Artificial skin and preparation method thereof

A technology of artificial skin and treatment method, applied in medical science, prosthesis, etc., can solve the problems of increased acidity of surrounding tissue, insufficient strength, increased repair and regeneration of connective tissue, etc., and achieves simple preparation process, short production cycle and long storage time long effect

Active Publication Date: 2015-01-28
江苏德威兰医疗器械股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biodegradable materials such as glycolide are degradable and metabolizable, but if the molecular weight is small, the strength is not enough, and the molecular weight is large, it is difficult to dissolve in water, and it will degrade when dissolved, which will affect the mechanical strength of the material, and its degraded products will make it Increased acidity of surrounding tissue, aseptic inflammation
Collagen is an important component of artificial skin, a nutrient matrix for cell differentiation and growth, and can increase the repair and regeneration of connective tissue, but its disadvantages are inelasticity and low strength

Method used

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  • Artificial skin and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Prepare artificial skin, its step comprises:

[0021] Take the bovine tendon material, clean it, cut it into small pieces, soak it with a mixed aqueous solution of 0.05% peracetic acid and 1% ethanol for 10 minutes, and use absolute ethanol to soak the bovine tendon material once at room temperature. First soak for 3 hours; fully mash the soaked material, soak in 0.2% pepsin solution for 12 hours for soaking treatment, the mass volume ratio of material to solution is 1:20 (g / ml); add 30% concentrated brine Carry out salting out. Centrifuge at 4000g for 30min. The precipitate was dissolved in 0.1% acetic acid, placed in a dialysis bag and dialyzed against 1 mol / L HCL for 5 hours. Phosphate buffer was used to adjust the pH value of the collagen solution system to 5.0, and the final concentration of the collagen solution was 0.2%. The solution was placed in an ultrasonic oscillator, and the temperature was adjusted to 20° C. and the frequency was 20 Hz. Then use sodium ...

Embodiment 2

[0023] Prepare artificial skin, its step comprises:

[0024] Take the bovine tendon material, clean it up, and cut it into small pieces, soak it with a mixed aqueous solution of 5% peracetic acid and 15% ethanol for 60 minutes, and use sodium carbonate solution to soak the bovine tendon material at room temperature for 5 times. First soak for 3 hours; fully mash the soaked material, soak in 5% pepsin solution for 48 hours for soaking treatment, the mass volume ratio of material to solution is 1:50 (g / ml); add 50% concentrated brine Carry out salting out. Centrifuge at 5000g for 50min. The precipitate was dissolved in 0.2% acetic acid, placed in a dialysis bag and dialyzed against 2mol / L HCL for 15 hours. Phosphate buffer solution was used to adjust the pH value of the collagen solution system to 6.5, the final concentration of the collagen solution was 1%, and placed in an ultrasonic oscillator, the temperature was adjusted to 25° C., and the frequency was 60 Hz. Then use s...

Embodiment 3

[0026] Prepare artificial skin, its step comprises:

[0027] Take the cartilage, clean it, cut it into small pieces, soak it in 0.1% sodium hydroxide solution for 4 hours, use methanol to soak the cartilage once at room temperature, and soak it for 3 hours each time; mash the soaked material fully, 0.2% pepsin solution was used for immersion treatment for 12 hours, and the mass volume ratio of material and solution was 1:20 (g / ml); 30% concentrated brine was added for salting out. Centrifuge at 4000g for 30min. The precipitate was dissolved in 0.1% acetic acid, placed in a dialysis bag and dialyzed against 1 mol / L HCL for 5 hours. Phosphate buffer was used to adjust the pH value of the collagen solution system to 5.0, and the final concentration of the collagen solution was 0.2%. The solution was placed in an ultrasonic oscillator, and the temperature was adjusted to 20° C. and the frequency was 20 Hz. Then use sodium hydroxide solution to adjust the pH value to 7.0, place i...

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Abstract

The invention belongs to the technical field of biological materials in tissue engineering and particularly relates to artificial skin and a preparation method thereof. The preparation method comprises the following steps: carrying out cleaning, cutting, disinfecting treatment, degreasing treatment, tissue mashing treatment, acidic enzymolysis, salting out, dialyzing treatment, defoaming treatment, crosslinking treatment, modifying agent treatment, freeze drying and vacuum drying to tendon or leathery skin. The artificial skin provided by the invention not only can be used as a cover for a wound surface of a human body temporarily, but also can retain a leathery skin support for growing and repairing the wound surface permanently, and overcomes the defects, in a conventional artificial skin product, of high price, source limitation, long time for manufacturing, short saving time, and impairs to organism caused by secondary skin-grafting. The artificial skin prepared by the invention is wide in material source, short in production period, long in saving time and low in price, and is suitable for wide application.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biomaterials, and in particular relates to an artificial skin and a preparation method thereof. Background technique [0002] Skin is an important tissue organ that covers and protects the body surface. Defects and abnormalities of the skin are often caused by inflammation, ulcers, trauma, burns, postoperative tumors, and congenital deformities. For this kind of tissue defect, the method of autologous skin transplantation is mostly used at present, which not only causes new trauma defects in the donor area, but also is often limited by the source of skin donor. [0003] In order to repair and replace defective skin tissue, scientists from all over the world are devoted to the research of artificial skin, but only a few countries such as the United States have mastered this technology and realized industrialization. Some artificial skin products have been industrialized abroad, includi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/24A61L27/20
Inventor 刘泊志
Owner 江苏德威兰医疗器械股份有限公司
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