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A Chitinase-Producing Strain and Its Application of Using Crab Shell Fermentation to Produce Chitinase

A technology of chitinase and bacterial strains, applied in the direction of enzymes, enzymes, bacteria, etc., can solve the problems of insufficient activity to meet the needs of industrialization, high production costs of enzyme preparations, and restrictions on the large-scale use of chitinase, achieving significant economic benefits and Environmental benefits, low production costs, and efficient hydrolysis effects

Active Publication Date: 2017-06-06
广州增城潮徽生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because chitinase has high economic development value and broad application prospects, there are many reports about chitinase-producing microorganisms, but there are still enzyme preparations with high production costs and insufficient activity to meet the needs of industrialization, which limits the development of chitinase. The Large-scale Application of Sulfase in Industry

Method used

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  • A Chitinase-Producing Strain and Its Application of Using Crab Shell Fermentation to Produce Chitinase
  • A Chitinase-Producing Strain and Its Application of Using Crab Shell Fermentation to Produce Chitinase
  • A Chitinase-Producing Strain and Its Application of Using Crab Shell Fermentation to Produce Chitinase

Examples

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Effect test

Embodiment 1

[0034] A method for producing chitinase by fermenting crab shells, comprising the steps of:

[0035] (1) Activation of Paenibacillus pasadenensis CS0611 The slant culture was placed in a constant temperature incubator at 37° C. for 30 minutes to activate the strain.

[0036] (2) Seed liquid culture

[0037] Take an appropriate amount of slant culture and inoculate it into the seed medium, the seed medium adopts LB medium, and the composition is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH7.0. The culture conditions were 37° C., the rotation speed of the shaker was 180 rpm, and the culture time was 20 h.

[0038] (3) Liquid fermentation culture

[0039] Transfer the seed liquid to the fermentation medium with an inoculum size of 4%. The fermentation medium is composed of: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.08g / L, potassium dihydrogen phosphate 0.02g / L, magnesium sulfate 0.04 g / L, sodium chloride 0.1g / L. The culture conditions were 30...

Embodiment 2

[0044] The difference between this embodiment and embodiment 1 is:

[0045] liquid fermentation culture

[0046] The enzyme production medium consists of: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.07g / L, potassium dihydrogen phosphate 0.03g / L, magnesium sulfate 0.05g / L, sodium chloride 0.1g / L. The inoculum size was 6%, the culture condition was 30° C., the rotation speed of the shaker was 200 rpm, and the culture time was 48 hours.

[0047] After the fermentation under the above conditions, the fermentation broth was centrifuged to obtain the supernatant, which was the crude chitinase enzyme liquid, and the enzyme activity could reach 205.4mU / mL.

Embodiment 3

[0049] The difference between this embodiment and embodiment 1 is:

[0050] liquid fermentation culture

[0051] The composition of the enzyme production medium is: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.08g / L, potassium dihydrogen phosphate 0.02g / L, magnesium sulfate 0.04g / L, sodium chloride 0.1g / L. The inoculum size was 5%, the culture conditions were 30° C., the rotation speed of the shaker was 180 rpm, and the culture time was 72 hours.

[0052] After the fermentation under the above conditions, the fermentation liquid was centrifuged to obtain the supernatant which was the crude chitinase enzyme liquid, and the enzyme activity could reach 200.2mU / mL.

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Abstract

The invention provides a chitinase-producing strain and its application to produce chitinase by fermenting crab shells. The strain is Paenibacillus pasadenensis CS0611, which has been preserved in the China Center for Type Culture Collection with a preservation number of CCTCC M2014458, date of deposit is October 8, 2014. Using this bacterium as the starting strain, using crab shells as carbon and nitrogen sources, the production of chitinase by fermentation not only has the advantages of simple fermentation technology and low production cost, the enzyme activity of the fermentation supernatant can reach 211.3mU / mL, and can Efficiently hydrolyze shrimp and crab shell waste to reduce its pollution to the environment.

Description

technical field [0001] The invention provides a strain producing chitinase and a method for producing chitinase by fermenting crab shells, belonging to the field of biotechnology. Background technique [0002] Chitinase is a kind of hydrolase that can efficiently degrade the β-1,4 glycosidic bond in chitin. It mainly exists in animals, plants, fungi, bacteria, and actinomycetes. It is used in biological control, health care and chitin resources. It has broad application prospects in the field of chemical utilization. Chitin oligosaccharides are oligosaccharides obtained by enzymatic hydrolysis of chitin. Its applications in medicine, food health care and plant defense have been discovered, and it has become a hot spot in chitin research. Studies have found that chitosan with a degree of polymerization of 2-8 plays an important role in lowering blood lipids and blood sugar, activating the body's immune system, anti-tumor and anti-infection. At the same time, it can also ext...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/42C12R1/01
CPCC12N9/2442C12Y302/01014C12N1/205C12R2001/01
Inventor 娄文勇徐培宗敏华程建华
Owner 广州增城潮徽生物技术有限公司