A gold nanoflower/quantum dot composite probe for immunofluorescence labeling and photothermal therapy of living cells

A gold nanoflower, composite probe technology, applied in the fields of nanotechnology, fluorescence/phosphorescence, nanotechnology, etc. for materials and surface science, can solve the problem of difficult to control composite probes, difficult to control consistency, and quantum dot fluorescence weakening. and other problems, to achieve the effect of high photothermal conversion efficiency, enhanced fluorescence intensity, and avoidance of biological toxicity

Inactive Publication Date: 2018-04-06
THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is easy to cause the fluorescence of quantum dots to weaken
The patent number is 201310117034.4, and the patent title is "a gold nanostar@quantum dot composite cell probe with dual functions of photothermal and fluorescence enhancement and its preparation method and application". Although the disclosed composite probe has photothermal conversion However, due to the following three aspects, some improvements are needed to make it more suitable for photothermal-fluorescent composite labeling in vivo: (1) Due to the small number of gold nanostar spikes , The length of each spike is not easy to control the consistency, resulting in a large width of its absorption spectrum, which overlaps with the absorption spectrum of near-infrared quantum dots, so that some photothermal effects will also be generated when quantum dots are excited to emit light (that is, fluorescent tracers). It has a certain impact on the brightness of near-infrared luminescence (but it has an enhancement effect on light with a wavelength shorter than orange, so it is suitable for marking superficial tumors); (2) The photothermal threshold of gold nanostars is 1.8W / cm 2 There is still room for further reduction; (3) Due to the longer spikes of gold nanostars, the 2 1. When preparing composite probes, it is not easy to control the diameter of composite probes to be less than 100nm, so it has a certain degree of negative impact on the labeling of living cells

Method used

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  • A gold nanoflower/quantum dot composite probe for immunofluorescence labeling and photothermal therapy of living cells
  • A gold nanoflower/quantum dot composite probe for immunofluorescence labeling and photothermal therapy of living cells
  • A gold nanoflower/quantum dot composite probe for immunofluorescence labeling and photothermal therapy of living cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Add 0.6mL of 10mM NaBH in ice bath 4 Add to 10mL containing 0.85mM HAuCl 4 and 0.1 M CTAB in water, the solution was aged at 29 °C for at least 3 hours. Spawns small golden balls.

[0053] (2) 6 mL of 0.5 mM HAuCl 4 The solution, 6mL of 0.2M CTAC and 4.5mL of 0.1M AA were mixed and 0.3mL of the seeds prepared in (1) were added. Centrifuge and dissolve in 1mL water. Get larger golden balls.

[0054] (3) Mix 5.8mL DMF (including 0.43mM PVP (MW=55000)) and 40uL HAuCl 4 (24.28mM) into the vial, then add 200uL of the seed crystal in step (2), and stir at 80 degrees Celsius for 1 hour to obtain the seed crystal for growing gold nanoflowers. The shape and size of the seed crystal are as follows Figure 8 (a) shown.

[0055] (4) 15mL 9M PVP in DMF solution, add 150uL HAuCl 4 , 2400uL of the seed crystal prepared in step (3) was added and stirred for a few hours until the reaction was complete. The generated gold nanoflowers were centrifuged and dissolved in alcohol ...

Embodiment 2

[0060] (1) Add 0.6mL of 10mM NaBH in ice bath 4 Add to 10mL containing 1.5mM HAuCl 4 and 0.1 M CTAB in water, the solution was aged at 29 °C for at least 3 hours. Spawns small golden balls.

[0061] (2) 6 mL of 0.5 mM HAuCl 4 The solution, 6mL of 0.2M CTAC and 4.5mL of 0.1M AA were mixed and 0.3mL of the seeds prepared in (1) were added. Centrifuge and dissolve in 1mL water. Get larger golden balls.

