Radiation-sterilization-resistant protein composition
A composition and protein technology, applied in the field of protein composition, can solve the problems of strong toxicity, adverse effects on the human body, and no sterilization of enzyme-containing nanofibers.
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Embodiment 1
[0069] Disperse fibrinogen freeze-dried powder (Boluhelu (registered trademark, the same below) for tissue adhesion: vial (vial) 1) in 2-propanol, and then dissolve hydroxypropyl cellulose (6-10mPa·s , manufactured by Wako Pure Chemical Industries, Ltd.) to 16 wt%, to prepare a spinning solution containing fibrinogen-containing particles / hydroxypropyl cellulose=20 (9.2 as fibrinogen) / 100 (w / w). At a temperature of 22°C and a humidity of 26% or less, spinning is performed by electrospinning to obtain a sheet-like fiber molded body. The inner diameter of the nozzle is 0.8 mm, the voltage is 11 kV, the flow rate of the spinning solution is 1.2 mL / hour, and the distance from the nozzle to the plate is 15 cm. The average fiber diameter of the obtained fiber molded product was 0.86 mu m with an average thickness of 137 mu m. The obtained sheet was sterilized by electron beam at 20 kGy. Cut the sterilized sheet into 0.5cm×0.5cm, use 62.5 mu Protein was extracted with L normal...
Embodiment 2
[0071] Freeze-dried fibrinogen powder (for tissue bonding: vial 1) was dispersed in 2-propanol, and then hydroxypropyl cellulose (6-10 mPa·s, manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved to 16 wt%, A spinning solution of fibrinogen freeze-dried powder / hydroxypropyl cellulose=40 (18 based on fibrinogen) / 100 (w / w) was prepared. At a temperature of 22°C and a humidity of 26% or less, spinning is performed by electrospinning to obtain a sheet-like fiber molded body. The inner diameter of the nozzle is 0.8 mm, the voltage is 12.5 kV, the flow rate of the spinning solution is 1.2 mL / hour, and the distance from the nozzle to the plate is 15 cm. The average fiber diameter of the obtained fiber molded product was 0.43 mu m with an average thickness of 152 mu m. The obtained sheet was sterilized by electron beam at 20 kGy. Cut the sterilized sheet into 0.5cm×0.5cm, use 62.5 mu Protein was extracted with L normal saline, and ELISA assay was carried out. As ...
Embodiment 3
[0073] Freeze-dried fibrinogen powder (for tissue bonding: vial 1) was dispersed in 2-propanol, and then hydroxypropyl cellulose (6-10 mPa·s, manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved to 16 wt%, A spinning solution of fibrinogen freeze-dried powder / hydroxypropyl cellulose=100 (46 based on fibrinogen) / 100 (w / w) was prepared. At a temperature of 22°C and a humidity of 26% or less, spinning is performed by electrospinning to obtain a sheet-like fiber molded body. The inner diameter of the nozzle is 0.8 mm, the voltage is 12.5 kV, the flow rate of the spinning solution is 1.2 mL / hour, and the distance from the nozzle to the plate is 15 cm. The average fiber diameter of the obtained fiber molded body was 0.35 mu m, with an average thickness of 191 mu m. The obtained sheet was sterilized by electron beam at 20 kGy. Cut the sterilized sheet into 0.5cm×0.5cm, use 62.5 mu Protein was extracted with L normal saline, and ELISA assay was carried out. As a...
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