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Propagation method of oriental lily

A technology of oriental lily and lily, applied in the field of disinfection of oriental lily explants, can solve the problems of increasing technical difficulty, inability to remove viruses, cumbersome disinfection process, etc., and achieve the effect of wide use concentration, convenient material collection, and simple sterilization

Active Publication Date: 2015-01-28
NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these studies have successfully established the lily regeneration system, the virus cannot be removed from the scales and leaves, and the above-mentioned tedious disinfection process has increased the technical difficulty to a certain extent.

Method used

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  • Propagation method of oriental lily
  • Propagation method of oriental lily
  • Propagation method of oriental lily

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Sterilization method of explants

[0032] Take the unopened buds of commercially available lily orientalis and remove the leaves, leave a little petiole, wipe the surface of the explant twice with 75% alcohol, and then hold the petiole part on the outer flame of an alcohol lamp and burn it for 5- For 10 minutes, it should be heated evenly when burning, and the petioles and the gaps of the leaves should be burned heavily. Then peel off the leaves to expose parts such as anthers and filaments, and cut the filaments into small pieces about 1cm long for use.

Embodiment 2

[0033] Example 2 Induction of Filament Callus

[0034] The MS medium was prepared first, and the specific method was as follows. According to the formula in the attached table 1, a large number of elements were prepared into a 20-fold mother solution, and the other components were prepared into a 200-fold mother solution of trace elements, iron salts, and organic components. To prepare 1L medium, take 50mL, 5mL, 5mL, and 5mL of the above-mentioned mother liquors. After mixing, add 500mL of distilled water and 6g of agar powder, heat to fully dissolve, and finally add 30g of sucrose, add water to make the volume to 1L, adjust the pH value to about 6.5, and divide. Packed into tissue culture bottles. Under 121℃, 103.4kPa, autoclave for 20min (medium preparation and sterilization methods are the same below), cool to about 50℃, add picloram to the culture medium to a final concentration of 2mg L -1 After the medium is solidified, the filaments are inoculated in the medium lateral...

Embodiment 3

[0035] Example 3 Induction of adventitious shoots by callus

[0036] After the filaments formed callus, cut the callus into small pieces with a diameter of 0.5 cm and inoculated in sterilized MS+6-BA 1.0 mg L -1 In the medium, 6-BA was added before autoclaving; cultured for 6 weeks at 22-25°C, light intensity of 1000-2000 lux, and light time of 8-12 hours, adventitious buds can be seen on the callus. Can further develop to form small bulbs.

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Abstract

The invention discloses a propagation method of oriental lily. The propagation method comprises the following steps: taking thrums of the oriental lily as explants, sterilizing the thrums, inducing calluses, inducing adventive buds, proliferating the adventive buds, inducing the adventive buds to root, hardening and seedling tissue culture seedlings, transplanting the tissue culture seedlings and the like until the young plants are formed. The method is significant to lower the production cost of the oriental lily and improve the quality of lily. The method relates to selection of oriental lily explants and the sterilization method, selection of plant hormones, and optimization of induction conditions of the calluses, induction of the adventive buds and proliferation conditions, rooting culture conditions, hardening-seedling, transplanting and other conditions. The method has the characteristics of high simplicity and good feasibility in explant sterilization method, wide explant culture conditions, low culture difficulty, and high survival rate of tissue culture seedlings, and serves as a simple, efficient and quick propagation method for the oriental lily.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for sterilizing an explant of Lilium orientalis, a process of tissue culture and plant regeneration, that is, a method for multiplying Lilium orientalis. Background technique [0002] Lily is one of the best-selling flowers on the market. Oriental lily is loved by people because of its rich color, pleasant aroma, elegant posture, rich meaning and long shelf life of flowers. Lilies are usually propagated by vegetative propagation, such as the use of bulbs and scales, buds, stems, etc., but these methods will lead to serious degradation of the variety and poor disease resistance. The best way to solve this problem is to use plant tissue culture technology to obtain virus-free seedlings with stable traits. [0003] Establishing a sterile culture system is the first step in plant tissue culture, and the usual method is to use ethanol, H 2 O 2 , NaClO,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 胡正峰张科锋靳慧霞张会宁甘慧慧吕慧捷张东霞陈靳曦
Owner NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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