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VHG ethanol fermentation technology by using surfactant and water as media

A surfactant, ethanol fermentation technology, applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of increased energy consumption, prolonged fermentation time, incomplete fermentation, etc., to reduce glucose concentration, improve fermentation efficiency, reduce The effect of production costs

Inactive Publication Date: 2015-03-25
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at this stage, there are still some problems to be solved in VHG technology. On the one hand, due to factors such as product inhibition and high osmotic pressure, the fermentation time is prolonged, the fermentation is incomplete, and the cell growth rate and survival rate are reduced. The high viscosity of the medium material causes difficulty in transportation and increases energy consumption. There is no literature report on the influence of the fermentation medium on the yield and concentration of ethanol at the end point.

Method used

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  • VHG ethanol fermentation technology by using surfactant and water as media
  • VHG ethanol fermentation technology by using surfactant and water as media
  • VHG ethanol fermentation technology by using surfactant and water as media

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-9

[0039] First, weigh 0.4g of Saccharomyces cerevisiae and place it in a 100mL Erlenmeyer flask, rehydrate and activate it with 4mL of ultrapure water at 38°C for 20min, then it can be used as distiller's mother. Then add 6.0g of glucose, PEG-200 (according to the dosage in Table 1) (1-8g), pre-configured H 2 SO 4 The solution (consumption according to Table 1) was sealed with a plastic wrap, and placed in a shaker for 48 hours at 38° C. with shaking at a speed of 160 r / min. It can be seen from the data in Table 1 that when PEG-200 is not added to the fermentation system (Example 1), the conversion rate of glucose is 96%, the yield of ethanol is 81%, and the concentration of ethanol is 112g L -1 , and when 1.0g PEG-200 was used to replace the pH solution, the glucose conversion rate increased to 99%, the ethanol yield increased to 88%, and the ethanol concentration increased to 129g L -1 , with the increase of the amount of PEG-200, the glucose conversion rate, ethanol yield a...

Embodiment 10-17

[0043] Weigh 0.4g of yeast into a 100mL Erlenmeyer flask, rehydrate and activate with 4mL ultrapure water at 38°C for 20min, and then use it as distiller's mother. Then add 6.0g glucose, PEG-400 (according to the dosage in Table 2) (1-8g), pre-configured H 2 SO 4 The solution (consumption according to Table 2) was sealed with a plastic wrap, and placed in a shaker for 48 hours at 38° C. with shaking at a speed of 160 r / min. It can be seen from the data in Table 2 that when 4.0g PEG-400 is used to replace the pH solution, the ethanol concentration can reach 150g L -1 , and almost no residual sugar remains in the fermentation system. These results further prove that PEG can replace water as a fermentation medium within a certain proportion, increase the concentration of ethanol at the end of fermentation, reduce the residual glucose content at the end of fermentation, reduce water consumption, reduce production costs, and improve fermentation efficiency.

[0044] Table 2. Eff...

Embodiment 18-25

[0047] Weigh 0.4g of yeast into a 100mL Erlenmeyer flask, rehydrate and activate with 4mL ultrapure water at 38°C for 20min, and then use it as distiller's mother. Then add 6.0g of glucose, PEG-600 (according to the dosage in Table 3) (1-8g), pre-configured H 2 SO 4 The solution (consumption according to Table 3) was sealed with a plastic wrap, and placed in a shaker for 48 hours at 38° C. with shaking at a speed of 160 r / min. It can be seen from the data in Table 3 that when PEG-600 is used to replace the pH solution, the ethanol concentration gradually increases and then decreases with the increase of the amount of PEG-600, and the highest ethanol concentration can reach 150g L -1 . These results fully prove that PEG can replace water as a fermentation medium within a certain proportion, increase the concentration of ethanol at the end of fermentation, reduce the residual glucose content at the end of fermentation, reduce water consumption, reduce production costs, and imp...

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Abstract

The invention relates to a very high gravity (VHG) ethanol fermentation technology by using a surfactant and water as the media, wherein the surfactant enhances the concentration of ethanol generated by yeast fermentation in the water. Glucose is used as a raw material and supplemented with a nonionic surfactant-water mixture as the fermentation medium, and Saccharomyces cerevisiae is inoculated for very high gravity ethanol fermentation without adding any nutritional component. The invention adopts non-ionic using surfactant instead of water to increase the concentration of substrate, and VHG ethanol fermentation on substrate with concentration of 30% is carried out directly, so as to obtain the VHG fermentation end product ethanol. The technology can reduce water consumption and production cost, and increase fermentation efficiency.

Description

technical field [0001] The invention relates to the field of ethanol production by fermentation process, in particular to an ultra-high concentration ethanol fermentation technology using surfactant-water as a medium. Background technique [0002] The rapid development of the world economy has made people more and more dependent on fossil resources, and its high-intensity development and utilization have led to the depletion of its reserves and the soaring price [Progress In Chemistry, 2006, 18 (2-3): 131-141 ]; and the exploitation and use of a large number of fossil resources has brought serious environmental pollution, so it is imperative to develop clean and renewable energy to supplement and replace it. In recent years, countries all over the world have devoted themselves to the development of new energy sources. As a green and renewable new fuel, fuel ethanol has many advantages: ① energy saving and environmental protection; ② increasing the octane number of gasoline; ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12R1/865
CPCC12P7/06Y02E50/10
Inventor 张宗超刘秀梅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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