Hyperthermophilic glycosidase mutant and application thereof in preparation of ginsenoside CK

Abstract

The invention relates to a hyperthermophilic glycosidase mutant and an application thereof in preparation of ginsenoside CK, and belongs to the technical field of biotechnology engineering. An amino acid sequence of the hyperthermophilic glycosidase mutant is as shown in SEQ ID NO:2; and the hyperthermophilic glycosidase mutant is applied to preparation of rare ginsenoside CK. The hyperthermophilic glycosidase mutant has high heat resistance and stability; relatively high catalytic activity can still be kept after heat preservation at 70-80 DEG C for over 300 hours, and thus hyperthermophilic glycosidase mutant has the advantages of low storage and transportation cost, and low requirement standards on a cooling system of a reactor when applied to production; the kinetic reaction is accelerated; meanwhile, the hyperthermophilic glycosidase mutant has beta-glucosaccharase activity; glucosides of main protopanaxadiol type saponins C3 site and C20 site can be hydrolyzed on the basis that the saponin structure is not destroyed; and the hyperthermophilic glycosidase mutant is efficient, specific, few in byproducts, high in yield and the like, so that the Fpglula can be applied to industrial production of a rare ginsenoside Compound K.

Description

technical field

[0001] The invention belongs to the technical field of biotechnology engineering, and in particular relates to a hyperthermophilic glycosidase mutant gene derived from bacteria of the genus Fervidobacterium, a recombinant vector, a genetically engineered bacterium for producing a mutant, and the hyperthermophilic glycosidase mutation Application of body in preparation of rare ginsenoside CK. Background technique

[0002] Ginseng (Panax ginseng) is a traditional Chinese herbal medicine with a long history. Chinese traditional medicine believes that ginseng can "replenish the five internal organs, soothe the spirit, soothe the soul, stop palpitations, eliminate evil spirits, improve eyesight, improve intelligence, make light of body and prolong life after long-term use". Ginsenoside is the main active substance in ginseng, and more than 100 kinds of natural ginsenoside products have been found in ginseng, and more than 40 kinds have been isolated. The content...

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