[0062] (3) Mix 5.8mL DMF (including 0.43mM PVP (MW=55000)) and 40uL HAuCl 4 (24.28mM) was added to the vial, followed by 200uL of the seeds from (2) and stirred at 80°C for 1 hour. A seed crystal for growing gold nanoflowers is obtained. The shape and size of the seed crystal are as Figure 8 (b) shown.

[0063] (4) 15mL 9M PVP in DMF solution, add 180uL HAuCl 4 , 2400uL (3) The prepared seed crystals were added and stirred for a few hours until the reaction was complete. The generated gold nanoflowers were centrifuged and dissolved in alcohol solution. The excitation ...

Embodiment 3

[0067] (1) Add 0.6mL of 10mM NaBH in ice bath 4 Add to 10mL containing 3mM HAuCl 4 and 0.1 M CTAB in water, the solution was aged at 29 °C for at least 3 hours. Spawns small golden balls.

[0068] (2) 6 mL of 0.5 mM HAuCl 4 The solution, 6mL of 0.2M CTAC and 4.5mL of 0.1M AA were mixed and 0.3mL of the seeds prepared in (1) were added. Centrifuge and dissolve in 1mL water. Get larger golden balls.

[0069] (3) Mix 5.8mL DMF (including 0.43mM PVP (MW=55000)) and 40uL HAuCl 4 (24.28mM) was added to the vial, followed by 200uL of the seeds from (2) and stirred at 80°C for 1 hour. A seed crystal for growing gold nanoflowers is obtained. The shape and size of the seed crystal are as Figure 8 (c) shown.

[0070] (4) 15mL 9M PVP in DMF solution, add 180uL HAuCl 4 , 1920uL seed crystals were added and stirred for a few hours until the reaction was complete. The generated gold nanoflowers were centrifuged and dissolved in alcohol solution. The excitation wavelength that pro...

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Abstract

The present invention provides a gold nanoflower structure and a preparation method thereof. The gold nanoflower structure uses gold octahedrons, gold spheres or gold tetrahedrons as seed crystals in an environment of high concentration polyvinylpyrrolidone , reducing chloroauric acid with a weak reducing agent to obtain gold nanoflower particles uniformly distributed with round-headed columns. In addition, the present invention also provides a gold nanoflower / quantum dot composite probe for immunofluorescent labeling of living cells and photothermal therapy, as well as its preparation method and application. Compared with traditional probes, this probe is a probe that integrates photothermal therapy and fluorescent labeling, which can effectively kill cancer cells in a targeted manner. Two light sources are used to achieve huge photothermal conversion efficiency and the fluorescence of quantum dots respectively. The greater enhancement of the intensity skillfully avoids the mutual interference of the two effects. The coating of silicon dioxide effectively avoids the biotoxicity of gold nanoflowers and quantum dots, and makes the surface of the composite probe easy to be functionalized with very good biocompatibility.

Description

technical field [0001] The invention relates to the technical field of composite nanoprobes, in particular to a gold nanoflower / quantum dot composite probe for immunofluorescence labeling and photothermal treatment of living cells. Background technique [0002] Quantum dots are nanomaterials artificially synthesized by a small number of atoms, also known as "artificial atoms". After being excited, they can emit fluorescence, change the structure of quantum dots, and adjust their excitation and emission wavelengths at will. It is used to label and locate the target cells after the specific antibody is activated, and maintain its physicochemical and luminescence stability in the cells for a long time. Replacing traditional dyes with quantum dots as fluorescent markers can overcome the disadvantages of large dye molecules (thus needing to make holes in cells and cannot be used for in vivo labeling), poor photochemical stability and spectral characteristics, and can be used for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F1/00B22F1/02B22F9/24C09K11/02C12Q1/02G01N21/64A61K41/00A61K9/14A61P35/00B82Y30/00B82Y40/00B22F1/054B22F1/16
CPCB22F9/24G01N21/6428A61K41/0052A61K47/6929A61P35/00B22F1/0553B22F1/054B22F1/16B22F1/056
Inventor 蒋童童朱立新许小亮
Owner THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
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