Core antigen fragment for detecting hepatitis C virus and application of core antigen fragment

Abstract

The invention provides a core antigen fragment for detecting hepatitis C virus and an application of the core antigen fragment. The antigen level of different HCV genotype serum samples is detected by using an Abbott Architect HCV quantitative core antigen kit, the sensitivity of different genotypes is compared and detected, a sample of specific genotype which influences the detection sensitivity is analyzed, a polymorphic site of the core antigen which influences the detection sensitivity of the kit is found, and the amino acid sequence of the core antigen is as shown in SEQ ID NO.1-2. The amino acid fragment of the polymorphic site of the core antigen can serve as a molecular marker of HCV core antigen detection and is used for detecting the genotype of hepatitis C virus 3b. Moreover, the existence of the molecular marker can indicate that the sensitivity of the conventional quantitative antigen detection kit is reduced when the kit is used for detecting a sample of the genotype of hepatitis C virus 3b, normalization and standardization of the core antigen detection can be facilitated, and the core antigen fragment can be used for preparing a kit for detecting the HCV 3b genotype.

Description

Technical field [0001] The present invention relates to the field of virus detection, in particular to core antigen fragments used for detecting hepatitis C virus and applications thereof. Background technique [0002] Chronic hepatitis C (CHC) caused by persistent hepatitis C virus (HCV) infection has always been a global public health problem. The onset of CHC is hidden and easily ignored by patients. It can lead to liver cirrhosis, even hepatocellular carcinoma and liver failure, posing a serious threat to human health. Therefore, the screening and early treatment of HCV infection are particularly important. The common hepatitis C genotypes in my country mainly include 1b, 2a, 3a, 3b and 6a genotypes. [0003] Anti-HCV test is the most widely used HCV infection screening test. It is easy to operate, short in time and low in cost, but its disadvantage is that the window period is long and it cannot be distinguished whether it is an active infection or the virus has been cleared...

AI Technical Summary

Problems solved by technology

[0003] Anti-HCV detection is the most widely used screening test for HCV infection. It is easy to operate, short in time, and low in cost. However, its disadvantage is that it has a long window period and cannot distinguish whether it is an active infection or the virus has been cleared. It is not suitable for immunodeficiency population; HCV RNA is the direct evidence of viral infection, and quantitative detection of HCV RNA is also a key indicator for monitoring the efficacy of CHC patients during antiviral treatment. It is easy to produce cross-contamination and aerosol pollution when operating in a small room space, so it is not suitable for small laboratories

However, in clinical testing practice, some samples of specific genotypes appeared. Although the concentration of HCV RNA was high, indicating that the virus was in the active replication phase, the result of quantification of HCV core antigen using this kit was "negative", which indicated that The current HCV detection kit still has the problem of false negatives for some samples, that is, its detection sensitivity for some positive HCV samples is significantly reduced, but no report has pointed out the reasons for the reduction in detection sensitivity of these samples

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