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Preparation method and application of dictyophora indusiata polysaccharide-zinc chelate with antitumor activity

An anti-tumor activity, a technology of bamboo fungus polysaccharide, applied in the directions of anti-tumor drugs, organic active ingredients, medical preparations containing active ingredients, etc., to achieve the effects of simple preparation process and product safety

Active Publication Date: 2015-01-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In summary, there are no reports on the antitumor activity of polysaccharide-zinc chelates.

Method used

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  • Preparation method and application of dictyophora indusiata polysaccharide-zinc chelate with antitumor activity
  • Preparation method and application of dictyophora indusiata polysaccharide-zinc chelate with antitumor activity
  • Preparation method and application of dictyophora indusiata polysaccharide-zinc chelate with antitumor activity

Examples

Experimental program
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Embodiment 1

[0029] The preparation method of the Sevage reagent described in Example 1 and Example 2 is to mix chloroform and n-butanol in a volume ratio of 4:1, and the method of Sevage deproteinization is to mix polysaccharide and Sevage reagent in a volume ratio of 1:1, shake Centrifuge at 3000r / min for 10min for 20min, take out the polysaccharide solution in the upper layer, and repeat the above steps 5 times.

[0030] Example 1:

[0031] (1) Extraction of Dictyophora polysaccharides: crush 100 g of dried Dictyophora fruiting bodies, shake in a boiling water bath for 2 hours to extract polysaccharides, filter, concentrate, and vacuum freeze-dry;

[0032] (2) Separation of Dictyophora polysaccharide: After deproteinizing Dictyophora polysaccharide by Sevage method, add ethanol, centrifuge at 3000r / min for 20min, discard the supernatant, dissolve the precipitate in distilled water, repeat 5 times, and freeze the obtained solution dry;

[0033] (3) Purification of Dictyophora polysacch...

Embodiment 2

[0036] (1) Extraction of Dictyophora polysaccharides: crush 100 g of dried Dictyophora fruiting bodies, shake in a boiling water bath for 2 hours to extract polysaccharides, filter, concentrate, and vacuum freeze-dry;

[0037] (2) Separation of Dictyophora polysaccharide: After deproteinizing Dictyophora polysaccharide by Sevage method, add ethanol, centrifuge at 3000r / min for 20min, discard the supernatant, dissolve the precipitate in distilled water, repeat 5 times, and freeze the obtained solution dry;

[0038] (3) Purification of Dictyophora polysaccharide: dissolve the Dictyophora polysaccharide obtained in step (2) in water, purify with DEAE-52 cellulose ion exchange chromatography column, use 0.05mol / L NaCl solution as eluent, collect and wash Remove the liquid, then purify with Sephadex G-200 Sephadex chromatographic column, use 0.05mol / L NaCl solution as the eluent, collect the eluate, concentrate, and freeze-dry to obtain Dictyophora polysaccharide;

[0039] (4) Inorg...

Embodiment 3

[0043] (1) Extraction of Dictyophora polysaccharides: crush 100 g of dried Dictyophora fruiting bodies, shake in a boiling water bath for 6 hours to extract polysaccharides, filter, concentrate, and vacuum freeze-dry;

[0044] (2) Separation of Dictyophora polysaccharide: After deproteinizing Dictyophora polysaccharide by Sevage method, add ethanol, centrifuge at 6000r / min for 10min, discard the supernatant, dissolve the precipitate in distilled water, repeat 3 times, and freeze the obtained solution dry;

[0045] (3) Purification of Dictyophora polysaccharide: Dissolve the Dictyophora polysaccharide obtained in step (2) in water, purify it with DEAE-52 cellulose ion exchange chromatography column, use 2mol / L NaCl solution as eluent, collect and elute liquid, and then purified by Sephadex G-200 Sephadex chromatographic column, using 2mol / L NaCl solution as eluent, collecting the eluate, concentrating, and freeze-drying to obtain the Dictyophora polysaccharide used for chelatio...

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Abstract

The invention discloses a preparation method of a dictyophora indusiata polysaccharide-zinc chelate with antitumor activity. The method comprises the following steps of (1) extracting dictyophora indusiata polysaccharide; (2) separating the dictyophora indusiata polysaccharide; (3) purifying the dictyophora indusiata polysaccharide; (4) adding an inorganic zinc source in a dictyophora indusiata polysaccharide solution to carry out chelation reaction; (5) carrying out alcohol precipitation; and (6) carrying out freeze drying to obtain the dictyophora indusiata polysaccharide-zinc chelate. The dictyophora indusiata polysaccharide-zinc chelate researched by the invention is effectively applied to preparation of zinc supplementing health products and medicines; and proven by an MTT method, a flow cytometry method, a Hoechst33258 staining method and a DNALadder and JC-1 staining method, the chelate has remarkable antitumor bioactivity so as to be expected to become an efficient and low-toxicity drug for treating cancers particularly liver cancer.

Description

technical field [0001] The invention relates to a preparation method and application of an anti-tumor substance, in particular to a preparation method and application of a polysaccharide-zinc chelate with anti-tumor activity. Background technique [0002] Zinc is an essential trace element for the human body. It is a component or coenzyme of 200 kinds of metalloenzymes in the human body. Zinc deficiency in the body will lead to decreased basal metabolism, decreased protein utilization, decreased appetite and digestive function, and affected growth and development. In the past and now, zinc supplementation mostly uses preparations such as zinc sulfate and simple zinc gluconate, and most of them are absorbed in the form of ions in the body. On the one hand, zinc ions are easy to combine with phytic acid, oxalic acid, fatty acid and other substances in the diet, and easily form insoluble matter, resulting in a large loss; on the other hand, due to factors such as insufficient ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCA61K31/715C08B37/00
Inventor 杨继国张榕廖文镇任娇艳林泽华宁正祥
Owner SOUTH CHINA UNIV OF TECH
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