Method for decolorizing heparin sodium and lowering dermatan sulfate content

A technology of dermatan sulfate and heparin sodium, which is applied in the fields of purification of heparin sodium, decolorization of heparin sodium and reduction of dermatan sulfate content, can solve problems affecting product quality and yield, improve product quality, reduce dosage, The effect of reducing damage

Active Publication Date: 2015-03-25
NANJING KING FRIEND BIOCHEM PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of method that can reduce the depigmentation and the content of dermatan sulfate of the heparin sodium that can reduce the destruction of heparin sodium biological activity, quality is stable, yield improves, to solve the following refining process of heparin sodium in the prior art Technical issues affecting product quality and yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The method for the depigmentation of heparin sodium and the reduction of dermatan sulfate content of the present embodiment is carried out according to the following steps:

[0014] (1) Dissolve 100g of crude heparin sodium in 1000ml of sodium acetate solution with a weight concentration of 5%, observe the dissolution phenomenon, and ensure that the crude heparin sodium is completely dissolved.

[0015] (2) Use the acetic acid solution to adjust the pH of the solution obtained in step (1), add the acetic acid solution while stirring until the pH of the obtained solution is 3.5, and stop the acid adjustment.

[0016] Using acetic acid to adjust the pH forms a buffer system in the solution, which can ensure the stability of the pH in the subsequent steps, and the solution always maintains an acidic environment system, which is beneficial to the separation of heparin sodium.

[0017] (3) Heat the solution obtained in step (2) to 60°C, then add ethanol 0.7 times the weight ...

Embodiment 2

[0025] (1) Dissolve 100g of crude heparin sodium in 1000ml of sodium acetate solution with a weight concentration of 10%, observe the dissolution phenomenon, and ensure that the crude heparin sodium is completely dissolved.

[0026] (2) Use the acetic acid solution to adjust the pH of the solution obtained in step (1), add the acetic acid solution while stirring until the pH=5.0, and stop the acid adjustment.

[0027] Using acetic acid to adjust the pH forms a buffer system in the solution, which can ensure the stability of the pH in the subsequent steps, and the solution always maintains an acidic environment system, which is beneficial to the separation of heparin sodium.

[0028] (3) Heat the solution obtained in step (2) to 60°C, then add ethanol 1.0 times the weight of the solution, stir while adding ethanol, maintain at 60°C for 1 hour, and turn off the stirring at the same time.

[0029] (4) Strictly control the temperature of the solution mixture obtained in step (3), ...

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PUM

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Abstract

The invention discloses a method for decolorizing heparin sodium and lowering dermatan sulfate content, belonging to the technical field of heparin sodium purification. The method comprises the following steps: (1) dissolving heparin sodium in a sodium acetate solution; (2) regulating the pH value of the solution obtained in the step (1) to an acidic state with acetic acid (3) heating the solution obtained in the step (2) to 60 DEG C, adding ethanol accounting for 0.7-1.0 time by weight of the solution while stirring, keeping the solution at 60 DEG C for 1 hour, and stopping stirring; (4) cooling the solution obtained in the step (3) to room temperature in a gradient cooling mode; and (5) after finishing the gradient cooling in the step (4), removing the supernate containing waste ethanol, and dehydrating and drying the subnatant precipitate solid to obtain the purified heparin sodium. The method for treating heparin sodium can effectively remove the color and lower the dermatan sulfate content in the heparin sodium.

Description

technical field [0001] The invention relates to a method for decolorizing heparin sodium and reducing the content of dermatan sulfate, which can effectively remove the color of heparin sodium and reduce the content of dermatan sulfate in heparin sodium, and belongs to the technical field of purification methods for heparin sodium. Background technique [0002] Heparin sodium is currently the most widely used anticoagulant clinically, and it appears in the form of complexes with proteins in vivo. This complex has no anticoagulant activity, and it can only exhibit anticoagulant activity if it is isolated alone. Heparin sodium is widely distributed in the biological world, and exists in higher mammals, such as lung tissue and intestinal tissue of pigs, cattle, and sheep. Common separation methods for heparin sodium include enzymatic hydrolysis or salt hydrolysis, alcohol precipitation, oxidation, etc. Due to the source and extraction method of heparin, heparin must contain a l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 唐咏群黄锡伟王春华高原董晗
Owner NANJING KING FRIEND BIOCHEM PHARMA CO LTD
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