is improved.

Description

technical field [0001] The invention belongs to the technical field of an extraction method of active ingredients of plants and the application of the extract, and in particular relates to an extraction method of active ingredients of Rhubarb Zangbian and its application. Background technique [0002] The roots and rhizomes of Rhubarb RheumemodiWall, a plant of the Rhubarb family of the Polygonaceae Rhubarb group, are sour, bitter, and cold in nature; they are mainly distributed in central and eastern Tibet, Qinghai, Sichuan, Yunnan and other provinces, mostly wild, Tibet It is planted in some areas; Tibetan and Mongolian medicine is used to treat gastroenteritis, nephritis, external use to stop bleeding, treat sores, reduce inflammation, and heal wounds. The chemical constituents of rhubarb mainly include anthraquinones, stilbenes and tannins. Among them, the content of stilbene compounds is relatively high, especially the average content of Piceatannol-4′-O-β-D-glucopyran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/708A23L33/105A61K8/9789A61P31/10A61P1/12A61P3/06A61P35/00A61P17/16A61P29/00A61P7/02A61P9/12A61P3/10A61P37/04A61P39/06A61Q19/08A23L33/00
CPCA23V2002/00A61K8/97A61K36/708A61K2236/13A61K2236/19A61K2236/331A61K2236/51A61K2236/53A61K2800/10A61Q19/00A23V2200/30
Inventor 蒋川湘
Owner XIZANG WENSHENGJI TECH