A strain of Lactobacillus acidophilus capable of reducing drug resistance and its biological preparation

A technology of Lactobacillus acidophilus and biological preparations, applied in the field of Lactobacillus acidophilus, can solve the problems such as the inability to reduce the resistance of bacteria to antibiotics, and achieve the effects of reducing the difficulty of treatment and reducing drug resistance

Active Publication Date: 2017-09-19
HESHAN SEWETE BIOLOGICAL PROD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, the research and application of compound microbial bacterial agents have been very common. These microbial bacterial agents are formed by compound culture of multiple strains of a certain genus, and some are composed of multiple strains of one or more genera. Lactic acid bacteria, yeast, bacillus, bifidobacterium, etc. are the most widely used at present. These complex microbial agents with different formulas have been widely used in animal husbandry, aquaculture, and human health care. In the field, the most widely used yogurt products made of lactic acid bacteria, as well as EM bacteria, enzyme bacteria, probiotics, etc., the application functions of these products are basically positioned to improve the immunity of animals and humans, and balance the intestinal tract. The micro-ecological flora structure of the Tao, improving the micro-ecological environment, etc., so far all biological agents have not been found to have the ability to reduce the proportion of drug-resistant bacteria, and cannot reduce the resistance of bacteria to antibiotics in animals or humans

Method used

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  • A strain of Lactobacillus acidophilus capable of reducing drug resistance and its biological preparation
  • A strain of Lactobacillus acidophilus capable of reducing drug resistance and its biological preparation

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Embodiment 1

[0028] Domestication and screening of Lactobacillus acidophilus XD13R strain: 4 strains of Bacillus (XD05B-XD08B), 3 strains of lactic acid bacteria (XD05R-XD07R), and 1 strain of yeast (XD05J) were purchased from the Culture Collection Center of Guangdong Institute of Microbiology. Guangzhou Baijia Supermarket purchased 2 strains of yeast (XD06J-XD07J), and then isolated and screened 8 strains of lactic acid bacteria (XD08-RXD15R) that were different from the above-mentioned strains in plaque shape and cell shape from nature, and collected a total of 4 strains Bacillus (XD05B-XD08B), 11 strains of lactic acid bacteria (XD05R-XD15R), and 3 strains of yeast (XD05J-XD07J), and then they were subjected to 10 generations of mutagenesis culture in pig manure environment with high proportion of drug-resistant bacteria, and finally selected A bacterial strain that can adapt to the above-mentioned pig manure water environment and can reduce the proportion of drug-resistant bacteria is ...

Embodiment 2

[0032] (1) Preparation of Lactobacillus acidophilus XD13R bacterial liquid: inoculate fresh culture of Lactobacillus acidophilus XD13R into 10~20 500ml Erlenmeyer flasks filled with MRS medium, and cultivate in an incubator at 30°C 48h, to obtain Lactobacillus acidophilus XD13R bacterial liquid;

[0033] (2) Preparation of medium: with sucrose, peptone, yeast extract, KH 2 PO 4 , tomato juice and water are the main raw material preparation medium, and their weight percentages are:

[0034] Sucrose 4%, peptone 2.5%, yeast extract 1%, KH 2 PO 4 1%, tomato juice 4%, water balance.

[0035] (3) Take the above-mentioned Lactobacillus acidophilus XD13R bacterial liquid and inoculate it into the above-mentioned culture medium sterilized by high temperature, adjust the initial pH to 5~6.5, carry out anaerobic fermentation in the fermenter for 15 days, until the final pH is 3~6.5 4. Obtain the biological preparation prepared by the Lactobacillus acidophilus XD13R.

Embodiment 3

[0037] (1) Preparation of Lactobacillus acidophilus XD13R bacterial liquid: Inoculate fresh culture of Lactobacillus acidophilus XD13R into 10~20 500ml Erlenmeyer flasks filled with MRS medium, and cultivate in an incubator at 38°C 40h, to obtain Lactobacillus acidophilus XD13R bacterial liquid;

[0038] (2) Preparation of medium: with sucrose, peptone, yeast extract, KH 2 PO 4 , tomato juice and water are the main raw material preparation medium, and their weight percentages are:

[0039] Sucrose 4%, peptone 2.5%, yeast extract 1%, KH 2 PO 4 1%, tomato juice 4%, water balance.

[0040] (3) Take the above-mentioned Lactobacillus acidophilus XD13R bacterial liquid and inoculate it into the above-mentioned culture medium that has been sterilized at high temperature, adjust the initial pH to 5~6.5, and carry out anaerobic fermentation in the fermenter for 4 days, until the final pH is 3~6.5. 4. Obtain the biological preparation prepared by the Lactobacillus acidophilus XD13...

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Abstract

A strain of Lactobacillus acidophilus capable of reducing drug resistance and the biological preparation thereof. The invention discloses a new strain of Lactobacillus acidophilus (Lactobacillus acidophilus) XD13R, its preservation number is CCTCC M 2014216, the strain has the ability to reduce the proportion of drug-resistant bacteria in animals, and can reduce the resistance of animals and humans to various antibiotics . At the same time, the invention also discloses a biological preparation and a method prepared by the Lactobacillus acidophilus XD13R. Using the biological preparation to feed livestock and poultry animals can reduce the proportion of bacteria resistant to different antibiotics in animals by 30-96%.

Description

technical field [0001] The invention relates to a strain of Lactobacillus acidophilus capable of reducing drug resistance in animals, in particular to a strain of Lactobacillus acidophilus capable of reducing the proportion of drug-resistant bacteria in animals. The invention also relates to a biological preparation and a method prepared by using the new strain of Lactobacillus acidophilus. Background technique [0002] In the past 20 years, many diseases, such as tuberculosis and pneumonia, which have almost disappeared or are generally considered not to pose a major threat to human health, with the outbreak of infectious diseases and the growth of drug-resistant strains and their global re-spread, Once cast a pale shadow on the health of all mankind. In fact, we are entering the so-called "post-antibiotic era" - the dark age of uncontrollable infectious diseases before antibiotics were discovered, and today, several species of bacteria are resistant to almost all antibiot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/23
CPCC12N1/20C12N1/205C12R2001/23
Inventor 杨雄
Owner HESHAN SEWETE BIOLOGICAL PROD
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