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Pretreatment method for chlorpromazine detection in animal product

An animal product, chlorpromazine technology, applied in the field of food safety, can solve the problems of cumbersome and time-consuming sample processing, lack of wide applicability, poor reproducibility, etc., and achieve wide application range, high recovery rate, and reproducibility Good results

Inactive Publication Date: 2015-01-14
CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are sensitive and accurate, but the sample processing is cumbersome and time-consuming, especially the gas chromatography-mass spectrometry (GC-MS) method, which generally has a relatively low recovery rate caused by poor sample pretreatment, and the reproducibility is not good. A sample pretreatment method with high operational accuracy, high reproducibility, and high sensitivity is promoted and applied
Chinese patent (CN103454368A) discloses a method for HPLC detection of chlorpromazine content in blood, which is limited to detection of chlorpromazine content in blood samples; Ministry of Agriculture Announcement No. 1163-8-2009 Chlorpromazine in pig liver The standard of gas chromatography-mass spectrometry for residue detection is also limited to the detection of chlorpromazine content in animal liver, which does not have wide applicability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A pretreatment method for detection of chlorpromazine in animal products, consisting of the following steps:

[0025] 1) Weigh 1.0 g of the homogenized animal product and place it in a 100 mL centrifuge tube, add 15 mL of ethyl acetate and 1.0 mL of 2 mol / L NaOH solution in sequence, homogenize for 1 min, vortex shake for 15 min, and centrifuge at 3000 r / min for 5 min;

[0026] 2) Take the supernatant and transfer it to a rotary evaporator, then use 10mL ethyl acetate for the sample, and repeat the extraction once, and then use 5-15mL ethyl acetate and 0.5mL NaOH solution with a concentration of 2.0mol / L for the lower sample , homogenize for 1 min, vortex shake for 15 min, centrifuge at 3000r / min for 5 min and repeat the extraction once, combine the supernatant, and spin evaporate to dryness;

[0027] 3) Use 2 mL of acetonitrile to ultrasonically dissolve the residue, add 4 mL of n-hexane, vortex for 1 min, centrifuge at 1000 r / min for 5 min, discard the n-hexane layer,...

Embodiment 2

[0032] A pretreatment method for detection of chlorpromazine in animal products, consisting of the following steps:

[0033] 1) Weigh 0.5g of the homogenized animal product into a 100mL centrifuge tube, add 20mL of ethyl acetate and 0.5mL of 1.5mol / L NaOH solution in sequence, homogenize for 0.5min, vortex shake for 20min, and centrifuge at 2000r / min for 8min ;

[0034] 2) Take the supernatant and transfer it to a rotary evaporating flask, use 5mL ethyl acetate for the sample, and repeat the extraction once, and then use 5-15mL ethyl acetate and 1mL NaOH solution with a concentration of 1.5mol / L for the lower sample, Homogenize for 0.5 min, vortex shake for 20 min, centrifuge at 2000r / min for 8 min to repeat the extraction once, combine the supernatant, and spin evaporate to dryness;

[0035] 3) Use 2.5mL acetonitrile to sonicate the residue, add 3mL n-hexane, vortex for 2min, centrifuge at 500r / min for 8min, discard the n-hexane layer, and set aside;

[0036] 4) Rinse the P...

Embodiment 3

[0040] A pretreatment method for detection of chlorpromazine in animal products, consisting of the following steps:

[0041] 1) Weigh 1.5g of the homogenized animal product into a 100mL centrifuge tube, add 10mL of ethyl acetate and 1.5mL of 1.5mol / L NaOH solution in sequence, homogenize for 3min, vortex shake for 10min, and centrifuge at 3500r / min for 3min;

[0042] 2) Take the supernatant and transfer it to a rotary evaporating flask, then use 15mL ethyl acetate for the sample, and repeat the extraction once, and then use 5-15mL ethyl acetate and 0.3mL NaOH solution with a concentration of 1.5mol / L for the lower sample , homogenize for 3 minutes, vortex shake for 10 minutes, centrifuge at 3500r / min for 3 minutes to repeat the extraction once, combine the supernatants, and spin evaporate to dryness;

[0043] 3) Use 1.5mL acetonitrile to ultrasonically dissolve the residue, add 5mL n-hexane, vortex for 0.5min, centrifuge at 1500r / min for 3min, discard the n-hexane layer, and s...

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Abstract

The invention belongs to the technical field of food safety, and particularly relates to a pretreatment method for chlorpromazine detection in an animal product. The pretreatment method disclosed by the invention comprises the following operations: weighing of a to-be-detected sample, extraction, cyclotron oscillation, centrifugation, solid-phase extraction and the like, wherein the used apparatus comprises a centrifugal machine, rotary evaporation and solid-phase extraction devices and the like. A sample solution prepared by using the pretreatment method can be directly detected by virtue of a gas chromatography-mass spectrometry method. Compared with an existing method, the pretreatment method has the advantages of being high in operation accuracy, high in repeatability, high in sensitivity and the like, has strong operability, is expected to be popularized and applied on a large scale in the industries such as agriculture, quality inspection departments, and entry and exit inspection and quarantine, and has significant economic benefits. According to the pretreatment method, whether chlorpromazine is contained in the animal product can be accurately detected; and the detected animal product is safe to eat and accords with the requirements of the national standard in China.

Description

technical field [0001] The invention belongs to the technical field of food safety, and in particular relates to a pretreatment method for detecting chlorpromazine in animal products. Background technique [0002] Chlorpromazine is a representative drug of phenothiazines. It is a blocker of central dopamine receptors and acts on the central nervous system. It is often used as a hypnotic and sedative. It can significantly reduce the mortality of animals during transportation. This drug is mainly metabolized in the liver, and it is easy to produce drug residues, which has a great impact on people's health. In the import and export trade, it has been listed as a prohibited drug, and it is not allowed to be detected in our country. [0003] Existing methods for detecting chlorpromazine content in animal products include high-pressure liquid chromatography (HPLC), high-pressure liquid chromatography / fluorescence (HPLC / FLD), liquid chromatography-mass spectrometry (LC-MS), gas ch...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/14
Inventor 路平
Owner CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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