Method for coagulating natural latex by using compound microorganisms

A technology of combining microorganisms and natural latex, applied in the field of coagulation of natural latex, can solve the problems of not considering the interaction between anaerobic microorganisms and aerobic microorganisms, incomplete coagulation, long coagulation time of latex, etc. Pollution, low cost effect

Inactive Publication Date: 2015-04-08
GUANGDONG OCEAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the methods reported above took into account the interaction between anaerobic and aerobic microorganisms during the latex coagulation process, resulting in longer coagulation time and / or incomplete coagulation of the latex. At present, latex microbial coagulation has not been fully applied in rubber production. craft

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Take 10g peptone, 3g beef extract, 2g sodium chloride, dissolve it in 985ml distilled water, adjust the pH to 7.4 with sodium hydroxide solution, divide into four conical flasks, autoclave at 121℃ for 15min, prepare Liquid culture medium, keep cold for later use. Lactobacillus was inoculated in a liquid medium and cultured on a shaker, and fermented at 36°C for 48h to prepare a first-level culture solution of Lactobacillus. Take 50g of molasses to make 5kg of culture solution, inoculate Lactobacillus primary culture solution in the culture solution, the inoculation amount is 250ml, cover and seal culture at 25~35℃ for 3 days to prepare the use solution of Lactobacillus;

[0024] (2) Take 10g glucose, 10g peptone, 2g sodium chloride, 0.3g beef extract, dissolve it in 977.7ml distilled water, divide it into four conical flasks, autoclave at 121°C for 15min, prepare a liquid medium, put Cold standby. Bacillus natto was inoculated into a liquid medium for shaking culture...

Embodiment 2

[0027] (1) Take 20g peptone, 5g beef extract, 5g sodium chloride, dissolve it in 970ml distilled water, adjust the pH to 7.4 with sodium hydroxide solution, divide it into four conical flasks, autoclave at 121℃ for 15min, prepare Liquid culture medium, keep cold for later use. Lactobacillus was inoculated in a liquid medium and cultured on a shaker, and fermented at 36°C for 48h to prepare a first-level culture solution of Lactobacillus. Take 250g of molasses to prepare 5kg of culture solution, inoculate the primary culture solution of Lactobacillus into the culture solution, the inoculation amount is 250ml, cover and seal culture at 25~35℃ for 3 days to prepare the use solution of Lactobacillus;

[0028] (2) Take 20g glucose, 20g peptone, 5g sodium chloride, 0.5g beef extract, dissolve it in 955ml distilled water, divide into four conical flasks, autoclave at 121℃ for 15min, prepare liquid culture medium, let it cool spare. Bacillus natto was inoculated into a liquid medium fo...

Embodiment 3

[0031] (1) Take 10g peptone, 3g beef extract, 2g sodium chloride, dissolve it in 985ml distilled water, adjust the pH to 7.4 with sodium hydroxide solution, divide into four conical flasks, autoclave at 121℃ for 15min, prepare Liquid culture medium, keep cold for later use. Lactobacillus was inoculated in a liquid medium and cultured on a shaker, and fermented at 36°C for 48h to prepare a first-level culture solution of Lactobacillus. Take 250g of molasses to prepare 5kg of culture solution, inoculate the primary culture solution of Lactobacillus into the culture solution, the inoculation amount is 150ml, cover and seal culture at 25~35℃ for 10 days to prepare the use solution of Lactobacillus;

[0032] (2) Take 10g glucose, 10g peptone, 2g sodium chloride, 0.3g beef extract, dissolve it in 977.7ml distilled water, divide it into four conical flasks, autoclave at 121°C for 15min, prepare a liquid medium, put Cold standby. Bacillus natto was inoculated into a liquid medium for s...

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PUM

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Abstract

The invention discloses a method for coagulating natural latex by using compound microorganisms. The method comprises the following steps: inoculating a liquid culture medium against an anaerobic bacterium and an aerobic bacterium, and culturing the anaerobic bacterium and the aerobic bacterium in a shaker to obtain a primary culture liquid, wherein the anaerobic bacterium is lactobacillus bulgaricus, and the aerobic bacterium is bacillus natto; inoculating a molasses culture medium against the primary culture liquid, culturing the primary culture liquid to obtain a microbial preparation, and coagulating the natural latex by using the microbial preparation. The selected compound microorganisms can act synergistically and complementarily and can be used for coagulating the natural latex under the condition that the content of ammonia is less than 0.1%. A small amount of acids are adopted by the method, and little pollution is caused to environment. The microorganisms are wide in source, are easy to culture, are low in cost and are suitable for popularization.

Description

Technical field [0001] The invention relates to a method for coagulating natural rubber latex, in particular to a method for coagulating natural rubber latex by combining microorganisms, and belongs to the technical field of natural rubber latex coagulation. Background technique [0002] The natural rubber latex flowing out of the rubber tree needs to be coagulated into a clot before it can enter the process of thinning, crepe dehydration, hammer milling and granulation and drying, and finally make a standard rubber product. According to the different types of coagulants, the coagulation process of latex is mainly divided into acid coagulation, natural coagulation, inorganic salt coagulation and microorganism coagulation. The latex flowing from the rubber tree will coagulate naturally after being left for a period of time, but the coagulation speed is slow. By adding the substances required for the reproduction of microorganisms, the activity of the contaminated microorganisms a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08C1/15C12N1/20C12R1/07C12R1/225
Inventor 钟杰平林宏图彭政许逵杨磊李思东陈亚胜廖禄生张福全邓东华汪月琼杨昌金
Owner GUANGDONG OCEAN UNIVERSITY
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