Ultrafine-grinding assisted enzymatic-hydrolysis based preparation method of wheat bran oligosaccharides
A technology of ultrafine grinding and wheat bran, which is applied in the field of food processing, can solve the problems of environmental pollution, high yield of oligosaccharides, and many side reactions, and achieve the goals of reducing environmental pollution, improving enzymatic hydrolysis efficiency, and improving utilization rate Effect
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[0033] Example 1
[0034] A method for preparing wheat bran oligosaccharides by superfine pulverization assisted enzymatic method, the specific steps are as follows:
[0035] (1) Superfine pulverization: ordinary pulverized wheat bran is superfinely pulverized into wheat bran powder with a particle size of less than 100 microns;
[0036] (2) Preparation of oligosaccharide by compound enzymatic hydrolysis: mix the ultra-finely pulverized wheat bran powder with water at a mass ratio of 1:8 to obtain wheat bran slurry; use high-temperature resistant α-amylase and glucoamylase to degrade the wheat bran slurry Starch (high temperature resistant α-amylase enzymatic hydrolysis conditions: pH 5.0, temperature 90°C, enzyme addition 100U / g, time 10min; glucoamylase enzymatic hydrolysis conditions: pH 4, temperature 60°C, enzyme addition 80U / g, time 20min); Use alkaline protease to degrade protein (Enzymatic hydrolysis conditions: pH 7, temperature 50℃, add enzyme amount 0.015AU / g, time 10min)...
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[0038] Example 2
[0039] A method for preparing wheat bran oligosaccharides by superfine pulverization assisted enzymatic method, the specific steps are as follows:
[0040] (1) Superfine pulverization: ordinary pulverized wheat bran is superfinely pulverized into wheat bran powder with a particle size of less than 100 microns;
[0041] (2) Preparation of oligosaccharides by compound enzymatic hydrolysis: mix ultra-finely pulverized wheat bran powder and water at a mass ratio of 1:20 to obtain wheat bran slurry; use high-temperature resistant α-amylase and glucoamylase to degrade the wheat bran slurry Starch (high temperature resistant α-amylase enzymatic hydrolysis conditions: pH 7.0, temperature 100°C, enzyme amount 110U / g, time 30min; saccharification enzyme enzymatic hydrolysis conditions: pH 6, temperature 70°C, enzyme amount 90U / g, time 30min); Use alkaline protease to degrade protein (Enzymatic hydrolysis conditions: pH 8, temperature 70℃, add enzyme amount 0.02AU / g, time 30...
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[0043] Example 3
[0044] A method for preparing wheat bran oligosaccharides by superfine pulverization assisted enzymatic method, the specific steps are as follows:
[0045] (1) Superfine pulverization: ordinary pulverized wheat bran is superfinely pulverized into wheat bran powder with a particle size of less than 100 microns;
[0046] (2) Preparation of oligosaccharides by compound enzymatic hydrolysis: mix ultra-finely pulverized wheat bran powder and water at a mass ratio of 1:12 to obtain wheat bran slurry; use high-temperature resistant α-amylase and glucoamylase to degrade the wheat bran slurry Starch (High temperature resistant α-amylase enzymatic hydrolysis conditions: pH 6.0, temperature 95°C, enzyme amount 110U / g, time 10min; glucoamylase enzymatic hydrolysis conditions: pH 5, temperature 60°C, enzyme amount 90U / g, time 20min); Use alkaline protease to degrade protein (Enzymatic hydrolysis conditions: pH 7, temperature 60℃, add enzyme amount 0.02AU / g, time 10min); Use ce...
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