A sob-r latex enhanced immunoturbidimetric detection kit utilizing surface functional groups

A technology for detecting kits and surface functional groups, which can be used in biological testing, material inspection, etc. It can solve the problems of poor control of sample dilution, narrow linear range, and complicated operation, and achieve shortened reaction time, increased sensitivity, and extended validity period. Effect
CN104459153BActive Publication Date: 2016-03-30CHONGQING ZHONGYUAN BIOLOGICAL TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHONGQING ZHONGYUAN BIOLOGICAL TECH
Publication Date
2016-03-30

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Abstract

The invention relates to the field of in-vitro diagnostic reagents, and particularly relates to a soluble leptin receptor (sOB-R) latex reinforced immunonephelometry assay kit by utilizing a surface functional group. The assay kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from a buffer solution 1, a stabilizing agent 1, a preservative 1, EDTA, a gelling agent and a protective agent 1; the reagent R2 is prepared from polystyrene latex microspheres crosslinked with an sOB-R antibody, a buffer solution 2, a stabilizing agent 2, a preservative 2 and a protective agent 2; the polystyrene latex microspheres and the sOB-R antibody are connected by means of covalent cross-linking; the surface functional group of the polystyrene latex microspheres in the reagent R2 is amino, carboxyl, hydrazide, aldehyde or epoxy group. According to the assay kit, a latex reinforced immunonephelometry method is adopted, when the sOB-R in blood reacts with the sOB-R antibody, polystyrene latex is driven to aggregate and generate certain turbidity, so that the detection can be convenient. The sOB-R latex reinforced immunonephelometry assay kit is easily applied in clinical application.
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Description

technical field

[0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a sOB-R latex-enhanced immune turbidity detection kit utilizing surface functional groups. Background technique

[0002] Soluble leptin receptor (sOB-R) is the most important leptin-binding protein in blood circulation. In the natural state, there are two sizes of leptin-binding protein in human blood, with molecular weights of 140kD and 110kD. After deglycosylation by glycosidase, the molecular weights are 90kD and 60kD. In the human body, soluble leptin receptors mainly come from transmembrane leptin Shedding of the receptor, which has a crucial role in regulating leptin signaling function. sOB-R only exists in the peripheral blood circulation of normal people, and sOB-R has not been found in the cerebrospinal fluid. Under physiological conditions, sOB-R has obvious circadian rhythm, and the level of sOB-R increases when hungry. In healthy people, sOB-R is positiv...

Claims

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