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Biosensor based on two-dimensional all-carbon nano material graphdiyne and application of biosensor

A biosensor, graphyne technology, applied in the field of biosensors, achieves the effects of good selectivity, high sensitivity, and fast detection speed

Active Publication Date: 2021-01-12
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there has been no report on the application of tyrosinase electrochemical biosensors based on graphyne-chitosan composites for the detection of bisphenols at home and abroad.

Method used

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  • Biosensor based on two-dimensional all-carbon nano material graphdiyne and application of biosensor
  • Biosensor based on two-dimensional all-carbon nano material graphdiyne and application of biosensor
  • Biosensor based on two-dimensional all-carbon nano material graphdiyne and application of biosensor

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preparation example Construction

[0040] The specific steps of the preparation of the tyrosinase electrochemical biosensor and the detection process of the sample are as follows:

[0041] (a) After graphdiyne is mixed with water, the dispersion system 1 is obtained through ultrasonic pretreatment;

[0042] (b) chitosan is dissolved in 1% acetic acid solution to obtain chitosan solution;

[0043] (c) mixing the graphdiyne dispersion with tyrosinase buffered saline solution, shaking at room temperature for 0.5 hours;

[0044] (d) Add the chitosan solution to the composite material solution formed by graphyne and enzyme molecules and mix evenly, take 5uL of the composite solution containing graphyne-tyrosinase-chitosan (GDY-Tyr-Chi) as drops Add it to the surface of the glassy carbon electrode that has been polished, and let it dry at room temperature to obtain the electrochemical biosensor.

[0045] The graphyne of the present invention is preferably synthesized by chemical in-situ reaction on the surface of t...

Embodiment 1

[0053] Characterization of two-dimensional all-carbon nanomaterial graphyne

[0054] The morphology and structure of graphyne were characterized by TEM, XRD and Raman. figure 1 , figure 2 and image 3 TEM, XRD and Raman spectra of graphyne are given. From figure 1 It can be seen from the TEM image that graphdiyne has a continuous and uniform microstructure of laminated layers. From figure 2 It can be observed from the XRD pattern in the figure that the graphyne sample only has an obvious diffraction peak at 22.4 °, indicating that the graphyne is amorphous. image 3 There are three groups of characteristic peaks in the Raman spectrum in , which are located at 1585 cm -1 The G peak (sp on the benzene ring 2 First-order Raman scattering of hybridized carbon atoms), 1369cm -1 D peak (defect in carbon material), 2123cm -1 peaks (sp hybridized carbon atoms on the alkyne group vibrate), indicating that the carbon in graphyne is only sp and sp 2 Two states of hybridizatio...

Embodiment 2

[0056] Preparation of tyrosinase electrochemical biosensor

[0057] Glassy carbon electrode assembly steps:

[0058] (a) Polish the surface of the glassy carbon electrode with aluminum oxide powders with particle sizes of 1 μm, 0.3 μm, and 0.05 μm in sequence, then repeatedly ultrasonically clean it in absolute ethanol and deionized water, and then clean the surface of the electrode with high-purity nitrogen gas. Blow dry and set aside. Put it into 1 mmol / L potassium ferricyanide / potassium ferrocyanide solution (molar ratio 1:1), and scan the cyclic voltammetry curve between -0.1 and +0.6 V. The redox peak potential difference of the curve is less than 70mV, indicating that the redox reaction on the surface of the glassy carbon electrode is a completely reversible reaction, and the electrode polishing is in good condition, and the next experiment can be carried out.

[0059] (b) Construction of graphyne-tyrosinase composites. After the graphyne was mixed with water, the gra...

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Abstract

The invention discloses an electrochemical biosensor based on graphdiyne as well as a preparation method and application thereof, and the preparation method comprises the following steps: immobilizinga mode enzyme on the surface of the graphdiyne, mixing with a film-forming material to generate a composite material, and immobilizing the composite material on the surface of a glassy carbon electrode to obtain the electrochemical biosensor. The advantages of the graphdiyne which is a novel two-dimensional all-carbon nano material, such as a planar structure similar to graphene and other two-dimensional materials, a large comparison area, good aqueous phase dispersibility and a high conjugate structure, are fully utilized, the graphdiyne is used as an enzyme carrier of the sensor to preparethe biosensor, the preparation process is simple, and the cost is low; the complex sample pretreatment process is not needed, the detection sensitivity to bisphenol compounds is high, the detection limit is low, and the reproducibility is good; and the biosensor is quick in response to a target compound, easy to miniaturize, and suitable for point field detection, continuous online monitoring andthe like.

Description

technical field [0001] The invention belongs to the field of biosensors, in particular to an electrochemical biosensor and application of a novel two-dimensional full-carbon nanomaterial graphyne immobilized model enzyme. Background technique [0002] Bisphenol A, namely 2,2-bis(4-hydroxyphenyl)propane, referred to as BPA, is an endocrine disruptor containing two phenolic hydroxyl groups, which can affect the brain, thyroid, ovary and reproductive organs of humans and animals. Features. Bisphenol A is an important organic chemical raw material, which is mainly used to prepare various polymer materials such as epoxy resin and polycarbonate. Epoxy resins are mainly used as protective layers and linings for cans and metal bottle caps in contact with food, while polycarbonate plastics are widely used to produce food packaging bags and plastic bottles. The daily necessities containing bisphenol A that can be seen everywhere in life affect people's physical and mental health all...

Claims

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Application Information

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IPC IPC(8): C01B32/205C01B32/15G01N27/30G01N27/327
CPCC01B32/205C01B32/15G01N27/308G01N27/327
Inventor 吴令霞卢宪波陈吉平
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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