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Method for producing biologic cellulose from microorganism aerosol

A technology of biological cellulose and aerosol, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as waste, low yield, and reduced fermentation efficiency, so as to expand the range of shapes and sizes and reduce production Cost, the effect of prolonging the fermentation time

Active Publication Date: 2015-03-18
海南椰国热带水果食品加工有限公司
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AI Technical Summary

Problems solved by technology

However, with the formation of biocellulose film, it will completely cover the entire liquid surface, isolate the air needed for fermentation from microorganisms, greatly reduce the efficiency of fermentation, and even stop fermentation to a certain extent, greatly affecting the biocellulose. production speed and output
Normally, after 5-7 days of fermentation in each tray, the thickness of the biocellulose film can only reach 1-2cm, which not only lowers the yield, but also greatly limits the shape and size of the biocellulose product; The manpower and material resources required for the harvest of the film
In addition, static shallow dish culture cannot make full use of the medium, which is prone to waste and increases production costs

Method used

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  • Method for producing biologic cellulose from microorganism aerosol
  • Method for producing biologic cellulose from microorganism aerosol

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of mist aerosol

[0023] Use coconut water as the liquid medium, inoculate it with 8% by volume of Gluconacetobacter xylinum seed solution that has been cultivated to the logarithmic growth phase after sterilization, and then spray it together with sterile compressed air through a gas-liquid two-phase nozzle The mist aerosol is formed in the aerosol generation chamber, and the flow rate of sterile compressed air is 8 m 3 / h, the flow rate of the liquid medium is 200 ml / min. The aerosol preparation equipment can use a commercially available atomized aerosol generator, and its working principle is: most atomized aerosol generators have a nozzle, and compressed air is blown into the liquid to be atomized through an annular gap, and the air injection port The shear force between the liquid and the liquid fully disperses the liquid droplets in this annular gap to form fine aerosol particles. The preparation of the mist aerosol of the present i...

Embodiment 2

[0024] Embodiment 2: the preparation of mist aerosol

[0025] Take 10g of glucose, 10g of sucrose, 10g of yeast extract, 5g of peptone, and 2g of potassium dihydrogen phosphate, and dilute it to 1000ml with distilled water to make a liquid medium. After sterilization, inoculate the seeds of Gluconacetobacter xylinum that have been cultivated to the logarithmic growth phase Liquid 10% by volume; then spray it and sterile compressed air into the aerosol generating chamber through the gas-liquid two-phase nozzle to form a mist aerosol, the flow rate of sterile compressed air is 6 m 3 / h, the flow rate of the liquid medium is 100 ml / min. Use the same equipment as above.

[0026]

Embodiment 3

[0027] Embodiment 3: the preparation of mist aerosol

[0028] Get 5g of glucose, 15g of fructose, 8g of ammonium sulfate, 10g of peptone, and 1g of magnesium chloride, and dilute to 1000ml with distilled water to make a liquid medium, and inoculate 12% by volume of Acetobacter xylinum seed solution that has been cultivated to the logarithmic growth phase after sterilization; Then spray it and sterile compressed air into the aerosol generating chamber through the gas-liquid two-phase nozzle to form a mist aerosol. The flow rate of the sterile compressed air is 2 m 3 / h, the flow rate of the liquid medium is 50 ml / min.

[0029]

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Abstract

The invention relates to a method for producing biologic cellulose from microorganism aerosol. The method comprises the following steps: firstly adding a small amount of liquid culture medium in a fermentation tank, and carrying out sterilization; then preparing aseptic compressed air and the sterilized liquid culture medium which is inoculated with biologic cellulose producing bacteria into smog-like aerosol; pumping the smog-like aerosol into the fermentation tank, fermenting to produce a biologic cellulose film, and after fermenting for a period of time, complementally pumping the smog-like aerosol in each period of time. By virtue of the method, the fermenting time can be prolonged, the yield of the biologic cellulose can be increased, the fermenting efficiency can be increased, the use ratio of the culture medium can be improved, the production cost can be lowered, and the shape and size range of a biologic cellulose product can be increased and expanded.

Description

technical field [0001] The invention relates to a method for producing biological cellulose, in particular to a method for producing biological cellulose with microbial aerosol. Background technique [0002] Biocellulose is a general term for cellulose synthesized by certain microorganisms in the genera Acetobacter, Agrobacterium, Rhizobium and Sarcina, also known as bacteria Cellulose; it can form a unique ultra-fine network structure through the biosynthesis of microorganisms. With a high degree of crystallinity and polymerization, biocellulose fibers are 40-60 nanometer thick fiber bundles composed of microfibrils with a diameter of 3-4 nanometers. It has high biocompatibility, adaptability and biodegradability. At present, it has been widely used in many fields such as food, cosmetics, medicine, and papermaking. Biocellulose can be produced by static and dynamic culture. The biocellulose produced by dynamic culture has low crystallinity and often has uneven texture, s...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/02
Inventor 钟春燕钟宇光
Owner 海南椰国热带水果食品加工有限公司
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