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An ionic liquid-loaded hollow liquid-core microencapsulated cell and its application

A technology of ionic liquid and air liquid, which is applied in the direction of fixing on or in the inorganic carrier, fixing on/in the organic carrier, etc., can solve the problem of high cost of using ionic liquid, reducing the amount of ionic liquid used, and the price of ionic liquid Expensive and other issues, to achieve the effect of improving mechanical strength, improving mass transfer performance, and closely connecting the three-layer structure

Active Publication Date: 2017-07-21
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, as a biocatalytic medium, ionic liquids are expensive, use a large amount, and are difficult to recycle, resulting in high cost of use, which has always been an urgent problem to be solved in the field of biocatalysis.
[0007] On the basis of the previous work above, if ionic liquids can be applied to cell immobilization technology, it will not only further promote the catalytic activity of cells, but also greatly reduce the use of ionic liquids, and facilitate the recycling of ionic liquids. The problems of high cost, large amount of use and difficulty in recycling of ionic liquids are easily solved
However, there have been no reports on the preparation of microencapsulated cells loaded with ionic liquids and their application in the field of biocatalysis.

Method used

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  • An ionic liquid-loaded hollow liquid-core microencapsulated cell and its application
  • An ionic liquid-loaded hollow liquid-core microencapsulated cell and its application
  • An ionic liquid-loaded hollow liquid-core microencapsulated cell and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A method for preparing ionic liquid-loaded hollow liquid core microencapsulated cells, comprising the following steps:

[0055] (1) Preparation containing 15g L -1 Calcium chloride, 30g·L -1 Acetic acid solution of chitosan, wherein the concentration of calcium chloride is 15g L -1 , the concentration of chitosan is 30g·L -1 , the concentration of acetic acid is 0.175mol L -1 .

[0056] (2) In the acetic acid solution that step (1) makes, add the [BMIm]PF that purity is greater than 99% 6 to a concentration of 90.0g·L -1 , and magnetically stirred it for 2h, and mixed evenly to obtain a mixed solution.

[0057] (3) Pipette 100mL of basic enzyme-producing medium into an Erlenmeyer flask, sterilize, inoculate Penicilliumpurpurogenum Li-3, cultivate at 30°C and 170r / min for 96h, centrifuge (20°C, 8000r / min, 10min), Discard the supernatant; take the bacteria, wash with 0.9% normal saline, centrifuge, weigh the wet weight of the bacteria, resuspend with 0.9% normal sa...

Embodiment 2

[0064] Load different concentrations of [BMIM]PF with 10g 6 Put the microencapsulated cells into the Erlenmeyer flask containing 100mL of normal saline, place the Erlenmeyer flask on a shaker at 32°C and 150r / min to shake, take samples at different intervals, count the number of complete microcapsules, and calculate Breakage rate of microcapsules. Then, continue to put the intact microcapsules into normal saline for detection, and investigate the loading of different concentrations of [BMIM]PF 6 The mechanical strength of the microencapsulated cells ( figure 2 ).

[0065] from figure 2 It can be seen that as the shaking time increases, the breakage rate of the microcapsules will increase continuously, that is, the mechanical strength will decrease. After shaking for 168h, no load [BMIM]PF 6 The breakage rate of the microcapsules is the highest.

[0066] This is because the capsule membrane of the microcapsule is composed of a three-layer structure of calcium alginate c...

Embodiment 3

[0070] Will load different concentrations of [BMIm]PF 6 The microencapsulated cells were placed in a vacuum drying oven at 65°C for 24 hours and then analyzed by scanning electron microscopy. 6 The microscopic morphology of the microencapsulated cells was characterized, and the analysis results are shown in Figure 3a , 3b , 3c, 3d, 3e.

[0071] Depend on Figure 3a Visible, unloaded [BMIm]PF 6 The surface of the microencapsulated cells is basically smooth with only few wrinkles, which shows that the components in the capsule of the microencapsulated cells have good compatibility.

[0072] Depend on Figure 3b ([BMIm]PF 6 The concentration is 45.0g·L -1 )with Figure 3c ([BMIm]PF 6 The concentration is 90.0g·L -1 ) can be seen, the load [BMIm]PF 6 The microencapsulated cells have more pores on the capsule, and the surface is basically smooth. When [BMIm]PF 6 The addition amount is 90.0g·L -1 , the pores on the surface of the microencapsulated cells were the most. ...

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Abstract

The invention discloses an ionic liquid-loaded hollow liquid core microencapsulation cell and an application thereof. The microencapsulation cell is obtained by the following steps: preparing a mixed solution containing calcium chloride, chitosan and [BMIm]PF6; mixing the mixed solution with a Penicillium purpurogenum Li-3 bacterial suspension to obtain a suspension; and dropwise adding the suspension into a sodium alga acid solution, so as to obtain the microencapsulation cell. The [BMIm]PF6 is added in the preparation process of the microencapsulation cell, and forms a lot of pores in a microcapsule and the surface of the microcapsule; the [BMIm]PF6 is gathered in these pores, the microcapsule surface and a hollow liquid core; the contact among three layers of structures of the microcapsule is relatively tight due to the addition of the [BMIm]PF6; the mechanical strength of the microcapsule is improved; the mass transfer performance of the microcapsule is also improved; and the biological catalytic activity of the microencapsulation cell is improved.

Description

technical field [0001] The invention belongs to the technical field of biological microcapsule immobilized cells, and in particular relates to a hollow liquid core microencapsulated cell loaded with ionic liquid and an application thereof. Background technique [0002] Ionic liquids (ILs) are organic molten salts that are liquid at room temperature, which consist of organic cations (such as alkylimidazolium ions, alkylpyridinium ions, quaternary ammonium salt ions, etc.) Compared with traditional water and organic solvents, ionic liquids have the following characteristics: 1) basically no vapor pressure, non-flammable and explosive, and excellent chemical and thermal stability; 2) good solubility for many organic and inorganic compounds, And it is easy to separate the compounds dissolved in it; 3) Enzymes or whole cells of microorganisms show higher catalytic activity, operational stability and stereoselectivity. [0003] In recent years, there have been some research repor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/08
Inventor 曹红郑兰兰欧阳巧凤张馨李春李蕾曾延波
Owner JIAXING UNIV
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