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High-sensitivity electrode type uric acid test paper and manufacturing method thereof

A high-sensitivity, electrode-type technology, applied to the electrochemical variables of materials, material analysis through electromagnetic means, and measurement devices, can solve problems such as difficult control consistency, inaccurate test results, and gaps in stability distances, and achieve High sensitivity, high sensitivity, and the effect of improving accuracy

Inactive Publication Date: 2015-02-04
GUILIN ZHONGHUI TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic reaction electrode method with biologically active enzymes involved in the reaction is affected by the activity of the biological enzyme, and it is difficult to control the consistency; the non-enzyme reaction electrode method, although the influence of the activity of the biological enzyme can be ignored, there is still a large gap between the stability and practicality
Because the uric acid content in human blood is very low, the uric acid content of normal people is usually lower than 0.36mol / L. Regardless of whether enzyme method or non-enzyme method is used to directly detect blood uric acid content, the sensitivity is not high, and the detection error is large. imprecise

Method used

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  • High-sensitivity electrode type uric acid test paper and manufacturing method thereof
  • High-sensitivity electrode type uric acid test paper and manufacturing method thereof
  • High-sensitivity electrode type uric acid test paper and manufacturing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] This embodiment provides a preparation example of an electrode-type uric acid test paper, thereby illustrating the electrochemical biosensor and the preparation method of the present invention.

[0051]First, the working electrode, reference electrode and short-circuit electrode were made on a 0.25mm thick PET plastic substrate by screen printing technology, and the working electrode, reference electrode and short-circuit electrode were made of conductive carbon ink purchased by Acheson Company. Spread the conductive ink on the PET plastic substrate by screen printing to spread a uniformly dispersed ink thin layer, and put it in an oven at 80°C to dry for 60 minutes.

[0052] Secondly, screen printing technology is used to print insulating varnish on the substrate of the printed electrode system as an electrical insulation isolation layer, and the insulating varnish is purchased by Acheson Company. Spread the insulating varnish on the PET plastic substrate by screen pri...

Embodiment 2

[0061] This example illustrates the linear relationship between uric acid concentration and current, and the method for constructing a calibration curve using the electrode-type uric acid test paper prepared in Example 1.

[0062] Collect fresh venous blood, adjust the hematocrit ratio to 40%, and prepare blood samples with uric acid concentrations of 180 μmol / L, 375 μmol / L, 500 μmol / L, 650 μmol / L, and 900 μmol / L. Place the electrode-type uric acid test paper prepared in Example 1 in a matching tester and apply a working voltage of 300mV across the working electrode and the reference electrode; take blood and add it to the sampling port, and the blood will automatically draw about 2 μL of sample through the siphon sample guide tank , enter the reaction zone and react with the reagents in the enzyme reaction membrane to form a current, and the current corresponding to the concentration of uric acid can be quickly detected on the electrochemical biosensor detection system. The li...

Embodiment 3

[0067] This embodiment illustrates with the electrode type uric acid test paper that embodiment 1 is made and the supporting tester that obtains after inputting the linear curve of embodiment 2 method calibration, and the electrode type uric acid test paper that is made with comparative example 1 and has input comparative example 2 The precision of the blood sample is tested by the supporting tester of the linear curve obtained after the calibration of the method.

[0068] Collect fresh venous blood, adjust the hematocrit ratio to 40%, and prepare blood samples with uric acid concentrations of 275 μmol / L, 450 μmol / L, and 750 μmol / L, respectively. Place the electrode-type uric acid test paper prepared in Example 1 in a matching tester and apply a working voltage of 300mV across the working electrode and the reference electrode; take blood and add it to the sampling port, and the blood will automatically draw about 2 μL of sample through the siphon sample guide tank , enter the ...

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Abstract

The invention aims at providing high-sensitivity electrode type uric acid test paper. Under the condition that the content of uric acid in blood is low, the test sensitivity of the uric acid can be improved, and the uric acid can be accurately, quickly and simply detected. The high-sensitivity electrode type uric acid test paper comprises an insulating substrate, wherein three strip-shaped conductive electrodes are arranged on the insulating substrate; an insulated isolation layer is arranged at the upper part of each conductive electrode; an electrode conduction part at the front end of each conductive electrode is exposed; a reaction window is formed in each insulated isolation layer; an enzyme reaction film is adhered to the interior of each reaction window and contains a biological reagent which reacts with a detected substance and comprises enzyme reacting with the detected substance and an electron transfer carrier; an adhesion baffle is arranged on each insulated isolation layer; a rectangular siphon sample guide groove is formed in the position, corresponding to each reaction window on the corresponding insulated isolation layer, on the corresponding baffle; an air guide hole is formed in one end, close to each electrode conduction part, of the corresponding siphon sample guide groove and penetrates through the narrow end of the corresponding adhesion baffle; a sampling opening is formed in one end, far away from each electrode conduction part, of the corresponding siphon sample guide groove.

Description

technical field [0001] The invention relates to a medical measuring device, in particular to a high-sensitivity electrode type uric acid test paper and a manufacturing method thereof. Background technique [0002] Uric acid is the final product of purine metabolism in the human body and has no physiological function in the human body. The main sources of purine in the human body are diet, nucleoprotein decomposition and de novo synthesis. Under normal circumstances, it is mainly excreted by the kidneys, and the rest is excreted through the intestines, skin, hair, etc. [0003] Due to the tense and irregular life of modern people, irregular meals and lack of adequate rest and proper exercise, it can be seen that many people are prone to gout due to excessive fatigue, excessive drinking or long-term high-fat and high-protein diet . Gout is largely caused by high levels of uric acid in the blood. Research reports or clinical literature point out that hyperuricemia can easil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 梁明龙李中
Owner GUILIN ZHONGHUI TECH DEV
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