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Glycolipid separation and purification method

A technology for separating and purifying glycolipids, which is applied in the field of deep processing of soybean oil leftovers, can solve the problems of low separation efficiency, difficult to meet the requirements of further research on glycolipid structure identification and biological activity, and time-consuming, and achieves the yield of glycolipids. high effect

Active Publication Date: 2015-02-18
广州海莎生物科技有限公司
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Problems solved by technology

High-performance thin-layer chromatography usually requires a large number of repeated extractions to separate a single component of glycolipids, and the amount of extraction is small, which is difficult to meet further research on glycolipid structure identification and biological activity.
Column Chromatography In recent years, column chromatography is a separation method widely used in the separation of glycolipids. Although column chromatography can process a large number of samples, it has serious irreversible adsorption, low separation efficiency, and long time-consuming And the disadvantages of large solvent consumption

Method used

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  • Glycolipid separation and purification method

Examples

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Embodiment 1

[0030] Embodiment 1: Separation and purification of glycolipids from crude phospholipids

[0031] Use a measuring cylinder to take 500ml (567g) of crude phospholipids (soybean oil leftovers) into a 2000ml large beaker, add 750ml of n-hexane, and keep stirring until the crude phospholipids are completely dissolved, then transfer them to a 2000ml separating funnel. Prepare 750ml of 50% (w / w) ethanol aqueous solution, pour 250ml of ethanol aqueous solution into the n-hexane phospholipid system for the first time, shake up and down for 5 minutes, then let stand to separate layers, take the upper ethanol aqueous solution; pour 250ml of ethanol for the second time Put the aqueous solution into the n-hexane phospholipid system, shake it up and down for 5 minutes, then let it stand for stratification, and take the upper layer of ethanol water solution; for the third time, pour 250ml of ethanol water solution into the n-hexane phospholipid system, shake it up and down for 5 minutes, th...

Embodiment 2

[0042] Embodiment 2: Separation and purification of glycolipids from crude phospholipids

[0043] Use a measuring cylinder to take 500ml (550g) of crude phospholipids into a 2000ml large beaker, add 750ml of n-hexane, and keep stirring until the crude phospholipids are completely dissolved, then transfer to a 2000ml separatory funnel. Prepare 750ml of 60% (w / w) ethanol aqueous solution, pour 250ml of ethanol aqueous solution into the n-hexane phospholipid system for the first time, shake up and down for 5min, then let stand to separate layers, take the upper layer of ethanol aqueous solution; pour 250ml of ethanol for the second time Put the aqueous solution into the n-hexane phospholipid system, shake it up and down for 5 minutes, then let it stand for stratification, and take the upper layer of ethanol water solution; for the third time, pour 250ml of ethanol water solution into the n-hexane phospholipid system, shake it up and down for 5 minutes, then let it stand for stra...

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Abstract

The invention discloses a glycolipid separation and purification method. The method comprises steps as follows: dissolving coarse phospholipid with n-hexane, then performing extraction with an ethanol aqueous solution, finally, collecting the ethanol aqueous solution, and removing the ethanol aqueous solution with a vacuum concentration method to obtain a glycolipid product. The method has the advantages that glycolipid is separated from the coarse phospholipid and purified by the aid of the difference of the solubility of the phospholipid and the glycolipid in the ethanol aqueous solution, and the obtained glycolipid is subjected to vacuum drying to form the glycolipid product with the glycolipid content higher than 80%. On one hand, the method is simple and efficient, and the glycolipid yield is high; on the other hand, the problem that the Maillard reaction affects the appearance of the phospholipid product during phospholipid processing is solved.

Description

technical field [0001] The invention relates to the field of deep processing of soybean oil leftovers, and relates to a method for separating and purifying glycolipids from soybean oil leftovers. Background technique [0002] Glycolipids are sugar-containing lipids, which are compounds formed by linking one or more monosaccharide residues with lipid moieties such as monoacyl or diacylglycerol, sphingosine, and ceramide. Glycolipids widely exist in various organisms. Glycolipids in nature can be divided into two categories according to the types of alcohol groups in their components: glyceroglycolipids and glycosphingolipids. Glycosylated glycerolipids are called glyceroglycolipids. [0003] Sugars and their complexes are a large class of substances that exist widely in nature, and are important material and energy foundations in the process of biological life. At present, people pay more attention to it as the carrier of biological information. As a large class of biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08
Inventor 李小林庞水秀
Owner 广州海莎生物科技有限公司
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