An electrochemical biosensor based on two-dimensional titanium carbide and its application

A biosensor, electrochemical technology, applied in the field of biosensors, to achieve the effects of simple sample pretreatment, low preparation cost, and ultra-high sensitivity

Active Publication Date: 2020-07-28
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there is no MXene-Ti-based 3 C 2 -A report on tyrosinase electrochemical biosensor prepared from chitosan composite material for detection of phenolic compounds

Method used

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  • An electrochemical biosensor based on two-dimensional titanium carbide and its application
  • An electrochemical biosensor based on two-dimensional titanium carbide and its application
  • An electrochemical biosensor based on two-dimensional titanium carbide and its application

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Experimental program
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Effect test

preparation example Construction

[0040] The specific steps of the preparation of the tyrosinase electrochemical biosensor and the detection process of the sample are as follows:

[0041] (a) MXene-Ti 3 C 2 After mixing with water and ultrasonic pretreatment, MXene-Ti 3 C 2 of the dispersion.

[0042] (b) dissolving chitosan in 2% acetic acid solution to obtain chitosan solution.

[0043] (c) MXene-Ti 3 C 2 The dispersion was mixed with tyrosinase phosphate-buffered saline and shaken at room temperature for 0.5 hours.

[0044] (d) Chitosan solution was added to MXene-Ti 3 C 2 Mix well with the composite material solution formed by enzyme molecules, take 5 μL of the above-mentioned MXene-Ti 3 C 2 - A composite solution of tyrosinase-chitosan (MXene-Tyr-Chi) was added dropwise to the surface of the polished glassy carbon electrode, and left to dry at room temperature to obtain an electrochemical biosensor.

[0045] MXene-Ti of the present invention 3 C 2 Preferable chemical liquid phase etching method...

Embodiment 1 2

[0050] Embodiment 1 two-dimensional crystal material MXene-Ti 3 C 2 The preparation and characterization of

[0051] Preparation of MXene-Ti by chemical liquid phase etching 3 C 2 : First put 1g Ti 3 AlC 2 The powder was slowly added to 120mL of 40% hydrofluoric acid solution, and stirred and reacted at room temperature for 72 hours; the obtained product was centrifuged to collect solid matter, washed repeatedly with deionized water, and dried in a vacuum oven for later use. The drying temperature is 60°C, and the drying time is 12 hours.

[0052] The MXene-Ti prepared above was analyzed by TEM and XRD 3 C 2 The morphology and structure were characterized. Picture 1-1 , Figure 1-2 MXene-Ti 3 C 2 TEM and XRD patterns. from Picture 1-1 As can be seen from the TEM images in the MXene-Ti 3 C 2 It has a multi-layer sheet structure similar to graphene, and its large specific surface area can provide a large number of active sites for enzyme attachment, and provide a ...

Embodiment 2

[0053] Embodiment 2 Tyrosinase electrochemical biosensor preparation

[0054] Glassy carbon electrode assembly steps:

[0055] (a) Polish the surface of the glassy carbon electrode with aluminum oxide powders with particle sizes of 1 μm, 0.3 μm, and 0.05 μm, and then repeatedly ultrasonically clean it in absolute ethanol and deionized water, and then blow the electrode surface with high-purity nitrogen. Dry and set aside. Put in 1mmol L -1 In the potassium ferricyanide / potassium ferrocyanide solution (molar ratio 1:1), scan the cyclic voltammetry curve between -0.1V and +0.6V. The redox peak potential difference of the curve is less than 70mV, indicating that the redox reaction on the surface of the glassy carbon electrode is a completely reversible reaction, and the electrode polishing is in good condition, and the next experiment can be carried out.

[0056] (b) MXene-Ti 3 C 2 - Construction of tyrosinase composites. MXene-Ti 3 C 2 After mixing with water, 0.8mg / mL M...

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Abstract

The invention discloses an electrochemical biosensor based on MXene-Ti<3>C<2> and a preparation method and application of the electrochemical biosensor. According to the preparation method, first, a pattern enzyme is fixedly carried on the surface of MXene-Ti<3>C<2>, wherein the surface is provided with -OH functional groups; and second, MXene-Ti<3>C<2> is mixed with a film-forming material to form a composite material, the composite material is fixed to the surface of a glassy carbon electrode, and the electrochemical biosensor is prepared. According to the electrochemical biosensor, the two-dimensional nanometer material MXene-Ti<3>C<2> is fully utilized to serve as an enzyme carrier of the sensor, and the electrochemical biosensor has a specific flaky structure, a large specific surfacearea, excellent metal conductivity and extremely good aqueous phase dispersity; the preparation process is simple, the prepared sensor has high sensitivity to a phenolic compound, a low detection limit and good reproducibility; a complicated sample pretreatment process is not needed in the detection process, response to a target compound is rapid, miniaturization is easy, and the electrochemicalbiosensor is suitable for on-site detection, continuous online monitoring, etc.

Description

technical field [0001] The invention belongs to the field of biosensors, in particular to a novel two-dimensional crystal material MXene-Ti 3 C 2 An electrochemical biosensor with immobilized model enzymes, and a method for detecting phenolic compounds in water environment samples, plastic products, and food and beverages. Background technique [0002] Phenolic compounds are an important chemical raw material. In the industrial manufacturing process, if the phenolic compounds in the wastewater discharged from the factory are not treated in time, it will cause very serious harm to the natural environment. Today, phenolic wastewater is one of the most harmful and most polluting industrial wastewater in the world today, and is an important source of water pollution in the environment. Among them, phenol is the most common phenolic substance in this type of sewage. As the most important phenolic substance in the sewage discharged by chemical industry, coking, gas, pharmaceuti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/327G01N27/48
Inventor 吴令霞卢宪波陈吉平
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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