2-(1-n-hexyloxy)ethylchlorin f salt, and pharmaceutical composition and application thereof

A technology of ethyl chlorin and n-hexyloxy, which is applied in the direction of drug combination, pharmaceutical formula, antineoplastic drugs, etc., can solve the problems of increasing the production cost of photosensitizers, and achieve short retention time, strong photodynamic activity, dark less toxic effect

Active Publication Date: 2015-01-21
RAINBOW PHARMATECH
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, this technology has shown advantages in in vitro studies, but the use of capsules made of nanoparticle ceramic materials will greatly increase the production cost of photosensitizers, and the above-mentioned effects of this new dosage form have not been confirmed in clinical trials

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 2-(1-n-hexyloxy)ethylchlorin f salt, and pharmaceutical composition and application thereof
  • 2-(1-n-hexyloxy)ethylchlorin f salt, and pharmaceutical composition and application thereof
  • 2-(1-n-hexyloxy)ethylchlorin f salt, and pharmaceutical composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: Preparation of 2-(1-hexyloxy)ethyl chlorin f sodium salt (HPS)

[0058] step 1.The preparation of the chlorin f of formula 2

[0059]

[0060] Dissolve 660mg of pheophorbide a methyl ester of formula 1 in pyridine (4ml), add 25% KOH-MeOH solution (50ml), and pass O at 0°C 2 reacted for 1 hour, and then introduced N 2 20 minutes, quickly boil and reflux for 40 minutes, cool slightly, add water (200ml), and use 10% H 2 SO 4 Neutralize to pH 5-6, filter with suction, and after the filter cake is dried, go through silica gel column chromatography (chloroform:methanol=20:1), separate and obtain the chlorin f of formula 2 (black powder, 70mg, yield 12%) .

[0061] Compound of formula 2: UV-vis[CH 3 OH,λ max (nm)(ε)]:662(121104),498(41964),398(436694); 1 H NMR (400MHz, DMSO-d 6 )δ:9.88(1H,s,β-H),9.85(1H,s,γ-H),9.74(1H,s,α-H),9.05(1H,s,δ-H),8.33(1H ,dd,J=18.0,11.6Hz,2a-H),6.45(1H,d,J=18.0Hz,2b-H B ),6.21(1H,d,J=12.0Hz,2b-H A ),4.63(1H,m,8-H),4.47...

Embodiment 2

[0071] Embodiment 2: Preparation of 2-(1-hexyloxy)ethyl chlorin potassium salt (HPK)

[0072] 2-(1-hexyloxy)ethyl chlorin potassium salt (HPK) was prepared in a similar manner to Example 1, except that in step 3, the isopropanol solution of potassium hydroxide was used to replace Sodium hydroxide solution in isopropanol.

[0073] HR-ESI-MS m / z718.00945 gives quasi-molecular ion peak [M+H] + , corresponding to the formula C 38 h 47 N 4 o 5 K 2 (calculated value 718.00833), with an unsaturation of 16.5.

[0074]

Embodiment 3

[0075] Example 3: Activity of Compounds of the Invention as Photosensitizers

[0076] The resuscitated mouse sarcoma S180 cells were inoculated into the peritoneal cavity of female Kunming mice. After 2-3 generations of passage, when the number of viable cells reached more than 90% (tested by trypan blue), they were collected from the peritoneal cavity of mice under sterile conditions. Extract the milky white tumor fluid, add normal saline, and dilute to a cell density of about 1×10 7 Tumor cell suspension, set aside.

[0077] Another 30 female Kunming mice were taken, and 0.2ml of tumor cell suspension was inoculated subcutaneously on the right shoulder blade of each mouse. 6 mice in each group were randomly divided into 5 groups, and 48 hours after inoculation, the mice were administered separately from the tail vein of each group.

[0078] Blank control group (normal saline);

[0079] Positive control group (HPPH0.3mg / kg);

[0080] High dose group (HPS0.32mg / kg);

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a 2-(1-n-hexyloxy)ethylchlorin f salt, and a pharmaceutical composition and an application thereof. Specifically, the invention relates to the 2-(1-n-hexyloxy)ethylchlorin f salt used in photo-dynamic therapy and / or sono-dynamic therapy. The salt is soluble in water, and has stable properties. The salt can be used as a photosensitizer and / or a sound-sensitizer. The salt is suitable to be used for treating and diagnosing relatively large malignant, precancerous lesions or benign lesions. The invention also relates to a pharmaceutical composition comprising the novel compound, and the application and a preparation method of the novel compound.

Description

technical field [0001] The present invention relates to novel compounds for use in Photodynamic therapy (PDT) and / or Sonodynamic therapy (SDT). Specifically, the present invention relates to 2-(1-n-hexyloxy)ethyl chlorin f salt, which is soluble in water, stable in properties, can be used as a photosensitizer and / or sound sensitizer, and is suitable for treating and diagnosis of larger malignant tumors as well as precancerous or benign lesions. The present invention also relates to pharmaceutical compositions comprising the novel compounds, uses and preparation methods of the novel compounds. Background technique [0002] In recent years, cancer has become one of the major diseases that seriously threaten human health. It is reported that cancer has become the first cause of death among Chinese patients, and one out of every four Chinese people who died died of cancer. The incidence of cancer is increasing at an average annual rate of 3%-5%. Due to the serious side effect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22A61K41/00A61P35/00
CPCA61K41/0033A61K41/0071C07D487/22
Inventor 方起程方薇
Owner RAINBOW PHARMATECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap