A one-step preparing method of high-quality heparin sodium by combination of an enzymatic method and a membrane technology

A technology of heparin sodium and binding membrane, which is applied in the field of biotechnology to prepare high-quality heparin sodium, can solve the problems of export price drop, price difference, heparin sodium confusion, etc., and achieve the effect of saving water resources

Inactive Publication Date: 2015-02-11
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent period for the production of high-quality heparin is about to expire, and at the same time, generic drugs have been launched in the United States, and the export price will inevitably drop; Most of them are produced abroad, and the production technology of domestic high-quality goods is only in the hands of a few manufacturers such as Haipurui.
However, the crude product market of heparin sodium is very chaotic, mainly based on workshop-style production methods, and the stability of quality and source is not easy to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 500g of fresh intestinal mucosa, add 250ml of pure water, stir evenly, adjust the pH to 7.5, place it in a water bath at 95°C for denaturation, and keep it warm for 30min after the temperature rises to 90°C. After the denaturation is completed, place it in a cold water bath to quickly cool down to 50°C, adjust the pH to 10.0, place it in a water bath at 55°C and stir for 2 hours. Enzymolysis was carried out for 2 hours, and the pH was maintained at 9.0 throughout the enzymolysis process. After enzymatic hydrolysis, the feed solution was lowered to room temperature, adjusted to pH 6.5, stirred for 10 minutes, placed in a cold water bath for 3 hours, and centrifuged in a low-temperature (5°C) centrifuge at 4000 r / min for 15 minutes. Collect the supernatant obtained by centrifugation. Raise the temperature of the clear liquid to 30°C, and remove impurities through a microfiltration membrane. The operating conditions are pressure of 0.2MPa, temperature of 30°C, and fl...

Embodiment 2

[0029] Take 2 kg of fresh intestinal mucosa, add 1000 ml of pure water, stir evenly, adjust the pH to 7.5, place it in a water bath at 95°C for denaturation, and keep it warm for 30 minutes after the temperature rises to 90°C. After the denaturation is completed, place it in a cold water bath to quickly cool down to 50°C, adjust the pH to 10.0, place it in a water bath at 55°C and stir for 2 hours. Enzymolysis was carried out for 2 hours, and the pH was maintained at 9.0 throughout the enzymolysis process. After enzymatic hydrolysis, the feed solution was lowered to room temperature, adjusted to pH 6.5, stirred for 10 minutes, placed in a cold water bath for 3 hours, and centrifuged in a low-temperature (5°C) centrifuge at 4000 r / min for 15 minutes. Collect the supernatant obtained by centrifugation. Raise the temperature of the clear liquid to 30°C, and remove impurities through a microfiltration membrane. The operating conditions are pressure of 0.2MPa, temperature of 30°C,...

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PUM

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Abstract

The invention relates to a method of extracting heparin sodium from mucous membrane of small intestines of pigs. The fresh mucous membrane of small intestines of pigs is adopted as a raw material. Adding amounts of materials are based on the using amount of the mucous membrane of the small intestines. The method includes: pretreating the fresh mucous membrane of the small intestines of the pigs, decomposing protein combined with heparin by an alkali extraction method and an enzymolysis method, centrifuging enzymatic hydrolysate to obtain a clear liquid, removing polypeptide, nucleic acid and other impurities in the enzymatic hydrolysate by adoption of the membrane technology by utilization of molecular weight differences of the heparin and other impurities, subjecting the liquid treated by the membrane to alcohol precipitation treatment by utilization of the characteristic that the heparin sodium is insoluble in ethanol, and drying the obtained precipitate to obtain a heparin sodium product. The method of extracting the heparin sodium fully utilizes poultry internal organ resources to produce a product with high added value, and is simple and feasible in process and suitable for industrial production. The tilter of the obtained heparin sodium is 150 u / mg. The molecular weight of the obtained heparin sodium ranges between 200 Dal and 1000 Dal.

Description

technical field [0001] The invention relates to a method for intensively processing pig small intestinal mucosa, and mainly relates to a method for preparing high-quality heparin sodium using biotechnology by using pig small intestinal mucosa as a raw material. The invention adopts the combination of biological enzyme method and membrane separation technology to prepare high-quality fine heparin sodium in one step, and avoids the cumbersome process of first preparing the crude product and then using the crude product as a raw material to prepare the high-quality heparin sodium in the traditional process. Background technique [0002] my country is rich in pig small intestine resources, and the intestinal mucosa is the waste of casing production, and the content of heparin is very high, so my country mainly extracts heparin sodium from the pig small intestinal mucosa. [0003] Heparin is widely present in various organs and tissues of mammals, and is a kind of mucopolysacchar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 万端极吴正奇徐国念海文英李猷王雄司佳马超廖宇杰杨涛万莹
Owner 武汉普赛特膜技术循环利用有限公司
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