Lactobacillus plantarum boosting immunological competence

A Lactobacillus plantarum, immune-enhancing technology, applied in the biological field, can solve problems such as large differences in probiotic activity

Active Publication Date: 2015-04-01
海泰合加(天津)信息科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strain-specific probiotic activity of lactic acid

Method used

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  • Lactobacillus plantarum boosting immunological competence
  • Lactobacillus plantarum boosting immunological competence
  • Lactobacillus plantarum boosting immunological competence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation and cultivation of bacterial strain

[0029] Collect traditional Chinese kimchi, grind it, dilute it with sterile normal saline gradient to an appropriate concentration, spread it on the MRS plate medium by the plate coating method, and culture it statically at 37°C for 36-72 hours to obtain Lactobacillus Individual colonies of suspected strains. The colonies were spread on the MRS plate medium, and anaerobically cultured at 37°C for 36-72 hours, and a single colony was picked for further purification. The strain identification was carried out according to the following Example 2, and the isolated strain BC299 was obtained.

[0030] Inoculate the isolated strains on the MRS plate medium by streaking method, culture anaerobically at 37°C for 36-72 hours, pick a single colony into 0.5-2mL fresh sterile MRS liquid medium, and incubate at 37±2°C Anaerobic culture for 24-48 hours, after the strain grows well, inoculate 5-8mL fresh sterile MRS liquid ...

Embodiment 2

[0031] Embodiment 2: bacterial strain identification

[0032] 2.1. Preliminary Analysis

[0033] Carry out preliminary analysis according to the standard method of strain identification, the result shows that isolated bacterial strain BC299 of the present invention is Gram-positive, catalase-negative bacillus (attached figure 1 ), no motility, no spore production, and can grow under both aerobic and anaerobic conditions.

[0034] 2.2 PCR analysis of 16S rDNA

[0035] Using the genomic DNA of strain BC299 as a template, add bacterial universal primers (P1 sequence: 5'-AGAGTTTGATCCTGGCTCAG-3'; P2 sequence: 5'-AAGGAGGTGATCCAGCC-3'), DNA polymerase, buffer, dNTPs to the PCR tube After waiting for the reagents, perform PCR amplification.

[0036] The amount of each reagent added and the amplification procedure are as follows:

[0037] Addition amount of each reagent: DNA genome (100ng / μL) 1μL as PCR reaction template, 0.5mmol / L primers P1 and P2 each 1μL, 10×PCR buffer (contain...

Embodiment 3

[0046] Embodiment 3: the acid resistance and bile salt resistance test of Lactobacillus plantarum BC299

[0047] In order to test whether Lactobacillus plantarum BC299 can successfully pass through the acidic and high bile salt environment of the gastrointestinal tract, so as to successfully exert its function of improving immunity in the intestinal tract, the experimental procedure is as follows:

[0048] After continuous activation of Lactobacillus plantarum BC299 in MRS liquid medium until rejuvenated, inoculate 4% (v / v) inoculum in MRS liquid medium with a pH value of 2.0, and perform static anaerobic culture at 37°C. Sampling was carried out at 0, 30, 60, 90, and 120 minutes respectively, and the gradient was diluted to 10 -5 ~10 -7 Afterwards, colony counting (mean ± SD) was carried out by the plate coating method to determine the tolerance of the strains to gastric acid.

[0049] In addition, the activated Lactobacillus plantarum BC299 was inoculated in the MRS liquid...

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Abstract

The invention relates to lactobacillus plantarum boosting immunological competence and a composition comprising the bacterial strain. The bacterial strain is lactobacillus plantarum with an accession number of CGMCC6326. The bacterial strain can boost body immunity. The composition is in a form of a food or medicine composition. The invention also discloses a method of screening and measuring the lactobacillus strain, and a method of boosting body immunity by increasing the visceral index regulating bodies, and nonspecific immunity and specific immunity reactions. The objective of the lactobacillus plantarum, the composition and the methods is that: daily intake of probiotic lactic acid bacteria is adopted to replace medicines to boost immunity of human bodies, and lactic acid bacteria which are free of toxic and side effects to human bodies and beneficial to health are adopted as a new choice for treating patients with hypoimmunity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a plant lactobacillus with immune regulation activity. Background technique [0002] Probiotics refer to a class of active microorganisms that have beneficial effects on the host. By colonizing the host's intestinal tract and reproductive system, they can produce definite health effects on the host, thereby improving the micro-ecological balance in the host and exerting beneficial effects. general term. As the most representative genus of probiotics, lactic acid bacteria can help digestion, promote the absorption of nutrients, relieve lactose intolerance, prevent and treat diarrhea, regulate intestinal flora and relieve constipation, regulate the body's immune function, reduce blood Total cholesterol, improvement of allergic diseases caused by food, anti-aging and anti-tumor and other physiological activities. [0003] With the growth of age, the increasing pressure of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P37/04A23L1/29C12R1/25A23L33/00
CPCA61K35/742A23L33/135C12N1/205C12R2001/25
Inventor 王艳萍
Owner 海泰合加(天津)信息科技有限公司
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