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A selenium-enriched lactic acid bacteria preparation for improving the meat color of broiler chicken and its application

A technology of lactic acid bacteria and preparations, applied in the application of selenium-enriched lactic acid bacteria preparations and the preparation of selenium-enriched lactic acid bacteria preparations, can solve problems such as the combined application of selenium and lactic acid bacteria, which has not been seen, and achieves improved antioxidant capacity, improved nutritional value, and improved content. Effect of Selenium Amount

Active Publication Date: 2017-12-22
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, there have been research reports on the use of selenium or lactic acid bacteria alone to improve the quality of meat color; but there has not been a patent or report on the joint application of selenium and lactic acid bacteria to improve the meat color of broiler chickens, and there is no biotransformation of lactic acid bacteria. Patents or reports on the conversion of inorganic selenium into organic selenium and the preparation of selenium-enriched lactic acid bacteria, which are then applied to improve the meat color of broiler chicken muscles

Method used

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  • A selenium-enriched lactic acid bacteria preparation for improving the meat color of broiler chicken and its application
  • A selenium-enriched lactic acid bacteria preparation for improving the meat color of broiler chicken and its application
  • A selenium-enriched lactic acid bacteria preparation for improving the meat color of broiler chicken and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation method of selenium-enriched lactic acid bacteria preparation for improving broiler muscle quality:

[0029] (1) Strain: Lactobacillus rhamnosus ( L.rhamnosus ): purchased from the Institute of Microbiology, Chinese Academy of Sciences and kept by our laboratory;

[0030] (2) Strain activation medium: peptone 10 g, yeast extract 5 g, glucose 20 g, beef extract 10 g, sodium acetate 5 g, diammonium citrate 2 g, Tween 80 1 mL, MgSO 4 ·7H 2 O 0.58 g, MnSO 4 ·4H 2 O 0.05 g, K 2 HPO 4 2 g, agar powder 20 g, water 1000 mL, adjust pH=6.2-6.4; autoclave at 121 ℃ for 20 min, spare;

[0031] (3) Seed culture medium: peptone 10 g, yeast extract 5 g, glucose 20 g, beef extract 10 g, sodium acetate 5 g, diammonium citrate 2 g, Tween 80 1 mL, MgSO 4 ·7H 2 O 0.58 g, MnSO 4 ·4H 2 O 0.05 g, K 2 HPO 4 2 g, 1000 mL of water, adjust pH=6.2-6.4; autoclave at 121 ℃ for 20 min, standby;

[0032] (4) Preparation production medium: glucose 150 g / L, yeast powder 5 / L, peptone 10 g / L, beef extr...

Embodiment 2

[0038] Preparation method of selenium-enriched lactic acid bacteria preparation for improving broiler muscle quality:

[0039] (1) Strain: Lactobacillus rhamnosus ( L.rhamnosus ): purchased from the Institute of Microbiology, Chinese Academy of Sciences and kept by our laboratory;

[0040] (2) Strain activation medium: peptone 10 g, yeast extract 5 g, glucose 20 g, beef extract 10 g, sodium acetate 5 g, diammonium citrate 2 g, Tween 80 1 mL, MgSO 4 ·7H 2 O 0.58 g, MnSO 4 ·4H 2 O 0.05 g, K 2 HPO 4 2 g, agar powder 20 g, water 1000 mL, adjust pH=6.2-6.4; autoclave at 121°C for 30 min, for use;

[0041] (3) Seed culture medium: peptone 10 g, yeast extract 5 g, glucose 20 g, beef extract 10 g, sodium acetate 5 g, diammonium citrate 2 g, Tween 80 1 mL, MgSO 4 ·7H 2 O 0.58 g, MnSO 4 ·4H 2 O 0.05 g, K 2 HPO 4 2 g, 1000 mL of water, adjust pH=6.2-6.4; autoclave at 121 ℃ for 30 min, standby;

[0042] (4) Preparation medium: glucose 50 g / L, yeast powder 20 g / L, peptone 30 g / L, beef extract 30...

Embodiment 4

[0075] Applications

[0076] A broiler farm in Qingdao City, Shandong Province

[0077] 1. Preparation of selenium-enriched lactic acid bacteria preparation:

[0078] (1) Strain: Lactobacillus rhamnosus ( L.rhamnosus ): purchased from the Institute of Microbiology, Chinese Academy of Sciences, and kept by our laboratory.

[0079] (2) Strain activation medium: peptone 10 g, yeast extract 5 g, glucose 20 g, beef extract 10 g, sodium acetate 5 g, diammonium citrate 2 g, Tween 80 1 mL, MgSO 4 ·7H 2 O 0.58 g, MnSO 4 ·4H 2 O 0.05 g, K 2 HPO 4 2 g, agar powder 20 g, water 1000 mL, adjust pH=6.2-6.4; autoclave at 121 ℃ for 30 min, and use it for later use.

[0080] (3) Seed culture medium: peptone 10 g, yeast extract 5 g, glucose 20 g, beef extract 10 g, sodium acetate 5 g, diammonium citrate 2 g, Tween 80 1 mL, MgSO 4 ·7H 2 O 0.58 g, MnSO 4 ·4H 2 O 0.05 g, K 2 HPO 4 2 g, 1000 mL of water, adjust pH=6.2-6.4; autoclave at 121 ℃ for 30 min, set aside.

[0081] (4) Preparation medium: glucose 10...

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Abstract

The invention discloses a selenium-enriched lactic acid bacteria preparation for improving the broiler muscle color and an application of the preparation. A specific culture medium with an organic selenium conversion accelerator added is inoculated with lactobacillus rhamnosus used as a strain; an inorganic selenium source is added after culture for 15-21 h, and culture is continuously performed for 27-36 h; after culture, the selenium-enriched lactic acid bacteria preparation is obtained through drying. The number of lactic acid bacteria in the preparation is larger than 109 cfu / g, the selenium content is in a range of 5.2-22.8 mg / kg, and the organic selenium content is higher than 50%. The preparation is added to broiler feed in the additive amount of 2-5%, so that the index brightness and the redness value of the broiler muscle color as well as the selenium content and the oxidation resistance of muscle tissue can be significantly improved, and the preparation can effectively improve the broiler muscle color. The preparation has the broad application prospect in the aspect of improving the animal muscle quality.

Description

Technical field [0001] The invention relates to the fields of food biotechnology and feed additive production, in particular to a preparation method of a selenium-enriched lactic acid bacteria preparation for improving the flesh color of broiler chickens, and the application of the selenium-enriched lactic acid bacteria preparation. Background technique [0002] With the improvement of people's living standards and awareness of health care, people's pursuit of food has risen from the demand for quantity to the demand for quality, especially for animal food. As an important part of the diet, livestock and poultry meat must not only meet the needs of the people in quantity, but also meet the higher-level needs of the people in terms of meat "quality" such as taste, meat color, drip loss, and nutritional components. . Improving the quality of livestock and poultry meat through various ways is one of the hotspots in the field of food science and animal science. [0003] Meat quality ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75C12R1/225
CPCC12N1/20
Inventor 秦顺义黄宝霞马吉飞金天明张建斌刘田生罗安智陈甫王宏祥
Owner TIANJIN AGRICULTURE COLLEGE